Agilent Technologies 1290 Infinity User Manual
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Agilent Technologies 1290 Infinity User Manual

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  • Page 1 Agilent 1290 Infinity Flexible Cube User Manual Agilent Technologies...
  • Page 2 1987) or any equivalent agency regulation or contract clause. Use, duplication or dis- closure of Software is subject to Agilent For Research Use Only Technologies’ standard commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will 1290 Infinity Flexible Cube User Manual...
  • Page 3 In This Guide... In This Guide... This manual covers the Agilent 1290 Infinity Flexible Cube (G4227A) 1 Introduction This chapter gives an introduction to the module, instrument overview and internal connectors 2 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications.
  • Page 4 This chapter provides information on parts and material required for the module. 11 Identifying Cables This chapter provides information on cables used with the 1290 series of HPLC modules. 12 Appendix This chapter provides addition information on safety, legal and web. 1290 Infinity Flexible Cube User Manual...

  • Page 5: Table Of Contents

    Setting up the Flexible Cube with Agilent ChemStation 6 Optimizing the Autosampler with the Flexible Cube Preparing the Autosampler and Flexible Cube How to Achieve Lowest Carry Over How to Achieve Highest Throughput Using the Flexible Cube 1290 Infinity Flexible Cube User Manual...
  • Page 6 Replacing Module Firmware 10 Parts and Materials for Maintenance Capillaries Parts overview Accessory Kits 11 Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC 1290 Infinity Flexible Cube User Manual...
  • Page 7 Contents 12 Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Flow Cell Agilent Technologies on Internet 1290 Infinity Flexible Cube User Manual...
  • Page 8 Contents 1290 Infinity Flexible Cube User Manual...

  • Page 9: Introduction

    1290 Infinity Flexible Cube User Manual Introduction Features Overview of the Module Flexible Cube Principle Early Maintenance Feetback Instrument Layout Electrical Connections Serial Number Information (ALL) Rear View of the Module Interfaces Overview Interfaces This chapter gives an introduction to the module, instrument overview and...

  • Page 10: Features

    Autosampler. For specifications, see “Performance Specifications” on page 34. This 1290 Infinity Flexible Cube has been introduced as part of the Agilent 1290 Infinity N O T E Liquid Chromatograph. 1290 Infinity Flexible Cube User Manual...

  • Page 11: Overview Of The Module

    It also includes a second valve drive slot. The initial revision of the Agilent 1290 Infinity Flexible Cube supports only the needle seat back flush option. Other options will follow later and will include a possibility to convert the G4226A Autosampler for fixed loop injections.

  • Page 12: Flexible Cube Principle

    Flexible Cube Principle Flexible Cube Principle The Flexible Cube is added to the 1290 Infinity system to reduce carry over in the Autosampler. This is achieved by flushing the needle seat and rotor seal with up to 3 different solvents which may have different properties and solvent strengths.
  • Page 13 1 Mainpass Figure 1 Mainpass The analytical pump is connected to the autosampler sampling loop, and the mobile phase is flowing through the autosampler injection loop continuously and thereby cleaning the insides of the tubings. 1290 Infinity Flexible Cube User Manual...
  • Page 14 2 Switch to Bypass Figure 2 Switch to Bypass The injection valve is switched to bypass, removing the injection loop from the high pressure flow path and connecting the flush pump with the needle seat. 1290 Infinity Flexible Cube User Manual...
  • Page 15 The first cleaning solvent available for flushing is normally of a high organic concentration with appropriate modifiers. This solvent should be able to dissolve most components of the sample, and thereby remove them from the Autosampler flow path. 1290 Infinity Flexible Cube User Manual...
  • Page 16 4 Flush with dissolving solvent #2 (B2) If this is not sufficient to reduce the carry over a dissolving solvent #2 may be used with either different modifiers and/or a really strong cleaning solvent. 1290 Infinity Flexible Cube User Manual...
  • Page 17 Any flushing solvent used must be miscible with previous solvent. If this is not the case it N O T E can result in precipitations, blockages or reduced performance of the instrument. 1290 Infinity Flexible Cube User Manual...
  • Page 18 6 Switch back to Mainpass Figure 5 Switch back to Mainpass When the complete flushing procedure has finished the autosampler switches back to Mainpass operation and thereby introduce the sample to the flow path. 1290 Infinity Flexible Cube User Manual...

