Biotek Synergy H1 Operator's Manual
  1. Manuals
  2. Brands
  3. Biotek Manuals
  4. Laboratory Equipment
  5. Synergy H1
  6. Operator's manual

Biotek Synergy H1 Operator's Manual

Hybrid multi-mode microplate reader
Hide thumbs Also See for Synergy H1:
Table of Contents

Advertisement

Quick Links

Hybrid Multi-Mode Microplate Reader
 
Synergy H1
TM
Operator's Manual
 

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Synergy H1 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Biotek Synergy H1

Summary of Contents for Biotek Synergy H1

  • Page 1 Hybrid Multi-Mode Microplate Reader   Synergy H1 Operator’s Manual  ...
  • Page 2  ...
  • Page 3 Synergy H1 Multi-Mode Reader Operator's Manual ® BioTek Instruments, Inc. Part Number 8041000 Revision K May 2016 © 2016...

  • Page 4: Notices

    BioTek Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

  • Page 5: Table Of Contents

    Directive 98/79/EC: In Vitro Diagnostics (if labeled for this use) Electromagnetic Interference and Susceptibility USA FCC CLASS A Canadian Department of Communications Class A User Safety Safety Symbols xxii Introduction Product Description Package Contents & Accessories Optional Accessories Materials for Conducting Liquid Tests Synergy H1 Operator's Manual...
  • Page 6 Technical Assistance Center (TAC) Applications Support Installation Product Registration Important Information 1: Unpack and Inspect the Synergy H1 2: Select an Appropriate Location 3: Remove the Shipping Hardware 4: Install the Power Supply 5: Install the Gas Controller 6: Unpack and Inspect the Dispense Module...
  • Page 7 Adding a Filter Cube to the Gen5 Optics Library Handling Filters and Mirrors Change a Filter or Mirror Clean the Filters and Mirrors Filters and Mirrors Available from BioTek Preventive Maintenance Overview Daily Cleaning for the Dispense Module Recommended Maintenance Schedule...
  • Page 8 Dispense Module, Syringe Replacement Syringe Maintenance Position Replace the Syringe Instrument Qualification Process Instrument System Test Plate Shaker Test Absorbance Testing BioTek Absorbance Test Plates Test Methods Sample Test Report Troubleshooting Peak Absorbance Test Alignment Test Accuracy Test Repeatability Test...
  • Page 9 Recommended Qualification Schedule System Test Setup Test Procedure Absorbance Plate Tests Requirements Setup Test Procedure Absorbance Liquid Tests Absorbance Liquid Test 1 Materials Solution A Solution B Test Procedure Absorbance Liquid Test 2 Materials Test Procedure Synergy H1 Operator's Manual...
  • Page 10 Alternate/Supplemental Tests Using Methylumbelliferone (MUB) Materials Test Solutions Test Procedure Pipette Map Injection System Tests Materials Alternate Test Solutions Test Procedure for Models with Absorbance Capability Test Procedure for Models without Absorbance Capability Specifications General Specifications Microplates BioTek Instruments, Inc.
  • Page 11 Absorbance Specifications Optics Performance Accuracy Linearity Repeatability Fluorescence Specifications Monochromator-Based Fluorescence Performance Filter-Based Fluorescence Fluorescence Intensity Time-Resolved Fluorescence Fluorescence Polarization Luminescence Specifications Dispense/Read Specifications Error Codes Overview Error Codes Instrument Dimensions for Robotic Interface Sample Reports Synergy H1 Operator's Manual...

  • Page 12: Contact Information

    Winooski, Vermont 05404-0998 USA Global Service and Support BioTek product service and repair is available worldwide at one of BioTek's International Service Centers and in the field at your location. To arrange for service or repair, contact the office nearest you; visit www.biotek.com for up-to-date contact information. For customer service, sales, and technical assistance, refer to the information below.

  • Page 13: Revision History

    Appendix A: Specifications: Corrected Incubation specification. 11/2011 Chapter 2: Installation: Updated the Carrier Shipping Bracket photo in Figure 1. Updated the BioTek part number for the shipping hardware. Updated the Dispense Module installation instructions. Chapter 7: Instrument Qualification: For the Absorbance Plate Test, removed the restriction on the use of the peak closest to 243 nm for the Erbium glass (any peak may be used).
  • Page 14 Chapter 3, Getting Started: Added a note stating that the workarounds for kinetic assays with continuous shake apply to Synergy H1 basecode software versions lower than 2.00 (the issue was addressed in v2.00). Chapter 7, Instrument Qualification: Corrected the PN for the pre-configured qualification TRF filter cube (8040555).
  • Page 15 Added information for purchasing and using the BioTek 340 nm Absorbance Test Plate (BTI #7260551). 5/2016 Added information on using the BioTek Fluorescence Test Plate to qualify the Synergy H1 fluorescence system. Updated the Fluorescence Intensity and TRF liquid tests to utilize a single concentration well and median buffer well when determining the detection limit.

  • Page 16: Document Conventions

    "Applies only to models equipped with injectors." Intended Use Statement The Synergy H1 is a hybrid multi-mode microplate reader. The performance characteristics of the data reduction software have not been established with any laboratory diagnostic assay. The user must evaluate this instrument and PC-based software in conjunction with their specific assay(s).

  • Page 17: Quality Control

    Customer Resource Center at www.biotek.com or call (888) 451-5171 or (802) 655-4740. Repackaging and Shipping If you need to ship the instrument to BioTek for service or repair, contact BioTek for a Service Call Notice (SCN) number, and be sure to use the original packing materials.

  • Page 18: Warnings

    Warning! Accessories. Only accessories that meet the manufacturer’s specifications shall be used with the instrument. Warning! The instrument with all available modules weighs up to 55 pounds (24.95 kg). Use two people when lifting and carrying the instrument. BioTek Instruments, Inc.

  • Page 19: Precautions

    If a spill occurs while a program is running, abort the program and turn the instrument off. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid; contact BioTek TAC. Warning! Unspecified Use. Failure to operate the equipment according to the guidelines and safeguards specified in this manual could result in a hazardous condition.
  • Page 20 Caution: Electromagnetic Compatibility. Do not use this device in close proximity to sources of strong electromagnetic radiation (e.g., unshielded intentional RF sources), because these may interfere with the proper operation. BioTek Instruments, Inc.

  • Page 21: Ce Mark

    EN 61010-1, “Safety requirement for electrical equipment for measurement, control and laboratory use. Part 1, General requirements.” EN 61010-2-081, “Particular requirements for automatic and semi-automatic laboratory equipment for analysis and other purposes.” EN 61010-2-010, “Particular requirements for laboratory equipment for the heating of materials.” Synergy H1 Operator's Manual...

  • Page 22: Directive 2012/19/Eu: Waste Electrical And Electronic Equipment

    Communications. Le present appareil numerique n'émet pas de bruits radioélectriques depassant les limites applicables aux appareils numérique de la Class A prescrites dans le Réglement sur le brouillage radioélectrique édicté par le ministère des Communications du Canada. BioTek Instruments, Inc.

