Waters ACQUITY UPLC M-Class System Manual page 61

ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Split or double
peaks
Suspected cause
Injection volume or
sample concentration is
too high disrupting
column equilibration
Sample diluent is too high
in organic for the initial
mobile phase
Needle weak wash solvent
is too high in organic
In-line filter, column inlet
or connecting tubing is
partially blocked
Column(s) contaminated
Column degraded forming
a void
Trap column degraded
March 10, 2014, 715003588 Rev. A
Troubleshooting
Possible solution
• Reduce the injection
volume.
• Dilute the sample.
Reduce the injection
volume or reduce the
percentage of organic in
the diluent.
Ensure that the weak
needle wash solution is
similar to the initial
mobile phase.
Inspect these components
for particle build-up.
Clean or replace.
Replace column(s).
The appropriate
See also:
column care and use guide
on the documentation CD.
Verify column
performance. If efficiency
is low, replace it.
• Remove trap column
and perform a direct
injection.
• If results are normal,
replace the column and
perform a set pressure
diagnostic.
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