Agilent Technologies 1290 Infinity User Manual page 194

4
Appendices
DLW Principles and Operating Details
Table 37
Cycle Description
The Injection Valve is switched to position
Active.
The time Pre Inject Delay is awaited.
The loop is filled with the sample volume as
specified in the sample List.
The Injection Valve is switched to position
Standby, the loop content is injected.
Timer 1 (Delay Stator Wash is started and a
start signal to the HPLC system is sent.
The plunger of the DLW Syringe is pushed down
to dispense the Rear Sample and Air Segment
to Waste. The Holding Loop is still filled with
Wash Solvent 1.
The DLW Actuator/Solenoid is activated to
deliver Wash Solvent 2 into the Holding Loop to
clean the Injection Valve from Port 1 to Port 2.
For this step the needle tip is lifted, releasing the
sealing pressure to enable rinsing around the tip
sealing point.
The Injection Unit is moved to the DLW Wash
Station, position Wash2.
The needle is rinsed inside and out with Wash
Solvent 2.
The Injection Unit is moved back to the Injection
Valve. The Inlet Port and engraving to waste Port
is flushed with Wash Solvent 1 to prepare the
valve for the next injection.
The Injection Unit is moved back to the DLW
Wash Station, position Wash1 to flush the
syringe needle inside and out with Wash
Solvent 1.
This is a preparation step for next injection.
Especially important for biofluid samples.
Cycle end for LC-Inj DLW Standard macro.
194
LC-Inj DLW Standard Rev03
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Cycle Variable
Inject to
Pre Injection Delay
(SL.volume)
Injection Speed
Post Inject Delay
Timer 1
(Syr. Eject Speed)
Wash2
Inject to
Needle Gap Valve Clean
Valve Clean Time Solvent 2
N O T E
The Needle Gap Valve Clean is explained in
detail in the macro DLW Priming.
Wash2
(Syr.Eject Speed)
Post Clean Time Solvent 2
Wash1
Inject to
Needle Gap Valve Clean
Valve Clean Time Solvent 1
Wash1
Post Clean Time Solvent 1