  • Page 19: Early Maintenance Feetback

    EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. 1290 Infinity Flexible Cube User Manual...

  • Page 20: Instrument Layout

    • the plastic layers help cushion the electronic and mechanical parts from physical shock, and • the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself. 1290 Infinity Flexible Cube User Manual...

  • Page 21: Electrical Connections

    The security lever at the power input socket prevents the module cover from being taken off when line power is still connected. Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations.
  • Page 22 820 could be week 20 of 1998 or 2008 SSSSS real serial number Rear View of the Module Figure 6 Rear View of Flexible Cube – Electrical Connections and Label 1290 Infinity Flexible Cube User Manual...

  • Page 23: Interfaces

    The detector with on-board LAN is the preferred access point for control via LAN of the N O T E detector and/or the 1290 Infinity system. The inter-module communication is done via CAN. • CAN connectors as interface to other modules, •...
  • Page 24 There is no configuration possible on main boards with on-board LAN. These are N O T E pre-configured for • 19200 baud, • 8 data bit with no parity and • one start bit and one stop bit are always used (not selectable). 1290 Infinity Flexible Cube User Manual...
  • Page 25 Interfaces The RS-232C is designed as DCE (data communication equipment) with a 9-pin male SUB-D type connector. The pins are defined as: Table 2 RS-232C Connection Table Direction Function Ground Figure 7 RS-232 Cable 1290 Infinity Flexible Cube User Manual...
  • Page 26 APG Remote The APG Remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. Remote control allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
  • Page 27 START REQUEST (L) Request to start injection cycle (for example, by start key on any module). Receiver is the autosampler. Special Interfaces Some modules have module specific interfaces/connectors. They are described in the module documentation. 1290 Infinity Flexible Cube User Manual...
  • Page 28 Introduction Interfaces 1290 Infinity Flexible Cube User Manual...

  • Page 29: Site Requirements And Specifications

    1290 Infinity Flexible Cube User Manual Site Requirements and Specifications Site Requirements Physical Specifications Performance Specifications This chapter provides information on environmental requirements, physical and performance specifications. Agilent Technologies...

  • Page 30: Site Requirements

    ➔ Make sure the power connector of the instrument can be easily reached and unplugged. ➔ Provide sufficient space behind the power socket of the instrument to unplug the cable. 1290 Infinity Flexible Cube User Manual...
  • Page 31 Never operate your instrumentation from a power outlet that has no ground connection. ➔ Never use a power cord other than the Agilent Technologies power cord designed for your region. Use of unsupplied cables WA R N I N G Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury.
  • Page 32 Do not store, ship or use your module under conditions where temperature fluctuations could cause condensation within the module. ➔ If your module was shipped in cold weather, leave it in its box and allow it to warm slowly to room temperature to avoid condensation. 1290 Infinity Flexible Cube User Manual...

  • Page 33: Physical Specifications

    Up to 2000 m (6562 ft) Non-operating altitude Up to 4600 m (15091 ft) For storing the module Safety standards: Installation Category II, Pollution Degree 2 For indoor use IEC, CSA, UL only. Research Use Only. 1290 Infinity Flexible Cube User Manual...

  • Page 34: Performance Specifications

    Flushing is done in parallel to running analysis. Needle seat flush time to flush with 1 mL of solvent at maximum flow including solvent exchange typically requires 38 s. 1290 Infinity Flexible Cube User Manual...
  • Page 35 MS-detection: Sample : 50 ng/µl chlorhexidine (dissolved in mobile phase A), 1 µL injected and measured on → → Agilent 6460 QQQ (in specified conditions); MRM 1: 505.5 170 (CE: 36 V); MRM 3: 505.5 201.2 (CE: 20 V); fragmentor: 150 V, delta EMV(+): 200 V 1290 Infinity Flexible Cube User Manual...
  • Page 36 Site Requirements and Specifications Performance Specifications 1290 Infinity Flexible Cube User Manual...

  • Page 37: Installing The Module

    1290 Infinity Flexible Cube User Manual Installing the Module Unpacking the Module Optimizing the Stack Configuration One Stack Configuration Supported Two Stack Configurations Installing the Module Flow Connections to the Module This chapter gives information about the preferred stack setup for your system and the installation of your Flexible Cube.

