Agilent Technologies 6850 Series II User Information page 171

Manual injection with septum nut
Before making your injection, make sure the correct septum nut and septum
are installed.
1.
2.
3.
4.
5.
6.
These steps should be done smoothly, with minimal delay.
Manual injection with cooling tower
When injecting with fused silica or metal removable stainless steel needles, be
sure the cooling tower assembly and duckbill are installed on the inlet. Initial
pressure must be set at less than 207 kPa (30 psi). Higher pressures will make
needle insertion difficult.
1.
2.
3.
4.
Warning The cooling tower may be hot!
5.
Released: March 2004
Immerse the syringe needle in sample; pump the syringe plunger to expel
air from the barrel and needle.
Draw the sample into the syringe.
Remove the needle from the sample and draw about 1 µL of air into the
syringe.
Wipe the needle dry if it is wet.
Guide the needle straight into the septum nut, pierce the septum, and
insert the needle fully into the inlet until it bottoms.
Start the run, depress the syringe plunger as quickly as possible, and
withdraw the needle from the inlet.
Immerse the syringe needle in the sample and pump the syringe plunger to
expel air from the barrel and needle.
Draw the sample into the syringe. Allow enough time for fluids to pass
through the small bore of the needle.
Remove the needle from the sample and draw about 1 µL of air into the
syringe. Wipe the needle with a tissue wetted with solvent.
Press down the top of the cooling tower with a pencil to open the duckbill.
Hold down the cooling tower and guide the needle until it is fully inserted
in the inlet. You may observe a drop in the pressure reading on the control
table.
If the needle does not go in all the way, try rotating the syringe and slightly
releasing pressure on the cooling tower.
If you still cannot get the needle in, the duckbill opening may be stuck. Try
removing the duckbill, opening it manually, and reinstalling it.
6850 Series II GC User Information
Routine Maintenance: Inlets
Maintaining a cool on-column inlet
page 171 of 256