Oil-Immersion Operation - Nikon ECLIPSE E600 Instructions Manual

LU oil-immersion operation
o (using an oil-immersion type of objective and condenser)
An objective marked "Oil" is an oil-immersion type. These objectives are used with the immersion
oil applied between the specimen and the tip of the objective. The immersion oil is provided for the
microscope.
To get maximum performance from an oil-immersion type of objective with a numerical aperture of
1.0 or greater, an oil-immersion type of achromat/aplanat condenser must also be used. These con-
densers are used with oil applied between the specimen and the condenser lens.
Bubbles in the oil will adversely affect the viewing of the image, so be careful to prevent the forma-
tion of air bubbles. To check for air bubbles, remove the eyepieces, fully open the field and aperture
diaphragms, and look at the exit pupil of the objective within the eyepiece tube. (The exit pupil will
appear as a bright circle.) When it is difficult to see if there are any bubbles, mount a centering
telescope (sold separately) on the eyepiece sleeve with an adapter (sold separately). Then, while
turning the eyepiece on the centering telescope to change the focus, look through the centering
telescope for air bubbles. If there are bubbles in the oil, remove them by one of the following
methods:
» Turn the revolving nosepiece slightly, moving the oil-immersed objective back and forth once
or twice. (In the case of the condenser, gently turn the condenser focus knob to move the
condenser up and down slightly.)
* Add more oil.
* Remove the oil and replace it with new oil.
Use as little oil as possible (just enough to fill the space between the tip of the objective and the
specimen, or between the tip of the condenser and the specimen). If too much oil is applied, the
excess will flow onto the stage and around the condenser.
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