  • Page 38: Unpacking The Module

    Unpacking the Module Damaged Packaging If the delivery packaging shows signs of external damage, please call your Agilent Technologies sales and service office immediately. Inform your service representative that the instrument may have been damaged during shipment. "Defective on arrival" problems C A U T I O N If there are signs of damage, please do not attempt to install the module.
  • Page 39 Flexible Cube Checklist Description Quantity Flexible Cube Power Cable User Manual Standard Accessory Kit (see “HPLC System Tool Kit” on page 112) Flexible Cube Accessory Kit (see “Flexible Cube Accessory Kit” on page 112) 1290 Infinity Flexible Cube User Manual...

  • Page 40: Optimizing The Stack Configuration

    Installing the Module Optimizing the Stack Configuration Optimizing the Stack Configuration If your module is part of a complete Agilent 1290 Infinity Liquid Chromatograph, you can ensure optimum performance by installing the following configurations. These configurations optimize the system flow path, ensuring minimum delay volume.

  • Page 41: One Stack Configuration

    Installing the Module Optimizing the Stack Configuration One Stack Configuration Figure 8 Supported One Stack Configuration (Front View) 1290 Infinity Flexible Cube User Manual...
  • Page 42 Installing the Module Optimizing the Stack Configuration Figure 9 Supported One Stack Configuration (Rear View) 1290 Infinity Flexible Cube User Manual...

  • Page 43: Supported Two Stack Configurations

    Installing the Module Optimizing the Stack Configuration Supported Two Stack Configurations Two Stack Configuration (Standard) Figure 10 Supported Two Stack Configuration Front View (Standard) 1290 Infinity Flexible Cube User Manual...
  • Page 44 Installing the Module Optimizing the Stack Configuration Figure 11 Supported Two Stack Configuration Rear View (Standard) 1290 Infinity Flexible Cube User Manual...
  • Page 45 Installing the Module Optimizing the Stack Configuration Two Stack Configuration (Thermostatted) Figure 12 Supported Two Stack Configuration Front View (Thermostatted) 1290 Infinity Flexible Cube User Manual...
  • Page 46 Installing the Module Optimizing the Stack Configuration Figure 13 Supported Two Stack Configuration Rear View (Thermostatted) 1290 Infinity Flexible Cube User Manual...

  • Page 47: Installing The Module

    Do not connect the power cable to the Instrument while the covers are removed. 1 Place the Flexible Cube in the stack, see “Optimizing the Stack Configuration” on page 40. 2 Ensure the line power switch on the front of the module is OFF (switch stands out). 1290 Infinity Flexible Cube User Manual...
  • Page 48 The module is turned on when the line power switch is pressed and the green indicator N O T E lamp is illuminated. The module is turned off when the line power switch is protruding and the green light is off. 1290 Infinity Flexible Cube User Manual...

  • Page 49: Flow Connections To The Module

    Do not leave the Autosampler to Flexible Cube capillary installed if the Flexible Cube is not part of the Software configuration or if turned off during operation. This procedure shows the Flexible Cube outside of a system. In an Agilent 1290 Infinity N O T E...
  • Page 50 Install the bottle head assemblies into the solvent Install low restriction needle seat from accessory kit into selection valve of the Flexible Cube. autosampler (see autosampler manual - Maintenance section for procedure). 1290 Infinity Flexible Cube User Manual...
  • Page 51 1290 Infinity Flexible Cube User Manual LAN Configuration LAN-Configuration This chapter provides information on connecting the module to the Agilent Data System. Agilent Technologies...

  • Page 52: Lan Configuration Lan-Configuration

    LAN-Configuration LAN-Configuration It is not recommended to connect an Agilent 1290 Infinity system via the G4227A Flexible Cube. The G4212A Diode Array Detector is producing the most data in the stack, followed by the G4220A Binary pump, and it is therefore highly recommended to use either of these modules for the LAN connection.

  • Page 53: Using The Module

    1290 Infinity Flexible Cube User Manual Using the Module Setting up the Flexible Cube with Agilent ChemStation The Flexible Cube User Interface Control Settings Method Parameter Settings Module Configuration This chapter provides information on how to set up the Flexible Cube for an analysis and explains the basic settings.

  • Page 54: Setting Up The Flexible Cube With Agilent Chemstation

    This section describes the Flexible Cube settings only. For information on the Agilent N O T E ChemStation or other 1290 Infinity modules refer to the corresponding documentation or the 1290 Infinity System Manual. After successful load of the ChemStation, you should see the module as an active item in the graphical user interface for the Autosampler (GUI).

