Locking The Focus Position - Nikon ECLIPSE E400 Instructions Manual

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Coarse focus stopper
Once the coarse focus stopper is clamped in place, the coarse focus knob cannot be used to move the stage any higher. (Movement
of the stage by the fine focus knob is not restricted.)
In effect, once the coarse focus knob is clamped in place at the focus
position, a rough focus can be attained the next time simply by turning the coarse focus knob as far as it will go.
This feature is convenient when viewing similar specimens one after another.
Using the coarse focus stopper
With the specimen in focus, turn the coarse focus stopper ring as
far as it will go (about 270°) in the arrow direction indicated on
the microscope base (i.e., clockwise direction). The coarse focus
stopper is now clamped in place.
When changing the specimen, tower the stage by turning only
the coarse focus knob.
After changing the specimen, gently raise the stage by turning
only the coarse focus knob as far as it will go.
The specimen should be roughly in focus when the stage has
been raised as far as it will go; use the fine focus knob to bring
the specimen into perfect focus.
If the coarse focus stopper is not being used, be sure to loosen
the coarse focus stopper ring as far as it will go (by turning it as
far as it will go in the opposite direction of the arrow indicated
on the microscope base).
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