  • Page 23: User Safety

    Part 1: General requirements.” Canadian Standards Association CAN/CSA C22.2 No. 61010-1, “Safety requirements for electrical equipment for measurement, control and laboratory use; Part 1: General requirements.” EN 61010 Standards, see CE Mark starting on page xix. Synergy H1 Operator's Manual...

  • Page 24: Safety Symbols

    Off (Supply) Caution (refer to accompanying documents) Arrêt (alimentation) Attention (voir documents Aus (Trennung vom Netz) d’accompagnement) Desconectado Achtung siehe Begleitpapiere Aperto (sconnessione dalla Atención (vease los documentos rete di alimentazione) incluidos) Attenzione, consultare la doc annessa BioTek Instruments, Inc.
  • Page 25 Rayonnement laser: Ne pas regarder Gebrauchsanweisung dans le faisceau beachten Laserstrahlung: Nicht in den strahl Consultar las instrucciones blicken de uso Radiación de láser: No mire Consultare le istruzioni per fijamente al rayo Radiazione di laser: Non stare nel fascio Synergy H1 Operator's Manual...
  • Page 26 | Preface BioTek Instruments, Inc.

  • Page 27: Introduction

    Chapter 1 Introduction This chapter introduces the Synergy H1 Multi-Mode Reader, describes its key features, lists its package contents, and provides contact information for technical assistance. Product Description Package Contents & Accessories Optional Accessories Materials for Conducting Liquid Tests Product Support & Service...

  • Page 28: Product Description

    BioStack Microplate Stacker. Gen5 supports OLE automation to facilitate integration into an automated system. The Synergy H1 can perform reads using a filter cube or a monochromator*. The filter- based system can perform fluorescence and luminescence reads. Filter fluorescence uses a xenon flash light source, along with interference filters and dichroic mirrors for wavelength specificity and a photomultiplier tube (PMT) detector.

  • Page 29: Package Contents & Accessories

    Package Contents & Accessories | 3 Package Contents & Accessories Package contents and part numbers are subject to change. Please contact BioTek Customer Care with any questions. Item BTI Part # Synergy H1 Operator's Manual (on USB flash drive) 8041000 Power supply...

  • Page 30: Optional Accessories

    Additional filters and filter cubes; contact BioTek Customer Care for availability and part numbers The Synergy H1 is compatible with the BioStack Microplate Stacker. The BioStack rapidly and systematically transfers a stack of microplates to and from the instrument’s microplate carrier. Contact BioTek or visit our website to learn more.

  • Page 31: Materials For Conducting Liquid Tests

    Manufacturer part numbers are subject to change. Item Part Number Absorbance Liquid Tests BioTek Wetting Agent Solution BTI #7773002 BioTek QC Check Solution #1 (25 mL) BTI #7120779 BioTek QC Check Solution #1 (125 mL) BTI #7120782 Phosphate-Buffered Saline (PBS) tablets, pH 7.2-7.6 Sigma #P4417 β-NADH Powder (β-Nicotinamide Adenine Dinucleotide,...

  • Page 32: Product Support & Service

    Technical Assistance Center (TAC) If your BioTek product fails to function properly, if you have questions about how to use or maintain our products, or if you need to send an instrument to BioTek for repair or other service, please contact our Technical Assistance Center (“TAC”).

  • Page 33: Installation

    Chapter 2 Installation This chapter includes instructions for unpacking and setting up the Synergy H1 and, as applicable, the gas controller and external dispense module. Instructions are also included for preparing the reader and dispense module for shipment. Product Registration...

  • Page 34: Product Registration

    8 | Chapter 2: Installation Product Registration Please register your products with BioTek to ensure that you receive important information and updates about the products you have purchased. Register online through BioTek’s Customer Resource Center (CRC) at www.biotek.com or by contacting BioTek Customer Care.

  • Page 35: 1: Unpack And Inspect The Synergy H1

    A clean work area is necessary to ensure accurate readings. If you will be installing the BioStack for operation with the Synergy H1, you may wish to seat the instruments in their aligning plates now. Refer to the BioStack Operator’s Manual for more information.

  • Page 36: 3: Remove The Shipping Hardware

    4. Store the shipping hardware with the original packaging for reuse in case you need to ship the instrument. Figure 2-1: Carrier shipping bracket (left), and filter reader shipping bracket BioTek Instruments, Inc.

  • Page 37: 4: Install The Power Supply

    Applies only to models compatible with the BioTek gas controller module If you purchased a gas controller for use with the Synergy H1, you may wish to install it now. Refer to the Gas Controller User Guide for complete instructions.

  • Page 38: 6: Unpack And Inspect The Dispense Module

    Applies only to models equipped with injectors Save all packaging materials. If you need to ship the dispense module to BioTek for repair or replacement, you must use the original materials. Using other forms of commercially available packaging, or failing to follow the repackaging instructions, may void your warranty.

  • Page 39: 7: Install The Dispense Module

    5. Screw the fitting of one inlet tube into the right side of the Syringe 1 valve. 6. Screw one end of one outlet tube into the left side of the Syringe 1 valve. 7. Repeat these steps to attach the inlet and outlet tubing for Syringe 2. Synergy H1 Operator's Manual...
  • Page 40 A magnet helps guide the tips into place and secures them in the reader. Figure 2-3: Injector tip holder in its socket 11. Seat the light shield in the reader's cover and finger-tighten the thumbscrews to secure it. BioTek Instruments, Inc.
  • Page 41 Carefully pull down the bottom of the syringe until it rests inside the hole in the bracket. Pass a thumbscrew up through this hole and thread it into the bottom of the syringe. Hold the syringe to prevent it from rotating while tightening the thumbscrew. Finger-tighten only. Synergy H1 Operator's Manual...

  • Page 42: 8: Connect The Host Computer

    8: Connect the Host Computer The Synergy H1 is equipped with two communication ports, "USB" and "RS232" (serial), located on the back of the reader. Connect one end of the supplied communication cable to the appropriate port on the reader, and the other end to an appropriate port on the host computer.

  • Page 43: 9: Install Gen5 Software

    1. If Gen5 is open, close it now. 2. The power switch is located on the lower-left corner of the front panel; turn on the Synergy H1. The reader performs a System Test. When the test is completed, the reader extends the microplate carrier.

  • Page 44: 12: Verify/Set Dispenser Calibration Values

    Try a different Com port. Did you install the USB driver software? If you remain unable to get Gen5 and the reader to communicate with each other, contact BioTek’s Technical Assistance Center. 12: Verify/Set Dispenser Calibration Values Applies only to models equipped with injectors Confirm that the reader is configured with calibration values for the dispense module.

  • Page 45: 13: Run A System Test

    5. Turn off the incubator. Models with injectors: Keep Gen5 open and proceed to the next section. All other models: The installation and setup process is complete. Close Gen5 and proceed Operational/Performance Qualification on page 21. Synergy H1 Operator's Manual...

  • Page 46: 14: Test The Injection System

    4. Fill the two reagent bottles with distilled or deionized water. Place the bottles in their holders, and place the holders directly in front of the syringes. Insert the inlet tubes into the bottles. 5. In Gen5, select System > Instrument Control > Synergy H1 and click the Prime tab.

  • Page 47: Operational/Performance Qualification

    Operational/Performance Qualification | 21 Operational/Performance Qualification Your Synergy H1 was fully tested at BioTek prior to shipment and should operate properly following the successful completion of the installation and setup procedures described in this chapter. If you suspect that problems occurred during shipment, if you received the reader back...