  • Page 55: The Flexible Cube User Interface

    • Show the Control User Interface (special module settings) • Show the Method User interface (same as via menu Instrument – Setup G4226A • And Flexible Cube specific items: • Autoclean / Purge • Prime 1290 Infinity Flexible Cube User Manual...
  • Page 56 This feature primes the flush solvent inlet lines with fresh solvent. It can be configured what solvent lines to prime and additionally the needle wash port can also be primed with solvent. 1290 Infinity Flexible Cube User Manual...

  • Page 57: Control Settings

    These settings are available via right click on the Active Area of the ALS GUI. Linked Flexible Cube: To configure which Flexible Cube delivers flow to the Autosampler. Prime Flush Pump: Priming the Needle wash port for a given period of time. 1290 Infinity Flexible Cube User Manual...

  • Page 58: Method Parameter Settings

    Using the Module Setting up the Flexible Cube with Agilent ChemStation Method Parameter Settings These settings are available via Menu > Instrument > Setup Agilent 1290 Infinity Autosampler or via right click on the Active area. Figure 16 Method Parameter Settings...

  • Page 59: Module Configuration

    Do not leave the Autosampler to Flexible Cube capillary installed if the Flexible Cube is not part of the Software configuration or if turned off during operation. These settings are available via menu Instrument > More 1290 Infinity ALS > Control Configuration.
  • Page 60 Setting up the Flexible Cube with Agilent ChemStation Options: Lists installed options for the G4226A Autosampler. Flexible Cube installed: If the Flexible Cube option is checked the Flexible Cube cleaning features becomes activated in the Method and Configuration screens. 1290 Infinity Flexible Cube User Manual...

  • Page 61: Optimizing The Autosampler With The Flexible Cube

    1290 Infinity Flexible Cube User Manual Optimizing the Autosampler with the Flexible Cube Preparing the Autosampler and Flexible Cube How to Achieve Lowest Carry Over Internal Needle Wash External Needle Wash Needle Seat Back Flush Valve Rinse How to Achieve Highest Throughput Using the Flexible Cube This chapter provides information on how to optimize the Autosampler and the Flexible Cube for a minimum carry over and fastest cycle times.

  • Page 62: Preparing The Autosampler And Flexible Cube

    • Use inlet filters for flush solvent bottles. • It is recommended to sonicate solvent #3 which should be set to the chromatographic starting condition before use, to avoid generation of air bubbles. 1290 Infinity Flexible Cube User Manual...

  • Page 63: How To Achieve Lowest Carry Over

    Reducing the Carry over is an important part during any method development process. The flush solvents and flush timing has to be adjusted to the sample solvent, sample components injected and the mobile phase in use. 1290 Infinity Flexible Cube User Manual...
  • Page 64 Column related carry-over (all fittings and connections of the column, packing, stationary phase-sample interaction). The Agilent 1290 Infinity Autosampler is optimized for lowest carryover by careful design of the flow path and use of materials in which sample adsorption is minimized. A carryover amount of 0.002 % should be achievable even when a triple quadrupole mass spectrometer is used as detector.

  • Page 65: Internal Needle Wash

    Automated delay volume reduction (ADVR) and overlapped injection will reduce the delay volume but will also reduce the internal flushing of the Autosampler needle and should not be used with analytes where sample discrimination might be a problem. 1290 Infinity Flexible Cube User Manual...

  • Page 66: External Needle Wash

    The flush port and its peristaltic pump and tubing should be regularly flushed to ensure the lowest carryover. For example, before using the system each day, prime the flush port for three minutes with appropriate solvent. 1290 Infinity Flexible Cube User Manual...

  • Page 67: Needle Seat Back Flush

    This is one of the tasks that can be automated using the Flexible Cube module. Figure 18 Schematic of Autosampler with Flexible Cube Setup 1290 Infinity Flexible Cube User Manual...
  • Page 68 Any flushing solvent used must be miscible with previous solvent. If this is not the case it N O T E can result in precipitations, blockages or reduced performance of the instrument. 1290 Infinity Flexible Cube User Manual...

  • Page 69: Valve Rinse

    This ensures that the bypass groove in the rotor seal of the valve contains the gradient start conditions, which is especially important when using short narrow bore columns. 1290 Infinity Flexible Cube User Manual...
  • Page 70 N O T E additional switches must be taken into account when calculating the number of runs that can be executed until the rotor seal of the injection valve needs to be replaced (EMF). 1290 Infinity Flexible Cube User Manual...
  • Page 71 How to Achieve Lowest Carry Over Calculation of Time 1 Time 1 = Sample flush-out factor * Total flush-out volume/Flow rate Total flush-out volume = Injection volume + Seat capillary volume + Valve volume 1290 Infinity Flexible Cube User Manual...

