Kohler Illumination - Nikon A1+ User Manual

Keep image as multchannel- Results in a multiplane tiff file where each frame of the
stack is a channel and displayed in grey scale. The image contains the raw data if the
keep bit depth option has also been selected

Kohler Illumination

1. Select the brightfield optical config
2. Ensure the condenser aperture turret is set to the "A" position
3. Focus the sample
4. Open the condenser aperture fully
5. Close down the field aperture fully
6. If the image of the field aperture is not in sharp focus, adjust the focus
by moving the condenser up/down
7. Centre the image of the field aperture using the centring screws
8. Open the field aperture until you can see the full field of view but no
more
9. Remove an eyepiece and look down, if you open and close the condenser aperture
you can now see its image
10. Close the condenser aperture until ~70% of the back illuminated
area is filled
11. Reinsert the eyepiece
12.
Kohler should be adjusted whenever you change the lens
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