Agilent Technologies 1290 Infinity User Manual page 162

3
LC Injector HTC/HTS Description and Installation
Troubleshooting
Carry-over Test with UV-Detection
• • Test Sample: Chlorhexidine, 0.6 mg/mL dissolved in Water : Acetonitrile =
• Blank Solution: Water : Acetonitrile + 0.1 %TFA
• HPLC System:
• Wash Solvents:
• Loop Size:
• Injection Valve: VICI/Valco C72VX-1696D-CTC, bore size 0.25 mm
• Cycle (ICC-CE): DLW Standard
• Method Parameter Settings
162
90 : 10 + 0.1 % TFA
• Binary Solvent Delivery Pump
• UV-Detector, Wavelength: 257 nm
• HPLC Column: C18 (Halo), 2.1 x 50 mm; spherical 2.7 µm
• Column Temperature: Ambient
• Mobile Phase: A: Water + 0.1 % TFA; B: Acetonitrile + 0.1 % TFA
• Gradient:
• 10 % A hold for 0.5 min
• to 90 % B in 7.5 min
• hold for 1 min
• Wash1: Water + 0.1 % TFA
• Wash2: Acetonitrile + 0.1 % TFA
• 2 µL; Loop ID 0.13 mm
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual