Waters ACQUITY UPLC M-Class System Manual page 58

4 Maintaining the system
ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
No peaks
(continued)
All peaks affected
Fronting peaks
58 March 10, 2014, 715003588 Rev. A
Suspected cause
No spray
Incorrect vial position
Injector not injecting
Incorrect detector settings
Injection volume/sample
concentration too high
disrupting column
equilibration
Injection volume or
sample concentration is
too high disrupting
column equilibrium
Sample diluent is too high
in organic for the initial
mobile phase
Needle weak wash solvent
is too high in organic
Possible solution
The Universal
See also:
NanoFlow Sprayer
Installation and
Maintenance Guide on the
documentation CD.
Ensure vial is in correct
position.
• Prime the sample
syringe.
• Consult the console
online Help.
The applicable
See also:
detector overview and
maintenance guide on the
documentation CD and
the online Help.
• Reduce the injection
volume.
• Dilute the sample.
Reduce the injection
volume or dilute the
sample.
Reduce the injection
volume or reduce the
percentage of organic.
Ensure that the weak
needle wash solution is
similar to the initial
mobile phase.
The volume of weak
Note:
needle wash should be
three-times strong wash
volume.