Agilent Technologies 1290 Infinity II User Manual
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Agilent Technologies 1290 Infinity II User Manual

Preparative open-bed fraction collector
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Summary of Contents for Agilent Technologies 1290 Infinity II

  • Page 1 Agilent InfinityLab LC Series 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Agilent Technologies...
  • Page 2 (June 1987) or any equivalent agency regu- lation or contract clause. Use, duplication or disclosure of Software is subject to Agi- lent Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 3 7 Maintenance This chapter describes the maintenance of the module. 8 Parts for Maintenance and Repair This chapter provides information on parts for maintenance and repair. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 4 This chapter provides information on cables used with the module. 10 Hardware Information This chapter describes the module in more detail on hardware and electronics. 11 Appendix This chapter provides additional information on safety, legal, and web. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 5: Table Of Contents

    Replace Inlet/Waste Tubings 4 Preparing the Fraction Collector Best Practices Solvent Information Capillary Color Coding Guide Installing Capillaries Setting up the Fraction Collector with the Instrument Control Interface Method Parameter Settings Pooling 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 6 8 Parts for Maintenance and Repair Supported Containers List of Recommended Fraction Tubes Fraction Collector Accessory Kit Tubing Kits Top Fume Hood Kit 9 Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 7 Electrical Connections Interfaces Setting the 6-bit Configuration Switch Early Maintenance Feedback Instrument Layout 11 Appendix General Safety Information Waste Electrical and Electronic Equipment Directive Radio Interference Sound Emission Agilent Technologies on Internet 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 8 Contents 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 9: Introduction

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Introduction Product Description Features Overview of the Module Fraction Collector Principle Leak and Waste Concept This chapter gives an introduction to the module and an instrument overview. Agilent Technologies...

  • Page 10: Product Description

    Introduction Product Description Product Description The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector is the ideal solution that automatically manages high-capacity fraction collection of purified peaks for semi-preparative and preparative scale purification. Collect milligrams to multi-gram quantities of sample intuitive with ease. The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector features handling a wide variety of collection vessels with ease and capacity of up to 5.9 L by using...

  • Page 11: Features

    Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector can collect a maximum of 5.9 L. • Scalable collection volumes – The Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector enhances flexibility by providing two differently optimized tubing kits. Optimized for collections up to 50 mL/min and the other high flow kit for up to 200 mL/min.

  • Page 12: Overview Of The Module

    The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet. The rinse waste produced during this operation is channeled safely away through a waste drain. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 13: Fraction Collector Principle

    Before the start of the sequence, and during an analysis, the diverter valve is in the fraction start position. In this position, the mobile phase flows through the diverter valve towards waste. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 14: Leak And Waste Concept

    Introduction Leak and Waste Concept Leak and Waste Concept Rinse Port Drain Outlet 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 15: Site Requirements And Specifications

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 16: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 17 Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. Power cords WA R N I N G Solvents may damage electrical cables.
  • Page 18 ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 19: Physical Specifications

    For storing the module Safety standards: Installation category II, Pollution degree 2 For indoor use only. IEC, EN, CSA, UL ISM Classification ISM Group 1 Class B According to CISPR 11 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 20: Performance Specifications

    When a peak is discovered by a peak trigger, fractions are collected in dedicated vessels. Depending on the max. fill volume, Recovery and Fractions will use one or more vessel(s). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 21 G6130BA, LC/MS Single Quad SL G7121A, 1260 Infinity II FLD G4260B, 1260 Infinity II ELSD G7162A, 1260 Infinity II RID Diverter valve 3/2 valve Maximum pressure 6 bar (switching) Drawers Drawer ambient 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 22 Tube Container for 12 x 150 mm tubes, ambient, 72 tubes Tube Container for 12 x 100 mm tubes, ambient, 72 tubes Minium tube height 50 mm Maximum tube height 160 mm 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 23: Using The Fraction Collector

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Using the Fraction Collector Solvent Information Turn on/off Status Indicators Drawer Status Indicator Exchange Drawers Exchange Containers Replace Inlet/Waste Tubings This chapter explains the essential operational parameters of the module. Agilent Technologies...

