Table of Contents
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This manual applies to OpenLAB CDS ChemStation Edition Software (version
C.01.03 and later until superseded) and Agilent 6100 Series LC/MS
instruments.
Where to find more information
Online Help Use online Help for in-depth information beyond what is shown
in this Quick Start Guide. Display online Help in any of the following ways:
• Click the Help button in the toolbar.
• Select Help Topics from the Help menu.
• Click the Help button on most dialog boxes to show task-specific help.
Agilent 6100 Series Quadrupole LC/MS
Systems
Quick Start Guide
Where to find more information
Configuration Diagrams
ChemStation Views
5
Basic Operation
18
Deconvolution
23
Operating Tips
25
Troubleshooting Tips
1
3
27
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Table of Contents
Related Manuals for Agilent Technologies 6100 Series LC/MS
Summary of Contents for Agilent Technologies 6100 Series LC/MS
- Page 1 Operating Tips Troubleshooting Tips This manual applies to OpenLAB CDS ChemStation Edition Software (version C.01.03 and later until superseded) and Agilent 6100 Series LC/MS instruments. Where to find more information Online Help Use online Help for in-depth information beyond what is shown in this Quick Start Guide.
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Page 2: What's New In
• Support for ESI with Agilent Jet Stream Technology on G6130B (hardware upgrade required). What's New in C.01.02? • Support for Windows 7 32-Bit and 64-Bit. • Support for 6100 Series LC/MS on OpenLAB CDS ChemStation Edition. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 3: Configuration Diagrams
Configuration Diagrams 1100/1200/1260/1290 Series LC Configuration Diagrams 1100/1200/1260/1290 Series LC Solvent bottles Solvent cabinet Vacuum degasser Pump Control module (optional) Autosampler Column compartment LC Detector (DAD or VWD) Agilent 6100 Series LC/MS System Quick Start Guide... - Page 4 Configuration Diagrams LC Cabling LC Cabling Remote cable CAN bus cable CAN bus cable AC power Analog signal to recorder Shielded LAN cable to network hub Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 5: Chemstation Views
MSD Tune View In this view, you can calibrate your LC/MS automatically. You can also set MS parameters manually for specific types of molecules. See page 17 for more information. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 6: Method And Run Control View
Help and these manuals on the ChemStation disk: • Agilent 6100 Series Single Quad LC/MS Systems Quick Start Guide • Agilent 6100 Series Quadrupole LC/MS Systems Familiarization Guide • Agilent 6100 Series Quadrupole LC/MS Systems Concepts Guide Agilent 6100 Series LC/MS System Quick Start Guide... - Page 7 Each icon of the system diagram represents one component or module of your system. Click on an icon if you want to edit the method parameters or go to the online Help for that particular component. Injector Pump & Solvents Column Detectors Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 8 To enable FIA from the system diagram: • Click the FIA icon. • Select Edit FIA Series. • Mark the Enable FIA Series in Method option and edit the FIA Table. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 9: Data Analysis View
LC/MS Signal Message Line The various tool sets available in Data Analysis View are shown on the following pages. You can view a description of a button by moving the cursor over it. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 10 Common Tool Set This toolset is always present in Data Analysis view. Graphics Tool Set Allows you to manipulate the graphic display. Integration Tool Set Allows you to perform integration and reporting tasks on a chromatogram. Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 11 Purify Tool Set Allows you to work graphically with your purification data. Spectral Tool Set Allows you to perform spectral evaluation tasks. Deconvolution Tool Set Allows you to transforms mass spectra from multiply-charged ions into a calculated molecular weight. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 12: Report Layout View
• Select File / Add to Report Styles to add your completed report template to the list of available report styles. Now you can use your customized report within a method. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 13 Uses the same elements as the standard ESTD reports so you can customize your external standard reports. AREAPCT.FRP Uses the same elements as the standard area percent report so you can customize your area percent reports. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 14: Verification (Oq/Pv) View
ChemStation software are operating correctly according to predefined performance criteria. Operation Qualification (OQ) Operation Qualification is the documented verification that the equipment-related system or subsystem performs as intended throughout representative or anticipated operating ranges. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 15 Help for more information on these tests. • VWD Wavelength Accuracy • Intensity • Holmium • Temperature Accuracy • Noise, Flow, Temperature • DAD Wavelength Accuracy • Injector Precision • Detector Linearity/Carry-over • Injector Linearity • Gradient Composition Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 16: Diagnosis View