  • Page 48: Repackaging And Shipping Instructions

    Spilled fluids can contaminate the optics and damage the instrument. The Synergy H1 with all available modules weighs up to 55 pounds (24.95 kg). Use two people when lifting and carrying the instrument. The instrument’s packaging design is subject to change. If the instructions in this section do not apply to the packaging materials you are using, please contact BioTek’s Technical Assistance Center.
  • Page 49 10. 7. Place the accessories in the accessories box and then seal the box with tape. Figure 2-7: Repacking the Synergy H1 accessories box 8. Place the instrument in a plastic bag. 9. Place the instrument in the shipping box with foam corners.
  • Page 50 24 | Chapter 2: Installation 11. When finished, write the SCN number on the outside of the box and ship the box to BioTek. Figure 2-8: Repacking the instrument and accessories box BioTek Instruments, Inc.

  • Page 51: Prepare The Dispense Module For Shipment

    13. Slide the dispenser accessories box into the shipping box. 14. Insert the top foam end cap. Close and seal the outer box with tape. 15. Write the SCN number on the outside of the box. Ship the box to BioTek. Synergy H1 Operator's Manual...
  • Page 52 26 | Chapter 2: Installation Figure 2-9: Packing the dispense module accessories BioTek Instruments, Inc.
  • Page 53 Repackaging and Shipping Instructions | 27 Figure 2-10: Packing the dispense module Synergy H1 Operator's Manual...
  • Page 54 28 | Chapter 2: Installation BioTek Instruments, Inc.

  • Page 55: Getting Started

    Chapter 3 Getting Started This chapter describes some of the Synergy H1's external and internal components, and provides an introduction to using Gen5 software to control the instrument and, if equipped, dispense module. Modular Design External Components Internal Components Filter Cube...

  • Page 56: Modular Design

    30 | Chapter 3: Getting Started Modular Design The Synergy H1 is a multi-mode microplate reader, with a design that allows you to initially purchase only the capabilities you need and then upgrade later as your requirements expand. Please contact BioTek Customer Care to learn more about your upgrade options.

  • Page 57: External Components

    External Components | 31 External Components Figure 3-1: Synergy H1, front view Internal Components As discussed on page 30, not all Synergy H1 models contain these components. Component Description Filter Cube The filter cube can contain excitation and emission filters, mirrors, and polarizing filters.

  • Page 58: Filter Cube

    32 | Chapter 3: Getting Started Filter Cube Most Synergy H1 models are equipped with a filter cube that contains excitation and emission filters, mirrors, and, if required, polarizing filters. Excitation and emission filters are used for obtaining fluorescence and luminescence measurements.

  • Page 59: Injection System

    Inlet tubes transport fluid from the supply bottles to the syringes. These tubes are short pieces of opaque PTFE (Teflon) tubing connected to stainless-steel probes on one end and threaded fittings on the other end. Valves switch the syringe flow from the inlet tubes to the outlet tubes. Synergy H1 Operator's Manual...

  • Page 60: Priming The Injection System

    For information on the materials used in the injection system, refer to Injection System - Chemical Compatibility Technical Note on the USB flash drive supplied with the Synergy H1. Priming the Injection System Before running a Dispense assay, use Gen5 to prime the system with the reagent or dispensing fluid.

  • Page 61: Gen5 Software

    Gen5 Software | 35 Gen5 Software Gen5 supports all Synergy H1 models. Use Gen5 to control the reader, the dispense module (if equipped), and the BioStack (if equipped); perform data reduction and analysis on the measurement values; print or export results; and more. This section provides brief instructions for working with Gen5 to create protocols and experiments and read plates.

  • Page 62: Protocols And Experiments

    Open the Data Reduction dialog to add data reduction steps. Categories include Transformations, Well Analysis, Curve Analysis, and Qualitative Analysis. Create a report or export template via the Report/Export Builders. 7. Select File > Save and give the protocol an identifying name. BioTek Instruments, Inc.

  • Page 63: Dispense Module Control

    2. Fill the supply bottle with a sufficient volume of the fluid to be used for the prime and the assay. Insert the appropriate inlet tube into the bottle. 3. Select System > Instrument Control > Synergy H1 and click the Prime tab.

  • Page 64: Plate Shaking Options

    Purge to start the process. Plate Shaking Options The Synergy H1 supports multiple plate shaking options, as described below. Shaking is controlled using Gen5 by adding a Shake step to a protocol's procedure. Figure 3-3: Gen5 Shake Step options Mode...

  • Page 65: Recommendations For Optimum Performance

    Design your Gen5 protocol in advance as well, to ensure that the intended reading parameters are used and to avoid any last-minute corrections. Although the Synergy H1 supports standard flat, U-bottom, and V-bottom microplates, the reader achieves optimum performance with flat-bottomed wells when running in Absorbance mode.

  • Page 66: Luminescence Measurements

    Cluster your sample wells rather than spacing them throughout the plate. Place your sample wells in the center of the plate. This placement may lead to less evaporation than if you place the samples in wells on the edge of the plate. BioTek Instruments, Inc.

  • Page 67: Kinetic Assays Using The Continuous Shake Feature

    Recommendations for Optimum Performance | 41 Kinetic Assays Using the Continuous Shake Feature This recommendation applies only to Synergy H1 basecode software versions lower than 2.00. A Gen5 experiment that specifies the following parameters may not run successfully on the...
  • Page 68 42 | Chapter 3: Getting Started BioTek Instruments, Inc.

  • Page 69: Filters And Mirrors

    Chapter 4 Filters and Mirrors Getting Started chapter provided an overview of the filters and mirrors installed in some Synergy H1 models. This chapter provides more detailed information on working with these components. Filter Cube Overview Removing a Filter Cube...

  • Page 70: Filter Cube Overview

    44 | Chapter 4: Filters and Mirrors Filter Cube Overview Most Synergy H1 models are equipped with a filter cube that contains excitation filters, emission filters, and mirrors for use when taking fluorescence and luminescence measurements. Each filter cube contains two filter sets, each of which contains one excitation filter, one emission filter, and one mirror.
  • Page 71 If the mirror is positioned incorrectly, your measurement data may be inaccurate. For Synergy H1 models with FP capability, the cube is equipped with up to four polarizers of the following types: Excitation polarizer (visible-range or UV-range)

  • Page 72: Removing A Filter Cube

    2. Open the front access door and locate the filter cube chamber. 3. With the filter cube properly oriented (the non-labeled side entering the chamber), gently slide the cube into the chamber. You will feel a magnet engage the cube. See the photos on the next page. BioTek Instruments, Inc.

  • Page 73: Configuring The System For Luminescence Measurements

    This name will become available for selection in the protocol/experiment procedure. 3. If applicable, check the Fluorescence Polarization Cube box. 4. Enter a name for Filter Set 1. 5. Define the excitation and emission filters: Synergy H1 Operator's Manual...
  • Page 74 Filter Set 1 for fluorescence polarization. 7. Define Filter Set 2, if necessary. 8. Click to return to the Filter Cubes dialog. Click the Help button to learn about the Read Plate Prompt options. BioTek Instruments, Inc.

