Reflected Light Fluorescence Observation Procedures - Olympus BXFM Instructions Manual

System microscope
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4-4 Reflected light fluorescence observation procedures

1
Turn ON the main switch.
2
Select the light path. (Trinocular tube only)
3
Place a sample on the stand.
4
Engage the 10X objective in the light path.
5
Engage the fluorescence mirror unit
suitable for the sample in the light path.
6
Open the shutter.
7
Bring the sample into focus.
11
Open the aperture diaphragm, and
adjust the field diaphragm.
12
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Start observation.
8
Adjust the brightness.
9
Adjust the interpupillary distance.
10
Adjust the diopter.
13
Adjust the brightness.
Main switch of power supply
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Turret of the reflected light illuminator (page 16)
Shutter of the reflected light illuminator
Coarse / Fine focusing knob of the BXFM
frame
Brightness control knob of the power supply
Binocular tube
Diopter adjustment ring
Aperture diaphragm knob of the reflected
light illuminator
Field diaphragm knob of the reflected light
illuminator
Nosepiece
Coarse / Fine focusing knob of the BXFM
frame
Brightness control knob of the power supply
BXFM
(page 16)
(page 18)
(page 18)
(page 18)
(page 19)
(page 46)
(page 20)
(page 21)
(page 23)
(page 23)
(page 30)
(page 27)
(page 19)
(page 20)
(page 21)
39

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