Operation Flowchart - Nikon SMZ25 Instruction Manual

1

Operation Flowchart

Microscopy under episcopic illumination or
diascopic illumination
1
Turn on the power.
2
Set a sample.
Bring an objective into the optical
3
path.
Turn on the illumination and adjust
4
the brightness.
Set the optical path to 100% to the
5
binocular part.
Adjust the depression angle of the
6
tilting tube.
7
Adjust the diopter.
8
Adjust the interpupillary.
9
Adjust the focus.
Change the magnification using the
10
zoom knobs.
Turn off the power after completing
11
microscopy.
* In microscopy under diascopic
illumination using an epi-fluorescence
attachment, attach an empty cube
(supplied with the microscope) to the
epi-fluorescence attachment and bring it
into the optical path.
* When using a compact system of an
SMZ18 Zooming Body combined with a
P-PS32 Plain Stand and P2-FMDN Focus
Mount without using an illumination
system, perform the steps above in the
order of [2], [5], [6], [7], [8], [9], and [10].
Chapter 2
Microscopy Procedures
11
12
13
14
15
16
17
8
Epi-fluorescence microscopy
After locating the target in the sample with
microscopy under diascopic illumination, follow
the procedure below:
Turn off the diascopic LED illumination.
Bring the filter cube into the optical path.
Adjust the angle of the light shielding plate.
Turn on the HG precentered fiber illuminator and
adjust the brightness.
Adjust the focus.
Change the magnification by zooming in and out.
Turn off the power after completing microscopy.