  • Page 75: Handling Filters And Mirrors

    44. If you run different types of fluorescence and luminescence assays, you can replace the entire filter cube with a different one; this is the BioTek-recommended option. Alternatively, you can install different filters or mirrors in the cube; this section describes how to do this.
  • Page 76 Use several layers of lens paper and your finger or a cotton swab to remove and replace filters and clips. Using a sharp instrument, such as a flat screwdriver, will scratch the filter surface and make it unusable. BioTek Instruments, Inc.
  • Page 77 It is critical that the Gen5 Filter Cube table reflect the actual location and characteristics of the filters and mirrors in the installed filter cube. See instructions on page 35. Synergy H1 Operator's Manual...

  • Page 78: Clean The Filters And Mirrors

    Filters and Mirrors Available from BioTek Bandpass filters, plugs, retainer clips, mirrors, empty filter cubes, and other supplies are available for purchase from BioTek. Visit www.biotek.com and go to the Accessories page to see the most up-to-date list. Contact BioTek Customer Care with any questions.

  • Page 79: Preventive Maintenance

    Chapter 5 Preventive Maintenance This chapter provides instructions for maintaining the Synergy H1 and external dispense module (if used) in top condition, to ensure that they continue to perform to specification. Overview Recommended Maintenance Schedule Warnings and Precautions Clean Exposed Surfaces...

  • Page 80: Overview

    54 | Chapter 5: Preventive Maintenance Overview A general Preventive Maintenance (PM) regimen for the Synergy H1 includes periodically cleaning all exposed surfaces and inspecting/cleaning the excitation and emission filters and mirrors (if equipped). For models with the external dispense module, additional tasks include flushing/purging the fluid path and cleaning the tip prime trough, priming plate, supply bottles, internal dispense tubing, and injector heads.

  • Page 81: Recommended Maintenance Schedule

    Recommended Maintenance Schedule | 55 Recommended Maintenance Schedule The table below contains the recommended Preventive Maintenance tasks for Synergy H1 and the frequency with which each task should be performed. The risk and performance factors associated with your assays may require that some or all of the Preventive Maintenance procedures be performed more frequently than shown here.

  • Page 82: Warnings And Precautions

    Do not allow water or other cleaning solution to run into the interior of the instrument. If this happens, contact BioTek’s Technical Assistance Center. Important! Do not apply lubricants to the microplate carrier or carrier track.

  • Page 83: Clean Exposed Surfaces

    Models with injectors: If the tip priming trough overflows or other spills occur inside the instrument, wipe the carrier and the surface beneath the carrier with a dry cotton cloth. The internal chamber and probes are not customer-accessible. If overflow is significant, contact BioTek’s Technical Assistance Center. Synergy H1 Operator's Manual...

  • Page 84: Inspect/Clean Excitation And Emission Filters

    58 | Chapter 5: Preventive Maintenance Inspect/Clean Excitation and Emission Filters Applies only to models with fluorescence and/or luminescence capability BioTek recommends inspecting the filters for dust and other debris every three months. To clean them, you will need: Isopropyl, ethyl, or methyl alcohol...

  • Page 85: Inspect/Clean Mirrors

    Inspect/Clean Mirrors Applies only to models with fluorescence and/or luminescence capability BioTek recommends inspecting/cleaning the mirrors and polarizing filters (if equipped) annually, especially if the filter cube has been opened or changed. The mirrors (especially the dichroic) and polarizing filters can be easily damaged.

  • Page 86: Procedure

    See Figure 4-3 on page 46. 10. Place the filter cube top back onto the cube and replace the screw and washer. 11. Reinstall the filter cube in the reader. BioTek Instruments, Inc.

  • Page 87: Flush/Purge Fluid Path

    After purging the system, you may wish to run a quick Dispense protocol to visually verify the dispense accuracy (see the next section) or the more thorough Dispense Accuracy and Precision Tests (see Injection System Tests starting on page 134). Synergy H1 Operator's Manual...

  • Page 88: Run A Dispense Protocol (Optional)

    If the well volume appears to be unevenly distributed, clean the internal dispense tubes and injectors as described in Clean the Dispense Tubes and Injectors starting on page and run the protocol again. BioTek Instruments, Inc.

  • Page 89: Empty/Clean The Tip Priming Trough

    Required Materials Protective gloves and safety glasses Mild detergent Clean, lint-free cotton cloths Deionized or distilled water Stylus (affixed to the rear of the dispense module or reader) (BTI #2872304) Synergy H1 Operator's Manual...

  • Page 90: Remove The Dispense Tubes And Injector Tip Holder

    Stream water through the pipe to be sure it is clean. If the water does not stream out, try soaking in hot, soapy water and then reinserting the stylus. BioTek Instruments, Inc.

  • Page 91: As-Needed Maintenance

    Chapter 6 As-Needed Maintenance This chapter contains maintenance and component-replacement procedures that need to be performed only occasionally. Decontamination Required Materials Procedure for Models without the Dispense Module Procedure for Models with the Dispense Module Dispense Module, Syringe Replacement Syringe Maintenance Position Replace the Syringe...

  • Page 92: Decontamination

    Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the biohazards they handle.

  • Page 93: Procedure For Models Without The Dispense Module

    Do not immerse the instrument, spray it with liquid, or use a dripping- wet cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department. Turn off and unplug the instrument for all decontamination and cleaning operations.

  • Page 94: Procedure For Models With The Dispense Module

    61) and detach the outlet tubes from the instrument. If it is not installed, attach only the dispense module’s communication cable to the instrument. Remove the supply bottles and their holders. 10. Perform the decontamination procedures described below. BioTek Instruments, Inc.

  • Page 95: Decontaminate The Fluid Lines

    6. Pause for 10 minutes and then run one prime cycle with 5000 µL. This delay will allow any residual sodium hypochlorite to diffuse into the solution and be flushed out with the next prime. 7. Empty the beaker containing the outlet tubes. 8. Wipe all surfaces with deionized water. Synergy H1 Operator's Manual...

  • Page 96: Clean The Tubing And Injectors

    All surfaces of the plate carrier The exposed surfaces of the dispense module, including the syringe valves 6. Remove the tubing and the syringes from the dispense module and soak them in the bleach or alcohol solution. Wait for 20 minutes. BioTek Instruments, Inc.

  • Page 97: Dispense Module, Syringe Replacement

    To change a syringe, first use Gen5 to put the syringe in its maintenance position. Syringe Maintenance Position Do not change the syringe position or calibrate the dispensers unless instructed to do so by BioTek as part of installation, upgrade, or maintenance. Gen5 provides access to syringe setup functions for maintenance and calibration purposes.

  • Page 98: Replace The Syringe

    6. Pass the thumbscrew (used to hold the old syringe) up through this hole and thread it into the bottom of the syringe. Hold the syringe from rotating while tightening the thumbscrew. Finger-tighten only. 7. In Gen5, select System > Instrument Control > Synergy 8. Click the Prime tab and click Initialize. BioTek Instruments, Inc.