  • Page 72: How To Achieve Highest Throughput Using The Flexible Cube

    Typically a flushing time of the needle seat of about 15 s at 4 mL/min for each solvent is sufficient or at least a good starting condition. 1290 Infinity Flexible Cube User Manual...

  • Page 73: Troubleshooting And Diagnostics

    1290 Infinity Flexible Cube User Manual Troubleshooting and Diagnostics Overview of the Module’s Indicators and Test Functions Status Indicators Power Supply Indicator Module Status Indicator User Interfaces Agilent Lab Advisor Software This chapter gives an overview about the troubleshooting and diagnostic features and the different user interfaces.

  • Page 74: Overview Of The Module's Indicators And Test Functions

    (see chapter Error Information). Diagnostic Signals The module has several signals (internal temperatures, voltages and currents of lamps) that can be used for diagnosing baseline problems; see Diagnostic Signals. 1290 Infinity Flexible Cube User Manual...

  • Page 75: Status Indicators

    Figure 20 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch. When the indicator is illuminated (green) the power is ON. 1290 Infinity Flexible Cube User Manual...

  • Page 76: Module Status Indicator

    • A fast red-blinking indicator indicates that the module is in boot loader mode (e.g. during update of main firmware). In such as case try to re-boot the module or try a cold-start. 1290 Infinity Flexible Cube User Manual...

  • Page 77: User Interfaces

    “Agilent Lab Advisor Software” on page 78. • The Agilent ChemStation B.04.02 and above do not include any maintenance/test functions. • Screenshots used within these procedures are based on the Agilent Lab Advisor Software. 1290 Infinity Flexible Cube User Manual...

  • Page 78: Agilent Lab Advisor Software

    Agilent Lab Advisor software may differ from the descriptions in this manual. For details refer to the Agilent Lab Advisor software help files. This manual provides lists with the names of Error Messages, Not Ready messages, and other common issues. 1290 Infinity Flexible Cube User Manual...

  • Page 79: Error Information

    1290 Infinity Flexible Cube User Manual Error Information What Are Error Messages General Error Messages Timeout Shut-Down Remote Timeout Synchronization Lost Leak Sensor Short Leak Sensor Open Compensation Sensor Open Compensation Sensor Short Fan Failed Leak Open Cover Cover Violation...

  • Page 80: What Are Error Messages

    (for example, repair, or exchange of consumables is necessary). In the event of such a failure, the red status indicator at the front of the module is switched on, and an entry is written into the module logbook. 1290 Infinity Flexible Cube User Manual...

  • Page 81: General Error Messages

    Check the logbook for the occurrence and A not-ready condition was present during a source of a not-ready condition. Restart the sequence or multiple-injection run for a analysis where required. period longer than the timeout threshold. 1290 Infinity Flexible Cube User Manual...

  • Page 82: Shut-Down

    Exchange the remote cable. Defective remote cable. Check the instrument for defects (refer to the Defective components in the instrument instrument’s documentation). showing the not-ready condition. 1290 Infinity Flexible Cube User Manual...

  • Page 83: Synchronization Lost

    Probable cause Suggested actions Please contact your Agilent service Defective flow sensor. representative. Please contact your Agilent service Leak sensor incorrectly routed, being representative. pinched by a metal component. 1290 Infinity Flexible Cube User Manual...

  • Page 84: Leak Sensor Open

    If the resistance across the sensor increases above the upper limit, the error message is generated. Probable cause Suggested actions Please contact your Agilent service Defective main board. representative. 1290 Infinity Flexible Cube User Manual...

  • Page 85: Compensation Sensor Short

    Probable cause Suggested actions Please contact your Agilent service Fan cable disconnected. representative. Please contact your Agilent service Defective fan. representative. Please contact your Agilent service Defective main board. representative. 1290 Infinity Flexible Cube User Manual...

  • Page 86: Leak

    Broken capillary. Open Cover The top foam has been removed. Probable cause Suggested actions Please contact your Agilent service Foam not activating the sensor. representative. Please contact your Agilent service Dirty or defective sensor. representative. 1290 Infinity Flexible Cube User Manual...

