Agilent Technologies 1290 Infinity User Manual page 149

Excessive carryover between samples
Probable cause
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Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
Dirty syringe and/or valve injection port.
Inappropriate wash solvent(s).
Waste tubing ID at injection valve is too
small, causing waste liquid to be pulled
back, by capillary action, into rotor groove.
Damaged or grooved valve rotor.
Valve needle seal leaks.
Inappropriate wash solvent composition.
Tube of Holding Loop at end not cut square
or nut/ferrule fixation not correct.
DLW Actuator/Solenoid questionable,
defect.
Solvent Frits in wash solvent reservoir
blocked.
LC Injector HTC/HTS Description and Installation
Suggested actions
Increase Pst Inj Slv1/Slv2 values in method.
Use PreClnSlv1/Slv2 and PreClnSpl.
Use appropriate wash solvent(s).
Replace the waste tubing with larger ID tubing.
Replace valve rotor, see
page 108.
Change valve needle seal, see
Guide" on page 112).
Use appropriate wash solvent. Observe also the
order of use for biological samples. First wash is
always an aqueous solution followed by organic
solvents.
Check the connections from the Holding Loop
(Is the pilot distance appropriate? Is the tube
end damaged? Is the tube end cut absolutely
square? Are the right nuts and ferrules used?).
Verify the functionality of the DLW Actuator. Is
solvent flowing when active? Use command
Utilities/Wash Station for tests.
• Clean the frits in a ultrasonic bath with an
appropriate solvent.
• If flow is not ensured after cleaning, replace
frits.
• Replace wash solvent and clean reservoir
bottle and tubing carefully.
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Troubleshooting
"Installation" on
"Valve Needle
149