  • Page 99: Instrument Qualification Process

    Instrument Qualification Process This chapter describes the tests that BioTek Instruments, Inc. has developed for complete qualification of all models of the Synergy H1. This chapter introduces the various test methods, describes the materials and relevant Gen5 protocols used to execute the tests, explains how to analyze test results, and provides troubleshooting tips in the event of a failure.

  • Page 100: Instrument System Test

    74 | Chapter 7: Instrument Qualification Process Instrument System Test Each time the Synergy H1 is turned on, it automatically performs a series of tests on the reader’s motors, lamp(s), the PMT, and various sub-systems. The duration of this “system test”...

  • Page 101: Absorbance Testing

    Before the Absorbance Plate Test can be performed, the OD Standard values and Expected Peak/Test Range combinations must be entered into Gen5. Enter and save these values once initially, and then update them annually when the test plate is recertified by BioTek. Test Methods The Absorbance Plate Test is conducted using Gen5 software (System >...

  • Page 102: Sample Test Report

    Refer to Sample Reports on page to see a sample Absorbance Plate Test Report for Synergy H1. Troubleshooting If a test fails, try the troubleshooting tips below. If the test continues to fail, contact BioTek TAC. Important! Do not remove filters from the Absorbance Test Plate.

  • Page 103: Alignment Test

    Check the microplate carrier to ensure it is clear of debris. Absorbance Liquid Tests BioTek Instruments, Inc. has developed a series of liquid test procedures for testing your reader's absorbance system. Test Methods Absorbance Liquid Test 1 confirms repeatability and alignment of the reader when a solution is used in the microplate.

  • Page 104: Gen5 Protocol Parameters

    340 nm. (Alternatively, the BioTek 340 nm Absorbance Test Plate may be used; see page 75.) This test is optional since the Synergy H1 has good “front- end” linearity throughout the specified wavelength range. While the absolute values of the OD cannot be determined by this test, the results will indicate if there is adequate repeatable absorbance and a linear slope.
  • Page 105 Absorbance Testing | 79 Synergy H1 Abs Test 2.prt Parameter Setting Plate Type 96 WELL PLATE Shake Step Linear, 4 minutes, default frequency Two Read Steps Kinetic loop (one per Read step) Set a Run Time/Interval combination to read the...

  • Page 106: Results Analysis

    Calculate the mean absorbance for each well, and average the means for each concentration. Perform a regression analysis on the data to determine if there is adequate linearity. Since it is somewhat difficult to achieve high pipetting accuracy when BioTek Instruments, Inc.

  • Page 107: Absorbance Liquid Test 3

    Example: Five readings in well A1 of 0.802, 0.802, 0.799, 0.798, and 0.801 result in a Mean of 0.8004 and a Standard Deviation of 0.0018. The Mean multiplied by 1.0% (0.8004 * 0.010) equals 0.008, and when added to 0.005 equals 0.013; this is Synergy H1 Operator's Manual...

  • Page 108: Troubleshooting

    Liquid Test 1 can fail due to the meniscus effect, which can cause readings to decrease over time. If you suspect this may be the case, include a shake step between the read steps in the protocol. BioTek Instruments, Inc.

  • Page 109: Luminescence Testing

    For models with luminescence capability, BioTek uses the Harta Luminometer Reference Microplate to test the luminescence system. The test plate is LED-based and NIST- traceable. Contact BioTek to purchase a plate (BTI #8030015; includes microplate carrier adapters) or visit www.HartaInstruments.com to learn more.
  • Page 110 Optics Type Filters Step Label Battery check Read wells A7–A8 Filter Set 1 (filter cube) Excitation Plug Emission Hole Gain Integration Time 0:01.00 MM:SS.ss Delay After Plate Movement 350 msec Dynamic Range Extended Read Height 10.00 mm BioTek Instruments, Inc.

  • Page 111: Results Analysis

    Convert it to attomoles: (A2 NIST measurement * 0.02884) 3. Calculate the signal-to-noise ratio: (A2–Mean of the buffer cells)/(3 * Standard deviation of buffer cells) 4. Calculate the detection limit: A2 NIST measurement in attomoles/signal-to-noise ratio Synergy H1 Operator's Manual...

  • Page 112: Pass/Fail Criteria

    #49721=red-shifted PMT), please contact BioTek TAC. Troubleshooting If a test fails, try the suggestions below. If a test continues to fail, contact BioTek's Technical Assistance Center (TAC). Ensure that the reading is performed through a hole in the filter cube, not through a glass filter.

  • Page 113: Fluorescence Testing

    The Fluorescence Test Plate simplifies the process for conducting fluorescence intensity, fluorescence polarization, and time-resolved fluorescence qualification tests on the Synergy H1. The test plate is solid and therefore immune to the pipetting errors, evaporation issues, and costs experienced with conventional Liquid Tests.

  • Page 114: Fluorescence Liquid Tests

    Gen5 protocols used with liquid testing. It is possible that your tests will require modifications to some of these parameters, such as the Plate Type. i The Plate Type setting in each Gen5 protocol should match the actual plate in use. BioTek Instruments, Inc.
  • Page 115 C9, D9, E9 Filter Set 1 ("Green") Excitation 485/20 nm Emission 528/20 nm Optics Position Top 510 nm Gain Auto, Use first filter set gain from FIRST read step Read Speed Normal Delay after plate movement 350 msec Synergy H1 Operator's Manual...
  • Page 116 Emission 528/20 nm Optics Position Top 510 nm Gain Auto, Scale to High Wells, C1, 50000 Read Speed Normal Delay after plate movement 350 msec Measurements per data point Lamp Energy Low (faster) Read Height 7.00 mm BioTek Instruments, Inc.
  • Page 117 Fluorescence Testing | 91 Synergy H1 M_FI_T_SF.prt and Synergy H1 M_FI_B_SF.prt Parameter Setting Plate Type "Costar 96 black opaque" (Top) "Greiner SensoPlate" (#655892) (Bottom) Read Step 1 Kinetic loop Run time 0:01:00, Interval 0:00:04 (16 reads) Detection Method Fluorescence Read Type...
  • Page 118 1 ("Green") Excitation 485 nm Emission 528 nm Optics Position Top (or Bottom) Gain Auto, Scale to High Wells, C1, 50000 Read Speed Normal Delay after plate movement 350 msec Measurements per data point Lamp Energy Low (faster) BioTek Instruments, Inc.
  • Page 119 Linear, 30 seconds, default frequency Read Step 1 Kinetic loop Run time 0:00:30, Interval 0:00:02 (16 reads) Detection Method Time-resolved fluorescence Read Type Endpoint / Kinetic Optics Type Filters Step Label "Sensitivity Read" Read well Filter Set 1 ("TRF") Synergy H1 Operator's Manual...
  • Page 120 Auto, Use first filter set sensitivity from FIRST Read Step Read Speed Normal Delay after plate movement 350 msec Measurements per data point Lamp Energy Low (faster) Delay before collecting data 300 µsec Data collection time 1000 µsec Read Height 7.00 mm BioTek Instruments, Inc.
  • Page 121 C9, D9, E9 Filter Set 1 ("Blue") Excitation 360/40 nm Emission 460/40 nm Optics Position Top 400 nm Gain Auto, Use first filter set gain from FIRST Read Step Read Speed Normal Delay after plate movement 350 msec Synergy H1 Operator's Manual...
  • Page 122 Delay after plate movement 350 msec Measurements per data point Lamp Energy Low (faster) Read Height 7.00 mm Synergy H1 M_FI_T_MUB.prt Parameter Setting Plate Type "Costar 96 black opaque" (#3915) Read Step 1 Kinetic loop Run time 0:01:00, Interval 0:00:04 (16 reads)
  • Page 123 Read Height 7.00 mm Read Step 3 Detection Method Fluorescence intensity Read Type Endpoint / Kinetic Optics Type Filters Step Label "Linearity Read" Read well C1-F5 Filter Set Excitation 360 nm Emission 460 nm Optics Position Synergy H1 Operator's Manual...

