Waters ACQUITY UPLC M-Class System Manual page 101

Warning:
when moving the micro cart or instrument modules or when
adding or changing reagents.
Solvent manager recommendations
• The seal wash system must never run dry, particularly during
separations that use a polar mobile phase.
• For reversed-phase applications, use aqueous seal wash solutions with a
weak organic component (for example, 1:9 methanol/water).
• Do not use nonvolatile buffers as seal wash solvents.
• Ensure that the mobile phase is completely soluble in and compatible
with all of the solvents in use on the system.
μSM-FL recommendations
• Typical organic sample diluents such as dimethylsulfoxide (DMSO) and
dimethylformamide (DMF) are supported.
• Do not use buffers as needle wash solvents. You can use acids and bases.
Properties of common solvents
The following table lists the properties for some common chromatographic
solvents.
Properties of common solvents:
Solvent
Acetonitrile
n-butyl acetate
n-butyl alcohol
n-butyl chloride
Chlorobenzene
To prevent injury, always remove the solvent bottles
Vapor Pressure
mm Hg (Torr)
88.8 at 25 °C
7.8 at 20 °C
4.4 at 20 °C
80.1 at 20 °C
8.8 at 20 °C
Properties of common solvents
Boiling
Point (°C)
81.6
126.11
117.5
78.44
131.69
March 10, 2014, 715003588 Rev. A
Flash
Point (°C)
6
22
37
-9
28
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