Biotek Synergy HT Operator's Manual page 195

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During the validation of the syringe calibration data the μL/step failed
2C03
This error indicates that when the software calculated the μl/step, the results were either
< 0.0100 or > 9.9999.
Note:
Probable Causes:
• Values entered are corrupted or incorrect.
2C05 During the validation of the syringe calibration data, the calibration data failed
This error can indicate one of the following error scenarios:
• The minimum calibration value is not equal to 5.
• The minimum measured value is not between 2 and 6.
• The second measured value is not within ± 20% of the calibrated value.
• The third through fifth measured values are not within ± 10% of the calibrated value.
• The calibration values are not in ascending order.
• The measured calibration values are not in ascending order.
Note:
Probable Causes:
• Values entered are corrupted or incorrect.
• Incorrect calibration of the syringe.
Default calibration data set to "do not use"
2C07
This error indicates that the default calibration data flag is set. Syringe needs to be calibrated.
Note:
Probable Causes:
• Factory default data is set; the syringe needs to be calibrated before use, or calibration
values need to be entered from the syringe drive.
• Values entered are corrupted or incorrect.
2D00 During a dispense read the reader missed the start of the well read.
This error indicates that during a dispense read, the reader was not able to start the read for a
particular well.
Probable Causes:
• The processor was busy running other tasks.
• A previous task took longer than estimated.
2D02 Filter bandpass overlap in intermediate switching position for multi-filter set reads
This error indicates that the software cannot move either the EX or EM filter wheel without
causing the filters to overlap and saturate the PMT.
Note:
Equation used for overlapping:
If EM wavelength is > EX
if (EX wavelength + ½ bandpass) >= (EM wavelength – ½ bandpass) then fail
Else if EX wavelength is >EM
if (EM wavelength + ½ bandpass) >= (EX wavelength – ½ bandpass) then fail
Probable Causes:
• The filters placed in the filter wheels are not in the optimum order to prevent this error.
• The filters defined in the filter table do not match the filter wheels.
• The user did not send the filter table information to the reader after making changes to the
table in the controlling software.
Synergy HT Operator's Manual
Description and Probable Causes:
This error can apply to either Syringe 1 or Syringe 2.
This error can apply to either Syringe 1 or Syringe 2.
This error can apply to either Syringe 1 or Syringe 2.
Only 2 filter sets can be sent when developing software.
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Error Codes |