Agilent Technologies 1290 Infinity User Manual page 148

3
LC Injector HTC/HTS Description and Installation
Troubleshooting
Syringe does not fill properly.
Probable cause
1
Sample peaks/responses are not reproducible.
Probable cause
1
2
3
4
148
Air bubbles below Syringe plunger. Air
bubbles may remain below the plunger after
the first pull up. If the plunger is moved up
and down several times, these air bubbles
are worked out.
Dirty syringe.
Syringe pressure differences.
Vacuum created in sample vial.
Method Parameters
Suggested actions
• Increase method parameter "Fill Strokes".
• Check syringe parameter Eject Speed.
Suggested actions
• Increase Pst Cln Slv1/Slv2 values in method.
• Use PreClnSlv1/Slv2 and PreClnSpl.
Increase Pullup Delay value.
Reduce sample volume in sample vial. Use
setting under F3-Setup > System >
PrePressureVial.
Check recommended Method Parameters in
"Software Flow Chart"
following parameters are crucial and should be
verified first: Fill speed, Pullup delay, Injection
Speed, Post Inj Delay
Agilent 1290 Infinity LC Injectors HTC/HTS User Manual
on page 232. The