Waters ACQUITY UPLC M-Class System Manual page 99

using a buffer, flush it from the pump by running a wet-prime with at least
five system volumes of HPLC-grade, distilled or deionized water.
To prevent microbial growth during shutdowns lasting longer than 24 hours,
flush the pump and solvent lines with a solution of organic solvent/water in
which the organic component is greater than 20%.
Caution:
introducing incompatible buffers, consult the instrument's
accompanying documentation to determine what buffers are compatible.
To avoid salt precipitation, nonvolatile buffer concentrations must not
Tip:
exceed 100 mM.
Solvent recommendations
This section lists solvents recommended for the ACQUITY UPLC M-Class
System. Contact Waters Customer Service to determine whether you can use
solvents that do not appear in the list without adversely affecting performance
of system modules or the system itself.
Recommended solvents
• Acetonitrile
• Acetonitrile/water mixtures
• Isopropanol
• Methanol
• Methanol/water mixtures
• Water
Solvents to avoid
Do not use these solvents:
• Solvents that contain halogens: chlorine, fluorine, bromine, or iodine.
• Strong acids. (Use them only in weak concentration, less than 5%,
unless using them as cleaning agents. Avoid using acids as mobile
phases when their pH is less than 2.0.)
To avoid damaging a mass spectrometer by unwittingly
Solvent recommendations
March 10, 2014, 715003588 Rev. A 99