Biotek Synergy HT Operator's Manual page 61

Removing the Filter Wheels
The filter wheels can be removed if their contents need to be changed.
It is important to note that:
•
The Excitation and Emission filter wheels are not interchangeable
and are labeled as follows: EX = Excitation, EM = Emission.
(TR = Time-Resolved Cartridge; see page 39.)
•
Filter direction within a filter wheel is important, and the direction differs
depending on the filter wheel. There is a diagram on the inside of the front
panel door indicating this.
•
Each filter is marked with an arrow indicating the proper direction of light.
Refer to the figures on the previous page for proper filter orientation.
To remove a filter wheel:
1. Important!
2. Using your thumbs, push down on the bottom corners of the hinged door
on the front of the instrument.
3. Observe the two thumbscrews within the compartment. The left
thumbscrew holds the Excitation filter wheel in place; the right secures the
Emission filter wheel.
4. Remove the thumbscrew and slide the filter wheel's supporting metal
bracket straight out of the compartment.
will "spring" out when removed. (This is because a shutter behind the
wheel closes quickly to protect the PMT.)
Synergy HT Operator's Manual
Important! The Synergy HT is shipped with a set of Excitation
and Emission filters installed, and the Synergy's onboard
software is pre-configured with the filter values and their
locations.
If you change the contents of a filter wheel, you must update
Gen5's filter table and then download the information to the
reader. The Synergy does not automatically detect which filters
are installed.
See page 44 for information on updating Gen5's filter table.
Turn off the instrument.
Key Components |
Note: The Emission filter wheel
37