Reflected Light Fluorescence Observation Procedures - Olympus BX53M Instructions Manual

4-4 Reflected light fluorescence observation procedures

1
Turn ON the main switch.
2
Select the reflected light illumination
3
Select the light path. (Trinocular tube only)
4
Place a sample on the stage.
5
Engage the 10X objective in the light path.
6 Engage the fluorescence mirror unit
suitable for the sample in the light path.
7 Open the shutter.
8 Bring the sample into focus.
12
Open the aperture diaphragm, and
adjust the field diaphragm.
13
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
Start observation.
9 Adjust the brightness.
10
Adjust the interpupillary distance.
11 Adjust the diopter.
14
Adjust the brightness.
Main switch
Transmitted/reflected light switch
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Turret of the reflected light illuminator (page 17)
Shutter of the reflected light illuminator
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
Binocular tube
Diopter adjustment ring
Aperture diaphragm knob of the reflected
light illuminator
Field diaphragm knob of the reflected light
illuminator
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
BX53M
(page 16)
(page 16)
(page 19)
(page 19)
(page 19)
(page 23)
(page 64)
(page 24)
(page 26)
(page 32)
(page 32)
(page 39)
(page 36)
(page 23)
(page 24)
(page 26)
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