  • Page 24: Solvent Information

    • Chromatographic grade ethers, which can contain peroxides (for example, THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 25 For example, a 1 % solution of acetic acid in methanol will attack steel. • Solutions containing strong complexing agents (for example, EDTA, ethylene diamine tetra-acetic acid). • Mixtures of carbon tetrachloride with 2-propanol or THF. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 26: Turn On/Off

    Using the Fraction Collector Turn on/off Turn on/off Plug in the power cable. Power switch 1: On 2: Off Unplug the power cable. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 27: Status Indicators

    5. Resident mode (blinking) - for example during update of main firmware. 6. Bootloader mode (fast blinking). Try to re-boot the module or try a cold-start. Then try a firmware update. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 28: Drawer Status Indicator

    2: on: drawer detected and not in use = drawer can be pulled out 3: blinking: drawer detected and in use = drawer blocked Do not remove the drawer when the drawer status indicator is blinking. N O T E 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 29: Exchange Drawers

    WA R N I N G Open drawers protrude from the module. Crashing into open drawers can lead to injuries and damage to the module. ➔ Always remove or close a drawer completely. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 30 Using the Fraction Collector Exchange Drawers Remove the drawer. Release the drawer by pushing the metal plate underneath the drawer with the second hand. Exchange the drawer Install the drawer. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 31: Exchange Containers

    Lift the container up at the circular handles. the drawer. Insert the new container. N O T E Mind the correct orientation of the container on the drawer. Check if the container is fixed properly ("Click"). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 32: Replace Inlet/Waste Tubings

    Swing up the fume hood against the mechanical stop. Remove the fume hood by pulling it toward you. Remove the Inlet/Waste Tubings from the capillary Remove the tubing clip from the fraction collector. holder. Unlock (Backside) Pull Tilt 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 33 Unscrew the tubings from the valve assembly. Screw the tubings of the new inlet/waste tubing kit into the valve assembly. Tighten the fittings finger-tight, then use the Fitting handle (about ¼ turn). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 34 Make sure that the holder is fixed properly. Press the small button to insert the tubing properly. Route the tubings clockwise (one turn) around the Install the tubings to the tube holder. robotics arm. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 35 Install the tubing clip to the fraction collector. Push the clip so that it rests against the housing. Tilt Push Lock (backside) Make sure the clip rests firmly against the housing. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 36 Install the Inlet/Waste Tubings to the capillary holder. Push the top fume hood into the recession. Position them approximately in the middle of the capillary holder. Close the top fume hood. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 37: Preparing The Fraction Collector

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Preparing the Fraction Collector Best Practices Regular Inspections Power up / Shut down Prepare the Fraction Collector Using the Fraction Collector Solvent Information Material Information Capillary Color Coding Guide Installing Capillaries...

  • Page 38: Best Practices

    Using the Fraction Collector • Rinse the needle between runs. • Pooling: Make sure that all fraction collection locations are large enough to completely collect all pooled fractions. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 39: Solvent Information

    Recommended Wash Solvents • water • ethanol • methanol • water/acid (especially for basic compounds) • water/base (especially for acidic compounds) • water/acetonitrile 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 40: Material Information

    (cresols, salicylic acid etc.). When used above room temperature, PEEK is sensitive to bases and various organic solvents, which can cause it to swell. Under such conditions normal 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 41 It can be corroded by strong bases (e.g. hydroxide solutions > 10 %, diethylamine). It is not recommended for the use with fluoric acid and fluorides. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 42 (about pH 0.1) at room temperature is about 13 μm/year. At room temperature, titanium is resistant to concentrations of about 5 % sulfuric acid (about pH 0.3). Addition of nitric acid to hydrochloric or sulfuric acids 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 43 Zirconium Oxide is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. Platinum/Iridium Platinum/Iridium is inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 44 Sapphire, Ruby and Al -based ceramics Sapphire, ruby and ceramics based on aluminum oxide Al are inert to almost all common acids, bases and solvents. There are no documented incompatibilities for HPLC applications. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 45: Capillary Color Coding Guide

