Waters ACQUITY UPLC M-Class System Manual page 64

4 Maintaining the system
ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Loss of sensitivity
(continued)
64 March 10, 2014, 715003588 Rev. A
Suspected cause
μSM-FL temperature is
too high or low
Sample syringe draw
speed set too high,
resulting in too little
sample draw-up
Sample diluent
composition contains too
much organic resulting in
poor peak shape
Vial position is incorrect
Incorrect sample
preparation and sample
composition resulting in,
or due to, one or more of
the following:
• Sample concentration
too low
• Sample is not
completely in solution
• Volatile sample is
evaporating
• Sample is degraded
• Sample precipitated
over time
• Sample prepared
incorrectly
• Sample buffers and
acids inappropriate for
the injection method
selected
Possible solution
Adjust the μSM-FL
sample compartment
temperature.
From the Method Editor,
program a slower draw
speed.
Decrease organic in the
sample diluent.
Program the correct vial
position in the sample list.
Select the appropriate
sample preparation
method for the injection
method selected and
remake if necessary.