Perfect Focus System (Pfs) Operation - Nikon A1+ User Manual

Perfect Focus System (PFS) Operation

PFS provides real time focus correction on a plane of interest during time lapse
experiments carried out above room temperature. PFS is required because the
microscope components expand as they heat which can cause the focus to drift. The
coverslip/medium interface is tracked and the distance from the interface to the plane of
focus is recorded as an offset value. If a change in the distance from the coverslip
interface is detected, the lens will move in the same direction to compensate, this
maintains the same offset value and therefore the focal plane is unchanged.
1. Focus on the sample
2. On the front of the microscope a green LED will
light next to the word "Focus" if the PFS can be
turned on. This indicates the refractive index
interface has been found.
3. Press the On button the front of the microscope
to enable PFS
4. Check the focus again now PFS has been
turned on, you may have to adjust it again
5. To change focus while PFS is enabled, use the PFS offset
controller wheel
6. The blue button on the side of the controller toggles between
course/fine focus
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