Absorbance Liquid Test 1 - Biotek Synergy HT Operator's Manual

Absorbance Liquid Test 1

Materials
Manufacturer part numbers are subject to change.
•
New 96-well, clear, flat-bottom microplate (Corning Costar #3590
recommended)
•
Stock Solution A or B, which may be formulated by diluting a dye solution
available from BioTek (A) or from the ingredients listed below (B).
Solution A
•
BioTek QC Check Solution No. 1 (PN 7120779, 25 mL; PN 7120782, 125 mL)
•
Deionized water
•
5-mL Class A volumetric pipette
•
100-mL volumetric flask
1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL
volumetric flask.
2. Add 95 mL of DI water; cap and shake well. The solution should measure
approximately 2.000 OD when using 200 µL in a flat-bottom microwell.
Solution B
•
Deionized water
•
FD&C Yellow No. 5 dye powder (typically 90% pure)
•
Tween 20 (polyoxyethylene (20) sorbitan monolaurate)
agent (PN 7773002) (a 10% Tween solution)
•
Precision balance with capacity of 100 g minimum and readability of 0.001 g
•
Weigh boat
•
1-liter volumetric flask
1. Weigh out 0.092 g of FD&C Yellow No. 5 dye powder into a weigh boat.
2. Rinse the contents into a 1-liter volumetric flask.
3. Add 0.5 mL of Tween 20, or 5 mL of BioTek's wetting agent.
4. Fill to 1 liter with DI water; cap and shake well. The solution should
measure approximately 2.000 OD when using 200 µL in a flat-bottom
microwell.
Synergy HT Operator's Manual
Absorbance Liquid Tests |
or BioTek wetting
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