  • Page 87: Cover Violation

    Probable cause Suggested actions The top foam was removed during operation. Please contact your Agilent service representative. Please contact your Agilent service Foam not activating the sensor. representative. 1290 Infinity Flexible Cube User Manual...

  • Page 88: Module Spezific Error Messages

    This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the main board. Probable cause Suggested actions Ensure all fittings are tight Loose fitting Exchange defective tubing Broken tubing 1290 Infinity Flexible Cube User Manual...

  • Page 89: Flexcube Fan Failed

    Blocked needle seat Configure tubing to position 4 of injection valve. Tubing not correctly installed Configure Flexible Cube correctly Flexible Cube not configured in software Please contact your Agilent service Defective flush pump representative 1290 Infinity Flexible Cube User Manual...

  • Page 90: Valve Failed To Switch Or Didn't Initialize

    The valve drive has lost its position information and is not able to initialize. Probable cause Suggested actions • Check installation of valve head Valve drive mechanically blocked or defect • Please contact your Agilent service representative 1290 Infinity Flexible Cube User Manual...

  • Page 91: Maintenance And Repair

    1290 Infinity Flexible Cube User Manual Maintenance and Repair Introduction to Maintenance Warnings and Cautions Overview of Maintenance Cleaning the Module Exchange Flush Pump Inlet Valve Exchange Flush Pump Outlet Valve Exchange Valve Rotor Seal Replacing Module Firmware This chapter describes the maintenance of the module.

  • Page 92: Introduction To Maintenance

    Figure 21 on page 92 shows the main user accessible assemblies of the Agilent 1290 Infinity Flexible Cube. These parts can be accessed from the front (simple repairs) and don’t require to remove the Flexible Cube from the system stack.

  • Page 93: Warnings And Cautions

    ➔ Always unplug the power cable before opening the cover. ➔ Do not connect the power cable to the instrument while the covers are removed. 1290 Infinity Flexible Cube User Manual...
  • Page 94 C A U T I O N ➔ If you connect external equipment to the instrument, make sure that you only use accessory units tested and approved according to the safety standards appropriate for the type of external equipment. 1290 Infinity Flexible Cube User Manual...

  • Page 95: Overview Of Maintenance

    Maintenance and Repair Overview of Maintenance Overview of Maintenance The following pages describe maintenance procedures (simple repairs) that can be done without opening the main cover. 1290 Infinity Flexible Cube User Manual...

  • Page 96: Cleaning The Module

    WA R N I N G Liquid in the module electronics can cause shock hazard and damage the module. ➔ Do not use an excessively damp cloth during cleaning. ➔ Drain all solvent lines before opening any fittings. 1290 Infinity Flexible Cube User Manual...

  • Page 97: Exchange Flush Pump Inlet Valve

    In order to avoid leaks, place the solvent bottles at or below the level of the pump head. Open the front door of the module. Disconnect the finger tight fitting of the Inlet capillary at the bottom of the Flush pump. 1290 Infinity Flexible Cube User Manual...
  • Page 98 With a 14 mm wrench on the flange closest to the pump Remove the valve by moving it downwards. head, unscrew the valve. Insert the new valve. Tighten the valve with a 14 mm wrench attached to the flange closest to the pump head. 1290 Infinity Flexible Cube User Manual...
  • Page 99 Maintenance and Repair Exchange Flush Pump Inlet Valve Reconnect the finger tight fitting at the inlet of the flush Close the front cover and prime the flush pump before use pump. to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...

  • Page 100: Exchange Flush Pump Outlet Valve

    • Wrench open 14 mm (p/n 8710-1924) Parts required Description 5067-4716 Outlet valve Open the front door of the module. With a ¼ inch wrench disconnect the capillary at the top of the Outlet valve. 1290 Infinity Flexible Cube User Manual...
  • Page 101 With a 14 mm wrench on the flange closest to the pump Remove the valve by lifting it upwards. head, unscrew the valve. Insert the new valve. Tighten the valve with a 14 mm wrench attached to the flange closest to the pump head. 1290 Infinity Flexible Cube User Manual...
  • Page 102 Exchange Flush Pump Outlet Valve Reconnect the capillary with a ¼ inch wrench at the Close the front cover and prime the flush pump before use outlet of the flush pump. to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...

