Replacing An Excitation Filter, A Barrier Filter, And A Dichroic Mirror - Nikon ECLIPSE Ti2-U Instruction Manual

Inverted research microscope
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7.5.3

Replacing an Excitation Filter, a Barrier Filter, and a Dichroic Mirror

The excitation filter, barrier filter, and dichroic mirror in the filter cube can be removed and replaced with other
filters or mirrors.
Handling optical elements
When handling optical elements, wear gloves if possible, and do not touch the surface of the filter or the
mirror with your bare hands. Keep the optical elements free of dust, fingerprints, and stains.
Replacement of the fluorescent filter cube components
Follow the procedure below to replace the fluorescent filter cube components.
Replacing the excitation filter
The excitation filter is fixed to the filter cube with the screw-in type filter retaining ring.
When attaching a thin excitation filter, the spacer is required to be placed between the excitation filter and the
filter retaining ring.
• When thickness of filter is ≥6 mm and ≤7.2 mm:
• When thickness of filter is ≥4.5 mm and <6 mm:
• When thickness of filter is ≥3.5 mm and <4.5 mm:
1.
Turn the excitation filter retaining ring
counterclockwise and remove it.
2.
Replace the excitation filter with the desired
one. If the spacer is required, place the
spacer checking its attaching direction.
Then, fix the excitation filter (and the spacer
if required) by screwing the filter retaining
ring.
Excitation filter
Spacer (with its
front side facing
the retaining ring)
Filter
retaining ring
Replacement of the excitation filter
(Thickness of filter is ≥4.5 mm and <6 mm)
Attaching direction of the excitation filter
When attaching the excitation filter, check that the arrow on the filter ring points the dichroic mirror.
Attaching direction of the spacer
The attaching direction of the spacer differs depending
on the thickness of the excitation filter.
If the direction of the spacer is wrong, the filter cube
may collide with other components when attached.
• When thickness of filter is ≥4.5 mm and <6 mm
Place the spacer as its front side faces the retaining
ring.
• When thickness of filter is ≥3.5 mm and <4.5 mm
Place the spacer as its rear side faces the retaining
ring.
* When attaching a non-Nikon excitation filter, ask the manufacturer the attaching direction in advance.
C -F L
-C
U V - 2 A
E X 33
0- 38 0
D M 40
0
B A 41
0
131
Chapter 7 Assembly of the Devices
Spacer not required
Spacer required
Spacer required
Excitation filter
Filter
retaining ring
Replacement of the excitation filter
(Thickness of filter is ≥6 mm and ≤7.2 mm)
Excitation filter
Spacer (with its
front side facing
the retaining ring)
Filter
retaining ring
Replacement of the excitation filter
(Thickness of filter is ≥3.5 mm and <4.5 mm)
Rear side
Cross section of the spacer
C -F L
-C
U V - 2 A
E X 33
0- 38 0
D M 40
0
B A 41
0
C -F L
-C
U V - 2 A
E X 33
0- 38 0
D M 40
0
B A 41
0
Front side

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