    Preparing the Fraction Collector Capillary Color Coding Guide Capillary Color Coding Guide Figure 1 Syntax for capillary description 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 46: Installing Capillaries

    The capillaries mentioned above are examples only. Select a nut that is long enough for the fitting you'll be Slide the nut over the end of the tubing. using. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 47 N O T E Don't overtighten. Overtightening will shorten the lifetime of the fitting. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 48 If changing from a column or an injection valve to another, the fitting may leak or decrease the quality of the separation by contributing to band broadening. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 49: Setting Up The Fraction Collector With The Instrument Control Interface

    IP address option and enter the appropriate value for the host computer to enable automatic detection of the hardware configuration. The system configures the instrument automatically with no further manual configuration necessary. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 50 Preparing the Fraction Collector Setting up the Fraction Collector with the Instrument Control Interface Figure 3 Instrument Configuration 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 51 • Device name, • Type ID, • Button: Connection settings Module List: • Module identifier (Type ID: Serial number), • Device name, • Button: Configure (Device name, Serial number, Firmware revision) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 52 • If your system is configured with more than one Agilent pump, click the down-arrow and select the pump that delivers the main flow to the Infinity II Fraction Collector. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 53: Fraction Collector User Interface (Dashboard Panel)

    The fraction location currently in use. • Fraction mode: The current fraction mode. • Purge Status: The status of the purge procedure. • Flush Status: The status of the Flush procedure. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 54 The list includes all pumps that can be used as linked pump. Choose None if the pump that delivers the main flow does not support linking. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 55: Method Parameter Settings

    Preparing the Fraction Collector Method Parameter Settings Method Parameter Settings Figure 4 Method settings 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 56 You can Limit Peak Duration to stop the fraction collection in cases where the baseline drifts and the signal does not drop below the specified threshold value. Limits: 1 – 10000 s 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 57 Use the Fraction Preview screen to test the fraction collection parameters against one or more reference signals. You can also use the Fraction Preview to optimize the fraction collection parameters interactively. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 58: Advanced Settings

    The Fraction Collector method setup advanced parameters are in three sections, depending on the configuration: • Delay Settings • Fill Volume Settings • 3rd Party Pump Flow (only visible if there is no Agilent pump recognized.) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 59 If your Fraction Collector is not connected to a Linked Pump, specify a Pump Flow for the Fraction Collection method. N O T E This section is only visible if there is no Agilent pump recognized. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 60: Timetable Settings

    The values in the fraction collector timetable change instantaneously at the time defined in the timetable. The following parameters can be changed: • Fraction Mode • Trigger Settings 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 61 Off, where when the peak detector encounters a peak, the peak is collected independently of the volume slices, specified by the volume of the fractions to collect in the Volume slices field) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 62 You can select from: • No Timeout (The peak duration has no limit) • Use Max Peak Duration (The peak has a maximum duration, set in the Maximum Peak Duration field) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 63: Pooling

    If a location exceeds its maximum fill volume during pooling, the fraction collection is stopped with an error condition. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 64 Preparing the Fraction Collector Pooling 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 65: Troubleshooting And Diagnostics

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Troubleshooting and Diagnostics User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces. Agilent Technologies...

  • Page 66: User Interfaces

    67. • The Agilent OpenLab ChemStation C.01.03 and above do not include any maintenance/test functions. • Screenshots used within these procedures are based on the Agilent Lab Advisor Software. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 67: Agilent Lab Advisor Software

    The tests and diagnostic features that are provided by the Agilent Lab Advisor software may differ from the descriptions in this manual. For details, refer to the Agilent Lab Advisor software help files. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 68 Troubleshooting and Diagnostics Agilent Lab Advisor Software 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 69: Error Information

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Error Information What Are Error Messages General Error Messages Timeout Shutdown Remote Timeout Lost CAN Partner Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak...

