Acquity Uplc M-Class System - Waters ACQUITY UPLC M-Class System Manual

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ACQUITY UPLC M-Class system

®
Waters designed the ACQUITY UPLC M-Class system to perform a range of
chromatographic separations from nano-scale to micro-scale. With a
maximum operating pressure of 103,421 kPa (1034 bar, 15,000 psi), an
analytical flow rate to 100 μL/min, and a trapping flow rate to 1 mL/min, the
system produces optimal linear velocity for columns that range in internal
diameter from 75 μm to 1.0 mm.
The system is compatible with Waters mass spectrometers and optical
detectors, supporting a range of column diameters. Options include:
• NanoFlow™ ESI and NanoLockSpray™ sources for Waters
time-of-flight and tandem MS, respectively, for flow rate ranges from
200 nL/min to 2,000 nL/min.
• micro-scale ESI probe for use in the ZSpray™ API and LockSpray dual
electrospray ion sources for flow rates ranges from 5 μL/min and
100 μL/min.
• A 100-nL, light-guided, flow cell for columns ranging from 300 μm to
1.0 mm I.D with the ACQUITY UPLC PDA and TUV detectors. A 10-nL,
transverse-illuminated, flow cell for nano-scale separations with the
ACQUITY UPLC TUV detector.
ACQUITY UPLC M-Class systems that include mass spectrometry, optical
detection, or both can analyze compounds found in low-concentrations amid
complex matrices, quantifying peptides or metabolites (when used with a
tandem mass spectrometer) or performing other, targeted experiments.
Contents:
Topic
Configurations of system modules .................................................. 16
System components.......................................................................... 18
Additional information .................................................................... 23
March 10, 2014, 715003588 Rev. A
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