Waters ACQUITY UPLC M-Class System Manual page 103

Properties of common solvents: (Continued)
Solvent
Triethylamine
Trifluoroacetic acid
Water
o-xylene
Solvent miscibility
Before you change solvents, refer to the table below to determine solvent
miscibility. Be aware of these effects:
• Changes involving two miscible solvents can be made directly. Changes
involving two solvents that are not totally miscible (for example, from
chloroform to water) require an intermediate solvent like n-propanol.
• When you switch from a strong buffer to an organic solvent, thoroughly
flush the system using water before you add the organic solvent (see
"Water" on page
• Temperature affects solvent miscibility. If you are running a
high-temperature application, consider the effect of the higher
temperature on solvent solubility.
• Buffers dissolved in water can precipitate when mixed with organic
solvents.
Solvent miscibility:
Polarity
Solvent
index
0.0 N-hexane
1.8 Triethylamine
4.3 1-propanol
4.3 2-propanol
5.2 Ethanol
5.5 Benzyl alcohol
Vapor Pressure
mm Hg (Torr)
57 at 25 °C
97.5 at 20 °C
17.54 at 20 °C
6 at 20 °C
98).
Viscosity
cP, 20 °C
(@1 atm)
0.313
0.38
2.30
2.35
1.20
5.80
March 10, 2014, 715003588 Rev. A
Boiling
Point (°C)
89.5
71.8
100.0
144.41
Boiling Miscibility
point °C number
(@1 atm) (M)
68.7 29
89.5 26
97.2 15
117.7 15
78.3 14
205.5 13
Solvent miscibility
Flash
Point (°C)
-9
-3
17
λ Cutoff
(nm)
––
––
210
––
210
––
103