  • Page 70: What Are Error Messages

    In all cases, error propagation is done via the CAN bus or via an APG/ERI remote cable (see documentation for the APG/ERI interface). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 71: General Error Messages

    Check the logbook for the occurrence and A not-ready condition was present during a source of a not-ready condition. Restart the sequence or multiple-injection run for a analysis where required. period longer than the timeout threshold. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 72: Shutdown

    Check the vacuum degasser for an error The degasser failed to generate sufficient condition. Refer to the Service Manual for the vacuum for solvent degassing. degasser or the pump that has the degasser built-in. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 73: Remote Timeout

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 74: Lost Can Partner

    Exchange the CAN cable. Defective CAN cable. Switch off the system. Restart the system, and Defective main board in another module. determine which module or modules are not recognized by the system. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 75: Leak Sensor Short

    Ensure the leak sensor is connected correctly. Leak sensor not connected to the main board. Exchange the leak sensor. Defective leak sensor. Exchange the leak sensor. Leak sensor incorrectly routed, being pinched by a metal component. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 76: Compensation Sensor Open

    If the resistance across the sensor falls below the lower limit, the error message is generated. Probable cause Suggested actions Exchange the main board. Defective main board. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 77: Fan Failed

    This changes the resistance of the leak sensor which is sensed by the leak-sensor circuit on the main board. Probable cause Suggested actions Ensure all fittings are tight. Loose fittings. Exchange defective capillaries. Broken capillary. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 78 Error Information General Error Messages 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 79: Maintenance

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Maintenance Introduction to Maintenance Warnings and Cautions Cleaning the Module Overview of Maintenance Install and Remove the Top Fume Hood Clean the Leak Pan Prepare the Module for Transportation Replace the Module Firmware This chapter describes the maintenance of the module.

  • Page 80: Introduction To Maintenance

    The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system stack. There are no serviceable parts inside. N O T E Do not open the module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 81: Warnings And Cautions

    WA R N I N G ➔ Regularly inspect the inlet / waste tubing assembly and the valve to needle tubing and exchange them if they are worn out or show visible signs of damage. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 82 If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 83: Cleaning The Module

    WA R N I N G hazard and damage the module ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any connections in the flow path. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 84: Overview Of Maintenance

    Overview of maintenance procedures Procedure Typical Frequency Notes Replacing the Inlet / Waste When worn out, when “Replace Inlet/Waste tubings showing visual signs of Tubings” on page 32 damage, typically once per year. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 85: Install And Remove The Top Fume Hood

    Install the exhaust sheet by fixing the four screws to the Push the top fume hood into the recession. fraction collector. Close the top fume hood. Swing up the fume hood against the mechanical stop. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 86 Install and Remove the Top Fume Hood Remove the fume hood by pulling it toward you. Remove the screws fixing the exhaust sheet to the fraction collector, then remove the exhaust sheet. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 87: Clean The Leak Pan

    If the release knobs do not snap in, slightly turn them. RFID antenna Guide support assembly Leak pan N O T E Don't touch or clean the RFID antennas on the back side (lower side) of the guide support assembly. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 88: Prepare The Module For Transportation

    • Remove all containers and drawers. • Remove all vessels from the rinse port. Insert the transportation foam to the rear panel. Push the transportation foam down to the rinse port. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 89 For shipping the module to another location, position the module in the transportation box and fix it correctly. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 90: Replace The Module Firmware

    Agilent web. http://www.agilent.com/en-us/firmwareDownload?whid=69761 2 For loading the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 91: Parts For Maintenance And Repair

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Parts for Maintenance and Repair Supported Containers List of Recommended Fraction Tubes Fraction Collector Accessory Kit Tubing Kits Top Fume Hood Kit This chapter provides information on parts for maintenance and repair.

  • Page 92: Supported Containers

    Tube Container for 16 mm x 100 mm tubes, ambient, 36 tubes G9321-60131 Tube Container for 12 mm x 150 mm tubes, ambient, 72 tubes G9321-60045 Tube Container for 12 mm x 100 mm tubes, ambient, 72 tubes 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 93: List Of Recommended Fraction Tubes

    Fraction Tubes 150 mm x 12 mm, 11 mL (pack of 250) fits with p/n G9321-60131 5022-6531 Fraction Tubes 100 mm x 12 mm, 7 mL (pack of 250) fits with p/n G9321-60045 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 94: Fraction Collector Accessory Kit

