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Light Reduction by Combined ND Filters of the Epi-fluorescence Attachment
Brightness
Improving the S/N ratio (shielding tube)
Exposure to excitation light from the Epi-fl attachment may cause the white LED to light up, resulting in a degradation of
the S/N ratio. When performing epi-fluorescence observations solely using epi-fluorescence microscopy, remove the
condenser and attach the provided shielding tube to the Epi-fl attachment, and/or place a plate on the field lens.
■
ND on HG precentered fiber illuminator
You can also adjust the light intensity using the ND on the HG precentered fiber illuminator.
For detailed information, refer to the operating manual provided with the HG precentered fiber illuminator.
Locating a target on the specimen
The standard procedure for epi-fluorescence microscopy is to first locate the target under differential interference contrast
or phase contrast microscopy, and then switch to epi-fluorescence microscopy.
To locate the target under the dia-illumination bright-field microscopy, you will need to note the following:
Under dia-illumination bright-field microscopy, start with a 10x objective, and adequately stop down the condenser.
Gradually increase the magnification. When the target becomes difficult to locate, switch to epi-fluorescence, and
use low excitation light.
You can also use other techniques, such as using the edge of the cover glass to approximate the position of the
target.
Switching Excitation Methods
17.1
Four filter cubes can be inserted into the CI-FL Epi-fluorescence
attachment. (→See Chapter 3, "6 Assembly for Epi-fluorescence
Microscopy")
Move the desired cube into the optical path by turning the filter
cube switching knob on the right side of the attachment.
For bright-field observations, leave one cube position empty, and
move this empty position into the optical path.
Use the filter cube motion restricting lever located at the upper
front section to limit the cube switching operation.
Six filter cubes can be inserted into the D-FL Epi-fluorescence
attachment. Refer to the D-FL Epi-fluorescence attachment
Instructions for details.
Chapter 2
ND4
1
-
1/4
○
1/8
-
1/16
-
1/32
○
1/64
○
1/128
-
1/512
○
(-: Removed from the optical path, ○: Placed into the optical path)
Individual Operations
ND8
-
-
○
-
○
-
○
○
1
Switching the filter cube
61
ND16
-
-
-
○
-
○
○
○
Filter cube
motion restricting
lever
CUBE
1
Filter cube
switching knob
2
3
4
Filter cube
nameplate window
Chap. 2
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