Agilent Technologies 1290 Infinity User Manual page 164

3
LC Injector HTC/HTS Description and Installation
Troubleshooting
Repeatability Test with UV-Detection
• Test Sample
• HPLC System
• Wash Solvents:
• Loop Size: 2 µL (Loop overfill, 7 µL); Loop ID: 0.13 mm; 20 µL (partial Loop,
• Injection Valve: VICI/Valco C72VX-1696D-CTC, bore size 0.25 mm
• Method Parameter Settings
164
• Loop Overfill Caffeine, 520 mg/L
• Partial Loop Caffeine, 52 mg/L dissolved in Water : Acetonitrile = 90 :10
+ 0.1 % TFA
• Binary Solvent Delivery Pump
• UV-Detector, Wavelength: 273 nm
• HPLC Column: Zorbax SB-C18, 2.1 x 50 mm; spherical 1.8 µm
• Column Temperature: Ambient
• Mobile Phase: Water : Acetonitrile = 90 :10 + 0.1 % TFA
• Gradient: isocratic for 2 min
• Wash1: Water + 0.1 % TFA
• Wash2: Acetonitrile + 0.1 % TFA
10 µL); Loop ID 0.25 mm
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual