Reflected Light Brightfield/Darkfield Observation Procedure - Olympus BXFMA Instructions Manual

REFLECTED LIGHT BRIGHTFIELD/DARKFIELD
OBSERVATION PROCEDURE
}The following flow chart pertains to the reflected light brightfield/darkfield observations.
The controls used for a system using the U-HSTR2 hand switch, U-FH focus adjustment knob unit and TH4 power supply
unit for halogen bulb together with those used for a PC-controlled system.
For details on the PC control, also refer to the tutorial or help provided with the BX2-BSW software.
Set the main switch to " I " (ON).
Select BF (brightfield) or DF
(darkfield).
Select the light path (when a trin-
ocular tube is used).
Place the specimen on the speci-
men table.
Engage the 10X objective in the
light path.
Bring the specimen in focus.
Adjust the aperture iris and field
iris diaphragms.
}In DF observation, both should
be opened fully.
Engage the objective to be used
in the light path and bring the
specimen in focus.
Start observation.
8
Adjust the brightness.
Adjust the interpupil-
lary distance.
Adjust the diopter.
Engage the required
filters.
Adjust the brightness.
(Non PC Controlled System)
1BX-UCB main switch " I " (ON)
2TH4 main switch " I " (ON)
4BF/DF button (Page 10)
5Light path selector knob (Page 15)
6Revolving nosepiece button
7Objective Up/Down buttons, or
8Focus adjustment knob
9Light intensity adjustment knob
aBinocular sleeves (Page 14)
bDiopter adjustment ring (Page 14)
cAS+/– buttons (Page 11)
dField iris diaphragm auxiliary
lever (Page 11)
6Revolving nosepiece button
7Objective Up/Down buttons or
8Focus adjustment knob
eFilter insertion slot (Page 13)
9Light intensity adjustment knob
(PC Controlled System)
1Main switch " I " (ON)
3PC " I " (ON)
3PC command
5Light path selector knob (Page 15)
3PC command
3PC command
3PC command
aBinocular sleeves (Page 14)
bDiopter adjustment ring (Page 14)
3PC command
dField iris diaphragm auxiliary
lever (Page 11)
3PC command
3PC command
eFilter insertion slot (Page 13)
3PC command