Waters ACQUITY UPLC M-Class System Manual page 57

ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Early eluting peaks
fail
Late-eluting peaks
fail
No peaks
Suspected cause
Injection volume or
sample concentration is
too high
Sample diluent too high in
organic for the initial
mobile phase
Needle weak wash solvent
is too high in organic
The method or part of the
method is isocratic
Incorrect trapping or
column conditions
Inappropriate gradient
method
Incorrect trapping
conditions
Incorrect sample
preparation
No solvent flow
Leak in the solvent path
March 10, 2014, 715003588 Rev. A
Troubleshooting
Possible solution
• Reduce the injection
volume.
• Dilute the sample.
Reduce the injection
volume or reduce the
percentage of organic in
the diluent.
Ensure that the needle
wash solution is similar to
the initial mobile phase.
Use a gradient method in
which all peaks are
typically the same width.
Use standard trapping
conditions and condition
the column properly.
Use the correct gradient
method.
Use the standard trapping
conditions.
Prepare the sample
correctly.
• Check that there is
sufficient solvent in the
bottles.
• Ensure the solvent flow
rate is >0.0 and the
system pressure is
normal.
Inspect all fittings and
replace as necessary.
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