Agilent Technologies 1290 Infinity User Manual page 190

4
Appendices
DLW Principles and Operating Details
General Considerations
The duration of the wash steps has to be established for each configuration
and application. The viscosity and surface tension of the individual wash
solvent composition and the backpressure of the system are factors to
consider.
Be aware that a higher backpressure builds-up if the valve bore size (standard
valve bore 0.25 mm) or the installed loop internal diameter are lower.
Standard loop internal diameter (ID) for Agilent Technologies defined loops
with a volume of 5 , 10 and 20 µL is 0.25 mm. The loop with 2 µL content
volume has an ID of 0.125 mm.
Keep the tubing internal diameters of the tubing in line with the valve
dimensions, loop ID and flow rate. For details see poster Tips & Hints for
HPLC Technique.
Priming the Solvent Lines, Wash1 and Wash2
This macro is used at installation to prime the entire system. Set the wash
time to approx. 120 seconds for each solvent. After installation, for daily
routine it is recommended to prime the system before the first run is
activated. For daily preparation of the system, the wash time can be much
shorter, approx. 20 seconds. The goal is an entire liquid system free of any air
bubbles.
190 Agilent 1290 Infinity LC Injectors HTC/HTS User Manual