Waters ACQUITY UPLC M-Class System Manual page 26

2 Optimizing Performance
Requirement:
• Use high-quality solvents, buffers, and additives (LC/MS-grade,
prefiltered).
• Use high-quality water (LC/MS-grade).
Follow these general recommendations when performing a microscale
analysis:
• Always use solvent filters on tubing lines in solvent bottles.
• Filter salt-containing buffers with a 0.2-μm filter membrane.
• Keep concentrated stock solutions to use when you prepare working
solutions.
• Make fresh buffer solutions daily.
• Do not add fresh buffer to old buffer, which can promote microbial
growth.
• Keep the seal-wash line primed.
• Prime solvent lines during system startup.
• Keep all solvent lines primed.
• Do not block the degasser vent line. Trim the tubing, if necessary.
Eluent lines A1 and B1 do not pass through the degassers. When
Note:
working at flow rates less than 1μL/min, passing the eluent through the
degasser may cause depletion of any volatile mobile phase modifier. For
most applications, a Waters column packed with sub-2-micron particles
provides sufficient back pressure to retard outgassing. If outgassing
occurs. Waters recommends use of the optional solvent stabilization kit.
• Do not submerge the waste or degasser vent lines in liquid.
See also:
Information for details on how to route waste and degasser tubing.
• Flush buffers from the system, using water, immediately after you are
done with them.
• Use 10 to 20% organic solvent in water as a storage solvent if you keep
the system idle for extended periods (longer than 24 hours).
• If the system is idle for a day or two keep the system running rather
than shutting down.
26 March 10, 2014, 715003588 Rev. A
The μ Binary Solvent Manager Overview and Maintenance