Waters ACQUITY UPLC M-Class System Manual page 52

4 Maintaining the system
ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Baseline or
background
fluctuations
(continued)
52 March 10, 2014, 715003588 Rev. A
Suspected cause
Inadequate solvent
blending
NanoFlow sprayer/emitter
problem
Increased background
caused by possible:
• Contamination
• Dirty source
• Detector setting too
high
• Electronic noise
• Column problem
Reduced background noise
caused by possible:
• Detector setting too low
• Loss of sensitivity
Possible solution
If the baseline is steady
when running a pre-mixed
solvent then:
• Ensure that A and B
pumps are delivering
solvent correctly.
• For shallow gradients
or protein applications
running at lower
pressure switch to the
mixer kit for the 1-mm
column.
Clean or replace the
emitter.
The Universal
See also:
NanoFlow Sprayer
Installation and
Maintenance Guide on the
documentation CD.
Ensure cleanliness of
solvents and proper use of
column. Adjust the
detector setting and refer
to the appropriate
documentation for that
instrument.
Adjust the detector setting
and refer to appropriate
documentation for that
instrument.