Optimizing Performance; General Guidelines For A Microscale System; Required Solvents - Waters ACQUITY UPLC M-Class System Manual

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Optimizing Performance

Optimal performance of an ACQUITY UPLC M-Class system depends
on a thorough understanding of the effects of dispersion and carryover
as well as leak-prevention measures and sample preparation
techniques.
Contents:
Topic

General guidelines for a microscale system ................................... 25

Dispersion......................................................................................... 27
Carryover.......................................................................................... 27
Contamination.................................................................................. 28
Preventing leaks .............................................................................. 29
Sample preparation ......................................................................... 30
General guidelines for a microscale system
Separations performed on the ACQUITY UPLC M-Class System use smaller
ID columns when compared to an analytical UPLC system. Solvent and
sample consumption are therefore significantly reduced.
The microscale chromatograph requires optimal performance from the
μSM-FL because sample dispersion is more evident when using smaller
columns.
Recommendation:
be less than 0.5 seconds wide. For good quantitation, a sampling rate of 10 to
15 points across a peak is desirable.

Required solvents

Use only high-quality solvents.
Important:
Appendix D, for additional solvent considerations.
See also:
Regarding fast, microscale analyses, a peak of interest can
General guidelines for a microscale system
March 10, 2014, 715003588 Rev. A
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