  • Page 124: Results Analysis

    Filter-based system, top optics: The Detection Limit must be <=10 pM (2 pg/mL) to pass. Monochromator-based system, top/bottom optics: The Detection Limit must be <=20 pM (3.76 pg/mL) to pass. Methylumbelliferone: Using the known concentration value of MUB and the calculated SNR: 17.6/SNR BioTek Instruments, Inc.

  • Page 125: Linearity Test

    Calculate the Signal for each LPR well: Subtract the Mean Blank from its measurement value. 2. Using the raw data from the Perpendicular read: Calculate the Mean Blank (wells A6–H6). Calculate the Signal for each HPR well: Subtract the Mean Blank from its measurement value. Synergy H1 Operator's Manual...

  • Page 126: Time-Resolved Fluorescence (Trf) Test

    4. Calculate the Signal-to-Noise Ratio (SNR) using the Mean Eu Concentration and Buffer Median STD with its corresponding Buffer Mean: (Eu Mean - Buffer Mean)/(3 * Buffer STD) 5. Calculate the Detection Limit, in fM: 20000/SNR The Detection Limit must be <=250 fM to pass. BioTek Instruments, Inc.

  • Page 127: Troubleshooting

    Does the Plate Type setting in the Gen5 protocol match the plate you used? For injector models, spilled fluid inside the reader may be fluorescing, which can corrupt your test results. If you suspect this is a problem, contact BioTek TAC for assistance.

  • Page 128: Injection System Testing

    2.0% for 80 μL, 5.0% for 20 μL, and 20.0% for 5 μL. The test uses a green dye test solution (available for purchase from BioTek, see page 5) and one 96-well microplate per injector to test the three different volumes. The balance is tared with the empty plate and the 80 μL dispense is performed for columns 1–4.
  • Page 129 Wavelengths, 2: 630 nm, 750 nm Speed: Normal Data Reduction Define two Delta OD transformations: 405–750 nm for the 80 uL Read step, columns 1–4 630–750 nm for the 20 and 5 uL Read step, columns 5-12 Synergy H1 Operator's Manual...
  • Page 130 104 | Chapter 7: Instrument Qualification Process Synergy H1 Disp 1 Test No Read.prt and Synergy H1 Disp 2 Test No Read.prt (for use with models without Absorbance capability) Parameter Setting Plate Type 96 WELL PLATE Dispense Step Dispenser <1 or 2>...

  • Page 131: Results Analysis

    If any tests fail, prime the fluid lines and rerun the tests. If the tests fail again, the injectors may require cleaning; see Clean the Dispense Tubes and Injectors on page 63. If tests continue to fail, contact BioTek TAC. Synergy H1 Operator's Manual...
  • Page 132 106 | Chapter 7: Instrument Qualification Process BioTek Instruments, Inc.

  • Page 133: Instrument Qualification Procedures

    Chapter 8 Instrument Qualification Procedures This chapter contains the step-by-step procedures for verifying that the Synergy H1 and its various sub-systems are performing to specification. Instrument Qualification Process starting on page introduces the various test methods, describes the materials and relevant Gen5 protocols used to execute the tests, explains how to analyze test results, and provides troubleshooting tips in the event of a failure.

  • Page 134: Overview

    Performance Qualification, and Preventive Maintenance. Contact your BioTek dealer for more information. If the gas controller module is used with the Synergy H1, refer to the Gas Controller User Guide (BTI #1211000) or Gas Controller Product Qualification Package (BTI #1210512) for qualification procedures.

  • Page 135: Iq/Oq/Pq Description

    These tests should be performed routinely; the recommended interval is monthly or quarterly, depending on the test. This frequency may be adjusted depending on the trends observed over time. The successful completion of the PQ procedure confirms that the equipment is performing consistently under normal operating conditions. Synergy H1 Operator's Manual...

  • Page 136: Recommended Qualification Schedule

    110 | Chapter 8: Instrument Qualification Procedures Recommended Qualification Schedule The schedule below defines BioTek-recommended intervals for qualifying a Synergy H1 used two to five days a week. The actual frequency, however, may be adjusted depending on your usage of the instrument and its various modules. This schedule assumes the reader...

  • Page 137: System Test

    If the test continues to fail, or if the cause is not something you can fix, contact BioTek TAC. 4. If required, print, sign, and date the report, and store it with your test documentation.

  • Page 138: Absorbance Plate Tests

    Before an Absorbance Test Plate can be used for qualification, you must enter information from its Calibration Certificate into Gen5. Perform these steps initially, and then repeat them annually after the test plate is recertified by BioTek: 1. Obtain the current Test Plate Calibration Certificate.

  • Page 139: Test Procedure

    If the C6 filter is Erbium or Holmium glass, the certificate contains two Spectral Bandpass tables. Synergy H1 has a bandpass wider than 5 nm for wavelengths above 285 nm and smaller than 4 nm for 230–285 nm; we recommend you use the expected peak values in the 5.0 nm table.

  • Page 140: Absorbance Liquid Tests

    5-mL Class A volumetric pipette 100-mL volumetric flask 1. Pipette a 5-mL aliquot of BioTek QC Check Solution No. 1 into a 100-mL volumetric flask. 2. Add 95 mL of DI water; cap and shake well. The solution should measure approximately 2.000 OD when using 200 μL in a flat-bottom microwell.

  • Page 141: Solution B

    2. Pipette 200 μL/well of the stock solution into column 1. 3. Pipette 200 μL/well of the diluted solution into column 2. 4. Create a Gen5 experiment based on the Synergy H1 Abs Test 1 protocol and read the plate. When prompted, rotate the plate 180 degrees and continue.

  • Page 142: Absorbance Liquid Test 2

    (Tube 2 into wells A2 to H2, Tube 3 into wells A3 to H3, and so on). 4. Create a Gen5 experiment based on the Synergy H1 Abs Test 2 protocol and read BioTek Instruments, Inc.

  • Page 143: Absorbance Liquid Test 3

    Absorbance Liquid Test 3 is provided for sites requiring proof of linearity at 340 nm. This test is optional because the Synergy H1 has good "front end" linearity throughout its wavelength range. As an alternative, the 340 nm Absorbance Test Plate (BTI #7260551) may be used for this test.

  • Page 144: Test Procedure

    150 μL of the 75% Test Solution into all wells of columns 3 and 4 150 μL of the 50% Test Solution into all wells of column 5 and 6 4. Create a Gen5 experiment based on the Synergy H1 Abs Test 3 protocol and read the plate.

