User Manuals: Biotek EL800NB Microplate Reader

Manuals and User Guides for Biotek EL800NB Microplate Reader. We have 1 Biotek EL800NB Microplate Reader manual available for free PDF download: Operator's Manual

Biotek EL800NB Operator's Manual

Biotek EL800NB Operator's Manual (218 pages)

Brand: Biotek | Category: Laboratory Equipment | Size: 2 MB

Table of Contents

All Rights Reserved
6
Restrictions and Liabilities
6
Trademarks
6
Table of Contents
7
Contact Information
14
Customer Service and Sales
14
European Coordination Center
14
Service/Tac
14
Document Conventions
15
Revision History
16
Intended Use Statement
19
Quality Control
19
Repackaging and Shipping
19
Hazards
20
Hazards and Precautions
20
Warnings
20
Precautions
21
Directive 2002/96/EC: Waste Electrical and Electronic Equipment
23
Directive 73/23/EEC Low Voltage
23
Directive 89/336/Eec:electromagnetic Compatibility
23
Directive 98/79/EC: in Vitro Diagnostics
23
Electromagnetic Interference and Susceptibility
24
User Safety
24
Safety Symbols
25
Chapter 1: Introduction

27
- Introducing the Elx800 Absorbance Microplate Reader
  28
- Hardware Features
  29
- Software Features
  29
- Package Contents
  30
- Optional Accessories
  31
- Specifications
  32
- Standard Model
  32
- Ultraviolet/Uv Model
  33
- Narrow Beam/Nb Model
  34
- Product Support & Service
  36
- Contacting the Technical Assistance Center
  36
- Returning Instruments for Service/Repair
  36
Chapter 2: Installation

37
Chapter 3: Operation

63
- Elx800 Front Panel
  64
- Figure 14: Keyboard
  64
- Overview
  66
- Recommendations for Achieving Optimum Performance
  66
- System Startup
  67
- Main Menu
  68
- Figure 15: Options Available from the Main Menu
  68
- Define
  70
- Programming Note
  71
- Selecting an Assay to Define
  72
- Editing the Assay Name
  72
- Define (Method, Map, Formula and Curve)
  73
- Defining METHOD
  73
- Defining MAP
  77
- Defining FORMULA
  93
- Defining CURVE
  105
- Reading a Microplate
  112
- Selecting an Assay to Run
  113
- Printing Reports
  117
- Editing Standard Outliers
  118
- Printing Results
  120
- Using the Utility Options
  121
- Setting the Date and Time
  121
- Viewing/Editing the Filter Table
  122
- Specifying Data Output and Reporting Options
  123
- Selecting Read Options
  125
Chapter 4: Instrument Qualification

127
- Table 1: Recommended Qualification Schedule
  127
- Recommendations for Achieving Optimum Performance
  128
- Recommended Qualification Schedule
  129
- Qualification Procedures
  130
- System Test
  131
- Checksum Test
  133
- Absorbance Plate Test
  134
- Description
  134
- Requirements
  136
- Figure 18: Sample Standards Certificate for the Absorbance Test Plate
  136
- Defining the Absorbance Test Plate Parameters
  137
- Running the Absorbance Plate Test
  138
- Test Failures
  139
- Table 2: Typical Enzyme-Substrate Combinations and Stopping Solutions
  139
- Liquid Testing
  140
- Stock Solution Formulation
  141
- Liquid Test 1
  143
- Table 3: Test Tube Dilutions
  144
- Liquid Test 2
  145
- Liquid Test 3 (for "UV" Models Only)
  149
- Table 4: PBS 10X Concentrate Solution
  149
Chapter 5: Maintenance and Decontamination

153
- Maintenance
  154
- Routine Cleaning Procedure
  154
- Purpose
  154
- Tools and Supplies
  155
- Procedure
  155
- Filter Storage and Handling
  155
- Replacing the Bulb
  156
- Figure 20: Lamp Assembly
  157
- Decontamination Procedure
  159
- Tools and Supplies
  160
- Procedure
  160
Chapter 6: Troubleshooting and Error Codes

163
- Overview
  164
- System Test Description
  164
- Glossary of Terms
  164
- Error Codes
  165
- General Errors
  166
- Fatal Errors
  172
Appendix A: Computer Control

173
- Overview
  174
- Controlling the Reader with Gen5
  175
- Setting up Gen5
  175
- Problems
  176
- Getting Started with Gen5
  176
- Controlling the Reader with KC4
  178
- Setting up KC4
  178
- Problems
  179
- Getting Started with KC4
  179
- Controlling the Reader with Kcjunior
  180
- Setting up Kcjunior
  180
- Problems
  181
- Getting Started with Kcjunior
  181
Appendix B: Using 384-Well Geometry

183
- Perform a 384-Well Plate Read in Gen5
  184
- Figure 21: Reader Configuration Dialog
  184
- Figure 22: Reader Settings Dialog
  184
- Figure 23: New Experiment Dialog
  185
- Figure 24: Experiment Workspace
  185
- Figure 25: Procedure Dialog
  186
- Figure 26: Read Step Dialog
  186
- Figure 27: Plate Reading Dialog
  187
- Perform a 384-Well Plate Read in KC4
  188
- Figure 28: KC4 Main Menu, System|Readers
  188
- Figure 29: Reader Selection Dialog
  188
- Figure 30: New Data File Dialog
  189
- Figure 31: Reading Dialog
  189
- Figure 32: Plate Reading Dialog
  190
- Perform a 384-Well Plate Read in Kcjunior
  191
- Figure 33: Setup|Reader Dialog
  191
- Figure 34: Reader Setup Dialog
  191
- Figure 35: Read Plate Dialog
  192
- Figure 36: Protocol Definition Dialog
  192
- Figure 37: Example Read Method Parameters
  193
Appendix C: Report Format

195
Appendix D: Comparison of the Elx800 and the EL800

203
Appendix E: Instructions for Programming a New Assay

205
- Sample ANA Screen Enzyme Immunoassay Kit
  206
- Intended Use
  206
- Background
  206
- Principle of the Assay
  206
- Materials
  207
- Quality Control and Results
  208
- Expected Values
  208
- Programming the ANA Screen Enzyme Immunoassay Kit
  209
- Sample Human Anticardiolipin Igg Enzyme Immunoassay Kit
  211
- Intended Use
  211
- Background
  211
- Principle of the Assay
  211
- Materials
  212
- Quality Control and Results
  213
- Expected Values
  215
- Programming the Human Anticardiolipin Igg Enzyme Immunoassay Kit
  216
- Programming the Human Anticardiolipin Igg
  216
- Enzyme Immunoassay Kit
  216

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