    Parts for Maintenance and Repair Fraction Collector Accessory Kit Fraction Collector Accessory Kit Description G9321-68002 Fraction Collector Accessory Kit 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 95: Tubing Kits

    Fitting 1/4-28 for Tube-OD 2.5 mm ESD-PEEK 5023-2872 Fitting 1/4-28 for Tube-OD 2.0 mm ESD-PEEK 5043-1471 Fitting Handle G9321-20052 Tubing Segment G9321-87712 Label Tubing IN ID1.2 G9321-87716 Label Tubing Out To Waste 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 96 Fitting 1/4-28 for Tube-OD 2.5 mm ESD-PEEK 5023-2874 Fitting 1/4-28 for Tube-OD 1.6 mm ESD-PEEK 5043-1471 Fitting Handle G9321-20052 Tubing Segment G9321-87708 Label Tubing IN ID0.8 G9321-87716 Label Tubing Out To Waste 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 97: Top Fume Hood Kit

    Top Fume Hood Kit contains the following items: G9321-60830 Top Fume Hood 0515-1753 SCREW-MACH-M3X0.5-8MM-LG-PAN-HD G9321-00100 Exhaust Sheet G1364-03201 Exhaust Tube Adapter 5067-5406 Screw Tx20, M4 x 8 mm Figure 7 Top Fume Hood Kit 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 98 Parts for Maintenance and Repair Top Fume Hood Kit 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 99: Identifying Cables

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Identifying Cables Cable Overview Analog Cables Remote Cables CAN/LAN Cables RS-232 Cables This chapter provides information on cables used with the module. Agilent Technologies...

  • Page 100: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
  • Page 101 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m USB cables Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 102: Analog Cables

    The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent Signal Name module Not connected Shield Analog - Center Analog + 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 103 Pin Agilent Signal Name module Shield Shield Analog - Center Center Analog + Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 104: Remote Cables

    NOT USED pink SHUT DOWN blue START PREPARE black 1wire DATA violet DGND grey-pink +5V ERI out red-blue PGND white-green PGND brown-green +24V ERI out white-yellow +24V ERI out yellow-brown 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 105 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 106 Ground Cable Shielding One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 107 Pin Agilent Signal Name Active module (TTL) White Digital ground Brown Prepare run Gray Start Blue Shut down Pink connected Yellow Power on High Ready High Green Stop Black Start request 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 108: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 109: Cables

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 110: Usb

    To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 111: Hardware Information

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Hardware Information Firmware Description Electrical Connections Serial Number Information (ALL) Rear View of the Module Interfaces Overview Interfaces ERI (Enhanced Remote Interface) USB (Universal Serial Bus) Setting the 6-bit Configuration Switch...

  • Page 112: Firmware Description

    • run synchronization through APG remote, • error handling, • diagnostic functions, • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 113 Update of main system can be done in the resident system only. Update of the resident N O T E system can be done in the main system only. Main and resident firmware must be from the same set. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 114 All these specific informations are described in the documentation provided with the firmware update tools. The firmware update tools, firmware and documentation are available from the Agilent web. • http://www.agilent.com/en-us/firmwareDownload?whid=69761 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 115: Electrical Connections

    There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.

  • Page 116: Serial Number Information (All)

    Alphabetic character A-Z (used by manufacturing) Alpha-numeric code 0-9, A-Z, where each combination unambiguously denotes a module (there can be more than one code for the same module) 00000 Serial number 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 117: Rear View Of The Module

    Hardware Information Electrical Connections Rear View of the Module 6-bit Configuration switch Power socket Figure 9 Rear view of the fraction collector 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 118: Interfaces

    G7121A/B FLD G7162A/B RID G7165A MWD Fraction Collectors G7159B FC G7166A VFC Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 119 • RS-232C as interface to a computer • USB (Universal Series Bus) as interface to a computer • REMOTE connector as interface to other Agilent products • Analog output connector(s) for signal output 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 120: Overview Interfaces

