Epi-Fluorescence Microscopy Procedure - Nikon Eclipse Ni-E Instruction Manual

4

Epi-fluorescence Microscopy Procedure

This section describes the epi-fluorescence microscopy procedure which is performed using the microscope's controls
and buttons.
Precautions when switching from epi-fluorescence microscopy to differential interference contrast microscopy or phase
contrast microscopy are also described.
Find the target in the specimen with dia-illumination as described in "3 Bright/Dark-field Microscopy Procedure" and then
prepare for epi-fluorescence microscopy.
The microscope can also be controlled from DS-L3 if a DS-L3 DS Camera Control Unit is connected to the microscope.
Examples of DS-L3's [MICROSCOPE CONTROL] screen with the used control buttons are shown for procedures that can
be performed from DS-L3. The content and layout of buttons on the screens in the figure and the actual [MICROSCOPE
CONTROL] screen may differ because the setting of buttons on the [MICROSCOPE CONTROL] screen can be changed
as desired.
For details on the control from DS-L3, see Chapter 3 "23 Operation on DS-L3" - "23.2 Controlling the Microscope" in this
manual.
WARNING
The light source used with the epi-fluorescence attachment (mercury lamp) requires special care during handling because of
its characteristics. Make sure you are familiar with and adhere to all warnings and cautions described at the beginning of this
instruction manual.
Preparation
Find the target in the specimen with dia-illumination as described in "3 Bright/Dark-field Microscopy". (See Chapter 3 "16.9
Other Notes on Epi-fluorescence Microscopy".)
1
Turn off the dia-illumination lamp.
Press the dia-illumination ON/OFF switch.
Chapter 2
Microscopy Operations
Preparation
Epi-fluorescence
Microscopy Procedure
1 2 3 4 5
33
Focusing Microscopy
6 7 8 9 10 11 12 13 14 ■ ■ ■ ■ ■
Dia-illumination lamp OFF
P F _ 1 0 x _ _ _ _
D I A : O F F _ _ _ _ _ _ _ _ _
[MICROSCOPE CONTROL] screen
[LAMP ON/OFF] button: Lamp OFF
Z : _ _ _ _ _ 0 . 0 0 0 u m
F S 3 0 . 0 m m