  • Page 145: Luminescence Test

    3. Place the adapter on the reader's microplate carrier and then place the Harta reference plate on top of the adapter. 4. In Gen5, create an experiment based on the Synergy H1 F-Lum-Test (filter-based) protocol and initiate a plate read.

  • Page 146: Fluorescence Plate Tests

    Microsoft Excel .xls file. The worksheet(s) within that file calculate results and determine pass or fail. i For use with the Synergy H1, identify the reader-specific Gen5 protocols on the USB flash drive that came with the test plate. Use only those protocols that apply to your reader model and your organization's qualification requirements.

  • Page 147: Fluorescence Liquid Tests

    Manufacturer part numbers are subject to change. All tests: Deionized or distilled water Various beakers, graduated cylinders, and pipettes 95% ethanol (for cleaning clear-bottom plates) Aluminum foil (Optional, but recommended) 0.45-micron filter (Optional) Black polyethylene bag(s) to temporarily store plate(s) Synergy H1 Operator's Manual...
  • Page 148 Excitation: Band Pass, 360/40 Mirror: Dichroic, Top 400 nm (320/390, 410/800) Emission: Band Pass, 620/40 Filter Set Name FP (enable 'Fluorescence Polarization Cube') Excitation: Band Pass, 485/20 Mirror: Dichroic, Top 510 nm (440/505, 515/640) Emission: Band Pass, 528/20 BioTek Instruments, Inc.

  • Page 149: Corners/Sensitivity/Linearity (Fi) Tests

    The FP Test may be conducted using the same plate as for the Top Corners/Sensitivity/Linearity (FI) Tests. The recommended test solutions are available from Invitrogen Corporation in their “FP One-Step Reference Kit” (#P3088) or BioTek (#7160014; see page 5). This kit includes: (Green) Polarization Reference Buffer, 15 mL...

  • Page 150: Time-Resolved Fluorescence (Trf) Test

    Corners/Sensitivity/Linearity (FI) Tests: If using BioTek’s sodium fluorescein powder (BTI #98155), be sure to hold the vial upright and open it carefully; the material may be concentrated at the top. If a centrifuge is available, spin down the tube before opening.

  • Page 151: Fluorescence Polarization (Fp) Test

    Fluorescence Polarization (FP) Test: As described in the Materials section, the recommended test solutions are available from Invitrogen Corporation (or BioTek). They do not require additional preparation. Time-Resolved Fluorescence (TRF) Test: As described in the Materials section, the recommended test solutions are available from Invitrogen Corporation (or BioTek).

  • Page 152: Test Procedure

    Create an experiment based on Synergy H1_TRF.prt and read the plate. 6. Save the experiments. Refer to the instructions starting on page to perform calculations and determine pass/fail. Troubleshooting tips are provided on page 101. Test descriptions are provided on page 88. BioTek Instruments, Inc.

  • Page 153: Pipette Maps

    Do not discard the tips. Aspirate 150 μL from column 4 and dispense into column 5. Mix the wells using the pipette. Do not discard the tips. Aspirate 150 μL from column 5. Discard the solution and the tips. Synergy H1 Operator's Manual...

  • Page 154: Fluorescence Polarization (Fp) Test

    Pipette 200 μL of the (green) polarization buffer (BUF) into wells A6–H6. Pipette 200 μL of the green high polarization reference (HPR) into wells A7–B7. Pipette 200 μL of the green low polarization reference (LPR) into wells A8–H8. BioTek Instruments, Inc.

  • Page 155: Time-Resolved Fluorescence (Trf) Test

    If you have not already done so, shake the vial of 20 pM europium suspension vigorously for 30 seconds prior to pipetting. Alternatively, sonicate or vortex the vial. Pipette 200 μL of the 20 pM europium suspension (Eu) into well A8. Synergy H1 Operator's Manual...

  • Page 156: Alternate/Supplemental Tests Using Methylumbelliferone (Mub)

    (Optional) Black polyethylene bag(s) to temporarily store plate(s) Gen5 protocols listed below (as applicable for your reader model) and described in detail under Gen5 Protocol Parameters starting on page 88: Synergy H1_FI_T_MUB.prt (filter-based system) Synergy H1_M_FI_T_MUB.prt (monochromator-based system) BioTek Instruments, Inc.

  • Page 157: Test Solutions

    0.1 mL of 176 µg/mL solution 9.9 mL of CBB 1.76 µg/mL 0.5 mL of 1.76 µg/mL solution 4.5 mL of CBB 176 ng/mL 1 mL of 176 ng/mL solution 9 mL of CBB 17.6 ng/mL (100 nM) Synergy H1 Operator's Manual...

  • Page 158: Test Procedure

    Create an experiment based on Synergy H1_M_FI_T_MUB.prt and read the plate. 4. Save the experiments. Refer to the instructions starting on page to perform calculations and determine pass/fail. Troubleshooting tips are provided on page 101. Test descriptions are provided on page 88. BioTek Instruments, Inc.

  • Page 159: Pipette Map

    12.5 nM 6.25 nM 50 nM 25 nM 12.5 nM 6.25 nM 100 nM 100 nM 100 nM 50 nM 25 nM 12.5 nM 6.25 nM 100 nM 50 nM 25 nM 12.5 nM 6.25 nM Synergy H1 Operator's Manual...

  • Page 160: Injection System Tests

    ±1.0% ±0.010 OD or better and a repeatability specification of ±1.0% ±0.005 OD or better. The Synergy H1 may be used if it is equipped with Absorbance capabilities and has passed the Absorbance Plate Test or Absorbance Liquid Test 1.

  • Page 161: Alternate Test Solutions

    Injection System Tests | 135 Alternate Test Solutions If you do not have BioTek’s Green Test Dye Solution (PN 7773003), prepare a dye solution using one of the following methods: 80 μL of test solution with 150 μL of deionized water should read between 1.300 and 1.700 OD at 405/750 nm.
  • Page 162 9. When the experiment is complete, save the file with an identifying name. 10. Remove the plate from the carrier and set it aside. 11. Repeat the procedure using the Synergy H1 Disp 2 Test protocol and a new microplate.

  • Page 163: Test Procedure For Models Without Absorbance Capability

    2000 μL of the solution. When finished, remove the priming plate from the carrier. 4. Create an experiment based on the Synergy H1 Disp 1 Test No Read protocol. 5. Place a new 96-well microplate on the balance and tare the balance.
  • Page 164 12. When the experiment is complete, save the file with an identifying name. 13. Remove the plate from the carrier and set it aside. 14. Repeat the procedure using the Synergy H1 Disp 2 Test No Read protocol and a new microplate.

  • Page 167: Specifications

    Appendix A Specifications This appendix contains BioTek's published specifications for the Synergy General Specifications Absorbance Specifications Fluorescence Specifications Luminescence Specifications Dispense/Read Specifications...

  • Page 168: General Specifications

    140 | Appendix A: Specifications General Specifications Microplates The Synergy H1 accommodates standard 6-, 12-, 24-, 48-, 96-, and 384-well microplates with 128 x 86 mm geometry, and the BioTek Take3 and Take3Trio Micro-Volume Plates. Maximum Plate Height: Absorbance mode: plates up to 0.8" (20.30 mm) high Fluorescence, monochromator-based mode: plates up to 0.89"...

