Waters ACQUITY UPLC M-Class System Manual page 100

D Solvent Considerations
• Solutions that contain strong concentrations (greater than 0.1% wt.) of
complexing agents like ethylenediaminetetraacetic acid (EDTA).
• Compounds that form peroxides, such as UV-grade ethers, dioxane, and
diisopropylether.
Recommended cleaning agents
You may use these cleaning agents:
• Formic acid (≤10%)
• Phosphoric acid (≤30%)
• Sodium hydroxide (≤1Μ)
System recommendations
Caution:
system filters and capillary lines, add a small amount (less than 10%) of
an organic solvent, such as acetonitrile or methanol, and completely
replace the mobile phase daily. Aqueous solvents must not remain in a
shut-down system because they serve as a substrate for microbial
growth.
• Contact Waters for recommended system cleaning and flushing
procedures.
• Contact your Waters sales representative or local technical support
organization to determine the typical chromatography conditions or
operating parameters suitable for ACQUITY UPLC M-Class system
modules.
• Chloroform, methylene chloride, halogenated solvents, and toluene can
be used in weak dilutions (less than 10% vol.) as additives, modifiers, or
sample diluents.
• Methanesulfonic acid is not recommended for use in ACQUITY UPLC
M-Class systems.
• Always use properly cleaned and maintained glassware.
100 March 10, 2014, 715003588 Rev. A
To prevent the proliferation of microbial colonies that clog