Waters ACQUITY UPLC M-Class System Manual page 98

D Solvent Considerations
Dirty solvents can cause baseline noise and drift, and they can clog solvent
reservoir filters, inlet filters, and capillary lines.
Solvent quality
Use LC/MS-grade solvents for the best possible results. Filter solvents
through a 0.2-μm membrane filter. Small particles can permanently block a
system's capillary lines. Filtering solvents also improves check-valve
performance.
Recommendation:
that are compatible with each other and that you follow the manufacturers'
recommendations.
Solvent preparation
Proper solvent preparation, primarily filtration, can prevent many pumping
problems.
Store mobile phases in borosilicate glass reservoirs type 1, class A
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3.3 . Use brown-stained glassware to inhibit microbial growth. Use aluminum
foil or Waters caps to cover the reservoirs.
Water
Use water only from a high-quality water purification system. If the water
system does not deliver filtered water, filter the water through a 0.2-μm
membrane filter.
Recommendation:
Caution:
system filters and capillary lines, add a small amount (less than 10%) of
an organic solvent, such as acetonitrile or methanol, and completely
replace the mobile phase daily. Pure aqueous solvents must not remain
in a shut-down system because they serve as a substrate for microbial
growth.
Using buffers
Adjust the pH of aqueous buffers. Filter them to remove insoluble material,
and then blend them with appropriate organic modifiers. After you finish
98 March 10, 2014, 715003588 Rev. A
Ensure that you choose solvents and membrane filters
Use LC/MS-quality (or better) water.
To prevent the proliferation of microbial colonies that clog
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or type