  • Page 169: Optics

    0–2 OD: ±1% ±0.010 OD, delay after plate movement=100 ms 2–2.5 OD: ±3% ±0.010 OD, delay after plate movement=100 ms 384-well plate, normal read speed 0–2 OD: ±2% ±0.010 OD, delay after plate movement=100 ms 2–2.5 OD: ±5% ±0.010 OD, delay after plate movement=100 ms Synergy H1 Operator's Manual...

  • Page 170: Repeatability

    96-well and 384-well plate, sweep read speed 0–1 OD: ±2% ±0.010 OD Fluorescence Specifications The Synergy H1 measures fluorescence from the top and bottom with monochromators, and from the top with filters. The following sets of requirements apply to 96-well plates. Monochromator-Based Fluorescence Excitation Range 250–700 nm (with low-noise PMT)

  • Page 171: Filter-Based Fluorescence

    Excitation range 330–700 nm (UV-transparent polarizing filter) Emission range 400–700 nm Luminescence Specifications ≤ 75 amol/well DL ATP in a 96-well plate (low-noise PMT), 30 amol typical ≤ 500 amol/well DL ATP in a 96-well plate (red-shifted PMT) Synergy H1 Operator's Manual...
  • Page 172 5–1000 µL with a 5–20 µL tip prime Accuracy ±1 µL or 2.0%, whichever is greater Precision ≤2.0% for volumes of 50–200 µL ≤4.0% for volumes of 25–49 µL ≤7.0% for volumes of 10–24 µL ≤10.0% for volumes of 5–9 µL BioTek Instruments, Inc.

  • Page 173: Error Codes

    Appendix B Error Codes This appendix lists and describes Synergy H1 error codes that may appear in Gen5. Overview Error Codes...

  • Page 174: Overview

    146 | Appendix B: Error Codes Overview When a problem occurs during operation with the Synergy H1, an error code appears in Gen5. Error codes typically contain four characters, such as “4168,” and in most cases are accompanied by descriptive text, such as “PMT overload error.” With many errors, the instrument will beep repeatedly;...
  • Page 175 If an error code appears in Gen5, look for it here. If you find the code, follow the suggestions provided for solving the problem. If you cannot find the code or if you are unable to solve the problem, please contact BioTek’s Technical Assistance Center. The Gen5 Help system also provides troubleshooting tips.
  • Page 176 Try lowering the gain/sensitivity in your Read step(s). For models with the dispense module, the internal chamber may require cleaning (contact BioTek TAC). If a 4E18 error is detected during monochromator-based fluorescence, the luminescence probe may be picking up stray light.
  • Page 177 This error indicates that an axis failed a previous verify function and now needs to be homed. Verify that the shipping brackets have been removed. Check for any obstructions that may prevent the carrier, syringes, or filter cube from moving normally. Restart the reader. Synergy H1 Operator's Manual...
  • Page 178 This error can also occur if the carrier is inside and the newly- defined plate height is different from the most-recently specified plate height. To resolve this error, eject the carrier prior to running the experiment. BioTek Instruments, Inc.

  • Page 179: Instrument Dimensions For Robotic Interface

    Interface This appendix shows the location of the microplate carrier in reference to the exterior surfaces of the Synergy H1 and the mounting holes on the bottom. Use the illustrations to facilitate system setup with a robotic instrument, such as the BioStack Microplate Stacker.
  • Page 180 152 | Appendix C: Instrument Dimensions for Robotic Interface Figure C-1: Top view BioTek Instruments, Inc.
  • Page 181 | 153 Figure C-2: Bottom view The arrows point to special mounting holes for alignment caps for operation with the BioStack; note that the model shown is not gas-ready. Synergy H1 Operator's Manual...
  • Page 182 154 | Appendix C: Instrument Dimensions for Robotic Interface Figure C-3: Left side view BioStack users: Special alignment hardware is included in the BioStack’s alignment kit for correct positioning with the Synergy H1. Refer to the Installation chapter in the BioStack Operator’s Manual for instructions. BioTek Instruments, Inc.

  • Page 183: Sample Reports

    Appendix D Sample Reports This appendix contains sample System Test and Absorbance Plate Test reports for the Synergy H1.
  • Page 184 Gen5 System Test Report Reader: Synergy H1 (Serial Number: 1506126) Basecode: P/N 8040200 (v2.00) Gen5 Version: 2.07.17 Date and Time: 6/12/2015 2:28:12 PM User: 4205 Company: BioTek Comments: Test Results SYSTEM TEST PASS Operator ID:______________________________________________________________ Notes:____________________________________________________________________ __________________________________________________________________________ SYSTEM SELF TEST 8040200 Version 2.00...
  • Page 185 #6:999 1.88 Light 12410 39607 Dark 10613 10653 Delta 1797 28954 Noise Test Meas Gain 1.00 10592 10629 10591 10628 Delta FLUORESCENCE/LUMINESCENCE Monochromator PCB Bias current offset -0.8 counts PASS Offset voltage 1683 counts PASS 750V measurement 28.4 counts PASS 750V noise 4 counts 750V offset...
  • Page 186 25471 20560 24794 20239 StdDev Bottom Probe 662V Sensitivity:45 Meas #1:300 Light 17888 5495 Dark 10645 1683 Delta 7243 3812 7389 3885 7167 3756 StdDev #2:485 Light 35657 22859 Dark 10645 1681 Delta 25012 21178 25211 21448 24799 20920 StdDev CALIBRATION Carrier - Top Mono Fluorescence Upper Left...
  • Page 187 Upper Right x= 8912 y= 6612 Delta 1 -856 - -852= Delta 2 8912 - 8916= Delta 3 6612 - 6612= Delta 4 404 - 400= Carrier - Top Filter Fluorescence Upper Left x=-3672 y= 6612 Lower Left x=-3668 Lower Right x= 6096 Upper Right x= 6096...
  • Page 188 0000 Dispenser 1: 005.2,010.5,020.5,040.4,080.4,200.4 Dispenser 2: 005.2,010.2,020.2,040.1,079.9,199.8 Filter Cube: Default Filter Set 1: Blue Ex: 360/40 Mirror: Top 400 nm Em: 460/40 Filter Set 2: Green Ex: 485/20 Mirror: Top 510 nm Em: 528/20 Reviewed/Approved By: _______________________________________ Date: ________________...
  • Page 189 Absorbance Test Plate Results Reader: Synergy H1 (Serial Number: 1506126) Basecode: P/N 8040200 (v2.00) Date and Time: 6/12/2015 1:03:23 PM Absorbance Plate: 7 Filter Test Plate (P/N 7260522) - S/N 253006 Last Plate Certification: October 2014 Next Plate Certification Due: October 2015...
  • Page 190 Repeatability Results Wells Read 1 0.134 0.443 0.869 1.271 1.238 1.586 Min Limit 0.128 0.433 0.855 1.253 1.221 1.565 Max Limit 0.140 0.452 0.882 1.289 1.256 1.607 Read 2 0.134 0.443 0.869 1.271 1.238 1.586 Result PASS PASS PASS PASS PASS PASS Reviewed/Approved By: _______________________________________...

Table of Contents

Save PDF