    “USB (Universal Serial Bus)” on page 125. Analog Signal Output The analog signal output can be distributed to a recording device. For details refer to the description of the module’s main board. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 121 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 122 PREPARE (L) Request to prepare for analysis (for example, calibration, detector lamp on). Receiver is any module performing pre-analysis activities. Special Interfaces There is no special interface for this module. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 123: Eri (Enhanced Remote Interface)

    This way the interface can support various additional devices like sensors, triggers (in and out) and small controllers, etc. Figure 10 Location of the ERI interface (example shows a G7114A/B VWD) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 124 This serial line can be used to read out an EPROM or write into an EPROM of a connected ERI-device. The firmware can detect the connected type of device automatically and update information in the device (if required). 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 125: Usb (Universal Serial Bus)

    • Short circuit will be detected through hardware. USB (Universal Serial Bus) USB (Universal Serial Bus) - replaces RS232, supports: • a PC with control software (for example Agilent Lab Advisor) • USB Flash Disk 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 126: Setting The 6-Bit Configuration Switch

    • For specific LAN modes switches 4-5 must be set as required. • For boot resident/cold start modes switches 1+2 or 6 must be UP. Figure 11 Location of Configuration switch (example shows a G7114A/B VWD) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 127 When selecting mode COM, settings are stored to non-volatile memory. When selecting mode TEST, COM settings are taken from non-volatile memory. not assigned - Always keep these switches on position ‘0’ (off) Default IP Address is 192.168.254.11 Host Name will be the MAC address. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 128: Special Settings

    ➔ Save your methods and data before executing a forced cold start. If you use the following switch settings and power the instrument up again, it will start as described above. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 129 Boot Main System / Keep Data Boot Resident System / Keep Data Boot Main System / Revert to Default Data Boot Resident System / Revert to Default Data Note: The setting '0' (down) is essential. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 130: Early Maintenance Feedback

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 131: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 132 Hardware Information Instrument Layout 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 133: Appendix

    1290 Infinity II Preparative Open-Bed Fraction Collector User Manual Appendix General Safety Information General Safety Information Safety Standards General Before Applying Power Ground the Instrument Do Not Operate in an Explosive Atmosphere Do Not Remove the Instrument Cover Do Not Modify the Instrument...

  • Page 134: General Safety Information

    Do not use this product in any manner not specified by the manufacturer. The protective features of this product may be impaired if it is used in a manner not specified in the operation instructions. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 135: Before Applying Power

    Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. ➔ Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations. ➔...

  • Page 136: Do Not Operate In An Explosive Atmosphere

    Personal injury (for example electrical shock, intoxication) ➔ Instruments that appear damaged or defective should be made inoperative and secured against unintended operation until they can be repaired by qualified service personnel. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 137: Solvents

    For details, see the usage guideline for the solvent cabinet. A printed copy of the guideline N O T E has been shipped with the solvent cabinet, electronic copies are available in the Agilent Information Center or via the Internet. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 138: Safety Symbols

    A pacemaker could switch into test mode and cause illness. A heart defibrillator may stop working. If you wear these devices keep at least 55 mm distance to magnets. Warn others who wear these devices from getting too close to magnets. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 139 ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 140: Waste Electrical And Electronic Equipment Directive

    Monitoring and Control Instrumentation product. Do not dispose of in domestic household waste N O T E To return unwanted products, contact your local Agilent office, or see http://www.agilent.com for more information. 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 141: Radio Interference

    Appendix Radio Interference Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or...

  • Page 142: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...

  • Page 143: Agilent Technologies On Internet

    Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 144 CAN partner guide resident system color update tool coding updates 113, maintenance guide upgrade/downgrade definition of compensation sensor open frequency range feedback compensation sensor short 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 145 (ERI) remote waste cables electrical and electronic repairs equipment replacing firmware WEEE directive RS-232C weight cable safety class I safety general information standards symbols 1290 Infinity II Preparative Open-Bed Fraction Collector User Manual...
  • Page 146 In This Book This manual contains technical reference information about the Agilent 1290 Infinity II Preparative Open-Bed Fraction Collector (G7159B). The manual describes the following: • introduction, • requirements and specifications, • using the fraction collector, • optimizing, • troubleshooting and diagnostics, •...

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