Waters ACQUITY UPLC M-Class System Manual page 55

ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Increasing or
decreasing
retention times
(continued)
Suspected cause
Incorrect, degraded or
contaminated column
The system is not
stabilized or chemically
equilibrated
March 10, 2014, 715003588 Rev. A
Troubleshooting
Possible solution
• If contaminated, flush
the column with 85%
organic solvent.
• Otherwise, clean or
replace the column as
described in the
appropriate columns
care and use manual on
the documentation CD.
• Allow all system
components (such as
the column and
detector) sufficient time
to stabilize and
chemically equilibrate.
• Ensure proper
operating conditions for
the application (such as
mobile phase, detector
settings and detector
type).
The component's
See also:
overview and maintenance
guide or the column care
and use manual, on the
documentation CD, for
recommended
equilibration times.
When running an
Note:
automated gradient
method, ensure that there
is sufficient and
reproducible equilibration
times between injections.
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