Azimuth Adjustment Of The Polarizer And Analyzer - Nikon Eclipse LV100POL Instructions Manual

19

Azimuth Adjustment of the Polarizer and Analyzer

For the diascopic observation
1 Remove the polarizer and analyzer from the optical path.
2 Focus on the specimen.
3 Pull out the analyzer slider and move the analyzer
into the optical path.
4 Turn the analyzer rotation dial and align at the "0"
position on the scale.
5 Turn the polarizer rotation ring and align at the "0"
position. Locate the polarizer for the diascopic
microscopy into the optical path and align its
orientation to the index.
6 Move the specimen out of the optical path.
7 Move the Bertrand lens into the optical path.
The pupil of the objective will then be visible
through the eyepiece.
8 Turn the polarizer rotation ring for the diascopic
microscopy and adjust the orientation so that the dark
cross image appears in the pupil as shown in the right
figure.
This is so-called the crossed Nicols position, where
the orientations of the polarizer and analyzer coincide
with those of the orientation plate on the microscope
base (the polarizer, P, is in the X direction and the
analyzer, A, is in the Y direction).
It should be noted that the X direction is explained as that of the analyzer and Y direction as
that of the polarizer in some commercially available technical manuals and reference books.
For the episcopic observation
1 Push in the analyzer setting knob and move the
analyzer out of the optical path.
2 Place a dummy specimen on the stage. It must have
high reflectance with optical isotropy, for example
a mirror. And then, focus on the specimen.
3 Pull out the analyzer slider and move the analyzer
into the optical path.
4 Turn the analyzer rotation dial and align at the "0"
position on the scale.
5 Push in the polarizer slider on the epi illuminator to
place the polarizer for the episcopic microscopy into
the optical path.
6 Move the Bertrand lens into the optical path.
7 Turn the polarizer rotation ring for the episcopic
microscopy and adjust the orientation so that the dark
cross image appears in the pupil as shown in the right
figure.
When the dark cross can be seen, the condition is
called the crossed Nicols.
38
0
Move the
Bertrand
lens into
the optical
path.
Rotate the
0. 8 Ach r
0.6 0.90
0.4 JAPAN
0.2
polarizer
rotation
ring.
F.S.
dark-cross
Analyzer
scale: 0
0
Move the
Bertrand
BF
D F
lens into
the optical
path.
Rotate the polarizer rotation ring.
Ach r
Polarizer orientation 0. 8 0.90
0.6 JAPAN
0.4 0.2
Lateral
direction
F.S.
dark-cross
Analyzer
scale: 0
NC B
ND 8
P
A. ST O
F. ST O P
Vertical
direction
NC B
ND 8