  • Page 103: Exchange Valve Rotor Seal

    Preparations In order to avoid leaks, place the solvent bottles at or below the level of the pump head. Open the front door of the module. Remove all capillaries from the valve head. 1290 Infinity Flexible Cube User Manual...
  • Page 104 Maintenance and Repair Exchange Valve Rotor Seal With a 9/64 inch hex key unscrew the stator screws. Remove the stator head and the stator ring. Remove the rotor seal. Remove the Isolation seal. 1290 Infinity Flexible Cube User Manual...
  • Page 105 Maintenance and Repair Exchange Valve Rotor Seal Replace the Isolation seal. Install new rotor seal. Install the stator head and the stator ring. With a 9/64 inch hex key alternately tighten the stator screws. 1290 Infinity Flexible Cube User Manual...
  • Page 106 Exchange Valve Rotor Seal Reinstall all capillaries to the valve head depending on the Close the front cover and prime the flush pump before use type and usage of the valve. to warrant trouble free usage. 1290 Infinity Flexible Cube User Manual...

  • Page 107: Replacing Module Firmware

    Update Tool and the documentation from the Agilent web. • http://www.chem.agilent.com/scripts/cag_firmware.asp. 2 To load the firmware into the module follow the instructions in the documentation. Module Specific Information There is no specific information for this module. 1290 Infinity Flexible Cube User Manual...
  • Page 108 Maintenance and Repair Replacing Module Firmware 1290 Infinity Flexible Cube User Manual...

  • Page 109: Parts And Materials For Maintenance

    1290 Infinity Flexible Cube User Manual Parts and Materials for Maintenance Capillaries Parts overview Accessory Kits This chapter provides information on parts and material required for the module. Agilent Technologies...

  • Page 110: Capillaries

    Parts and Materials for Maintenance Capillaries Capillaries Item p/n Description 5067-4680 Tubing Kit 600 mm; 130 bar G4280-67304 Solvent selection valve to flush pump tubing 5067-4697 Solvent selection valve bridge tubing 1290 Infinity Flexible Cube User Manual...

  • Page 111: Parts Overview

    Item p/n Description 5067-4717 Inlet valve 5067-4716 Outlet valve G4280-67304 Solvent selection valve to flush pump tubing 5067-4680 Tubing Kit 600 mm; 130 bar 5067-4697 Solvent selection valve bridge tubing Figure 22 Parts overview 1290 Infinity Flexible Cube User Manual...

  • Page 112: Accessory Kits

    5067-4680 Tubing Kit 600 mm; 130 bar 5067-4701 SS-Capillary_300x0.25mm ps ps 2sh 5181-1519 CAN cable, Agilent module to module, 1 m 5959-3878 CTN.SOLV.CABIN. 9301-1420 Solvent bottle, transparent G4220-60007 Bottle Head Assembly G4226-87012 Needle seat 1290 Infinity Flexible Cube User Manual...

  • Page 113: Identifying Cables

    1290 Infinity Flexible Cube User Manual Identifying Cables Cable Overview Analog Cables Remote Cables BCD Cables CAN/LAN Cables External Contact Cable Agilent Module to PC This chapter provides information on cables used with the 1290 series of HPLC modules. Agilent Technologies...

  • Page 114: Cable Overview

    Identifying Cables Cable Overview Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper N O T E functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Connector, Agilent module to 3394/6 integrators...
  • Page 115 It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m 1290 Infinity Flexible Cube User Manual...

  • Page 116: Analog Cables

    Shield Analog - Center Analog + Agilent Module to BNC Connector Connector, Agilent module to Pin BNC Pin Agilent Signal Name BNC connector module (p/n 8120-1840) Shield Shield Analog - Center Center Analog + 1290 Infinity Flexible Cube User Manual...
  • Page 117 Identifying Cables Analog Cables Agilent Module to General Purpose Connector, Agilent module to Pin 3394/6 Pin Agilent Signal Name general purpose module (p/n 01046-60105) Not connected Black Analog - Analog + 1290 Infinity Flexible Cube User Manual...

  • Page 118: Remote Cables

    Identifying Cables Remote Cables Remote Cables One end of these cables provides a Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Agilent Module to 3396A Integrators...
  • Page 119 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request 13, 15 Not connected 1290 Infinity Flexible Cube User Manual...
  • Page 120 3 - Gray Start 4 - Blue Shut down 5 - Pink Not connected 6 - Yellow Power on High 7 - Red Ready High 8 - Green Stop 9 - Black Start request 1290 Infinity Flexible Cube User Manual...