This view allows you to carry out diagnostic and maintenance activities on your system. Diagnosis Toolbar The Diagnosis toolbar is displayed when you select Show Top Toolbar from the View menu. Move the cursor over button to view a description of it. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 17: Msd Tune View
Change spray chamber parameters. Generate a profile and scan Edit LC/MS parameters for report. manual tuning. Autotune the LC/MS and print a Acquire multiple scans and send tune report. results to data file. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 18: Basic Operation
• the MS stream selection valve is standby mode (indicated by the gray set LC to Waste. color in the system diagram). For Agilent Jet Stream source: • Sheath temp 100°C • Sheath gas flow 2.2 L/minute Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 19: Tune The Lc/Ms
Use autotune for automated Autotune from the Tune menu or click adjustment of the LC/MS the Autotune toolbar button. performance. 2 Review the tune report that is printed automatically when tuning is completed. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 20 5 Adjust the signal strength (setting iris and adjusting the multiplier gain). Fragmentor and gain are method parameters. The fragmentor affects ion transmission and fragmentation. For more information, see the online Help. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 21: Acq Uire Lc/Ms Data
• Samples can be injected either manually or with an ALS. • Always start a run from the software. • An FIA method cannot be used in a sequence but multiple FIA methods can be run sequentially. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 22 10 When you are ready to begin a run, click the Start button. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 23: To Deconvolute A Mass Spectrum
7 Click the Run Deconvolution button to begin Once the deconvolution is done, the the deconvolution process. components are displayed in the upper right window. The charge states are displayed in the lower right window. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 24: Deconvolution Report
Gaussian curve perfectly, due to other errors such as errors in mass axis assignment or unresolved chemical impurities. The Deconvolution software is optional and is part of the OpenLAB CDS ChemStation C.01.xx or M8363AA (ChemStation C.0x) Bioanalysis Software package. Agilent 6100 Series LC/MS System Quick Start Guide... -
Page 25: Operating Tips
• Use the following table as a guide to using SIM, condensed scan, and full scan acquisition modes. Task Mode Acquire electrospray data for samples containing large, Full Scan multiply-charged analytes. Analyze a mixture with unknown components (small molecules). Scan Agilent 6100 Series LC/MS System Quick Start Guide... - Page 26 Identify the presence of a few known compounds at low levels within a mixture. Use fast chromatography on 1.0-MHz LC/MS systems (G6120B, Fast Scan G6130B) without missing peaks. Use fast chromatography on 1.4-MHz LC/MS systems (G6150B) Ultra Fast Scan without missing peaks. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 27: Troubleshooting Tips
Verify that the sample is fresh and has been stored correctly. ✔ Verify that the MS system is tuned correctly. ✔ Check the nebulizer condition. ✔ Clean the capillary entrance. ✔ Check the capillary for damage and contamination. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 28 Troubleshooting Tips ✔ Verify that the spray chamber parameters are set correctly, such as drying gas heater and nebulizer pressure. Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 29 Check the position of the needle in the nebulizer. ✔ Stop the solvent flow and remove the nebulizer assembly. Use a magnifying glass to examine the end of the nebulizer for damage. Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 30 Verify that the MS stream selector valve is set to LC to MSD. Undesired fragmentation ✔ Fragmentor is set too high. ✔ Ionization is causing fragmentation (APCI vs. Electrospray). ✔ APCI temperature is too high. Agilent 6100 Series LC/MS System Quick Start Guide...
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Page 31: Safety Warnings
Do not interrupt the protective conductor inside or outside the LC/MS or disconnect WARNING the protective earth terminal. You can create a shock hazard for the operator and can damage the instrument. Agilent 6100 Series LC/MS System Quick Start Guide... - Page 32 Before you perform maintenance on the electronics vent and turn off the LC/MS WARNING and disconnect the power cord. The power switch on the front of the LC/MS does not completely cut power to the instrument. Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 33 Safety Warnings Agilent 6100 Series LC/MS System Quick Start Guide...
- Page 34 Where to find more information • What’s New in C.01.06? • Configuration Diagrams • ChemStation Views • Basic Operation • Operating Tips • Troubleshooting Tips • Safety Warnings Agilent Technologies, Inc. 2014 Printed in USA Revision A, March 2014 *G1960-90082* G1960-90082...