  • Page 121: Bcd Cables

    Black BCD 0 Orange BCD 3 BCD 2 Brown BCD 1 Gray Digital ground Gray Gray/pink BCD 11 Red/blue BCD 10 White/green BCD 9 Brown/green BCD 8 not connected not connected + 5 V 1290 Infinity Flexible Cube User Manual...
  • Page 122 Connector, Agilent module to Pin 3392/3 Pin Agilent Signal Name BCD Digit 3396 integrators module (p/n 03396-60560) BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V 1290 Infinity Flexible Cube User Manual...

  • Page 123: Can/Lan Cables

    CAN cable, Agilent module to module, 1 m LAN Cables Description 5023-0203 Cross-over network cable, shielded, 3 m (for point to point connection) 5023-0202 Twisted pair network cable, shielded, 7 m (for point to point connection) 1290 Infinity Flexible Cube User Manual...

  • Page 124: External Contact Cable

    EXT 2 Grey EXT 3 Pink EXT 3 Blue EXT 4 EXT 4 Black Not connected Violet Not connected Grey/pink Not connected Red/blue Not connected White/green Not connected Brown/green Not connected White/yellow Not connected 1290 Infinity Flexible Cube User Manual...

  • Page 125: Agilent Module To Pc

    It's also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9. 5181-1561 RS-232 cable, 8 m 1290 Infinity Flexible Cube User Manual...
  • Page 126 Identifying Cables Agilent Module to PC 1290 Infinity Flexible Cube User Manual...

  • Page 127: Appendix

    1290 Infinity Flexible Cube User Manual Appendix General Safety Information The Waste Electrical and Electronic Equipment (WEEE) Directive (2002/96/EC) Radio Interference Sound Emission Flow Cell Agilent Technologies on Internet This chapter provides addition information on safety, legal and web. Agilent Technologies...

  • Page 128: General Safety Information

    Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury. Whenever it is likely that the protection has been impaired, 1290 Infinity Flexible Cube User Manual...
  • Page 129 When working with solvents please observe appropriate safety procedures (e.g. goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet by the solvent vendor, especially when toxic or hazardous solvents are used. 1290 Infinity Flexible Cube User Manual...
  • Page 130 C A U T I O N alerts you to situations that could cause loss of data, or damage of equipment. ➔ Do not proceed beyond a caution until you have fully understood and met the indicated conditions. 1290 Infinity Flexible Cube User Manual...

  • Page 131: The Waste Electrical And Electronic Equipment (Weee) Directive (2002/96/Ec)

    “Monitoring and Control instrumentation” product. Do not dispose off in domestic household waste To return unwanted products, contact your local Agilent office, or see www.agilent.com for more information. 1290 Infinity Flexible Cube User Manual...

  • Page 132: Radio Interference

    If test and measurement equipment is operated with equipment unscreened cables and/or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises. 1290 Infinity Flexible Cube User Manual...

  • Page 133: Sound Emission

    This product has a sound pressure emission (at the operator position) < 70 dB. • Sound Pressure Lp < 70 dB (A) • At Operator Position • Normal Operation • According to ISO 7779:1988/EN 27779/1991 (Type Test) 1290 Infinity Flexible Cube User Manual...

  • Page 134: Flow Cell

    THF, dioxane, di-isopropylether) such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides, • Solvents containing strong complexing agents (e.g. EDTA), • Mixtures of carbon tetrachloride with 2-propanol or THF. 1290 Infinity Flexible Cube User Manual...

  • Page 135: Agilent Technologies On Internet

    Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: http://www.agilent.com Select Products/Chemical Analysis It will provide also the latest firmware of the modules for download. 1290 Infinity Flexible Cube User Manual...
  • Page 136 Diagnostic software flush pump inlet valve dimensions exchange cable frequency range analog electrical connections descriptions of external contact general error messages early maintenance feedback remote error messages RS-232 humidity fan failed cables error messages analog 1290 Infinity Flexible Cube User Manual...
  • Page 137 RS-232C 1290 Infinity Flexible Cube User Manual...
  • Page 138 Index unpacking user interface Flexible Cube valve rotor seal exchange valve rinse voltage range warnings and cautions weight 1290 Infinity Flexible Cube User Manual...
  • Page 140 In This Book This manual contains technical reference information about the Agilent 1290 Infinity Flexible Cube G4227A. • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • safety and related information.

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