Qsm And Bioqsm Performance Specifications - Waters ACQUITY UPLC Overview And Maintenance Manual

B.5 QSM and bioQSM performance specifications
Attribute
Number of solvents One to four (A, B, C, and D), in any combination. Optional 6-position
Solvent degassing
Gradient formation
Gradient profiles
Primary check
valve
Flow accuracy
Flow precision
Composition ripple
(baseline noise)
Specification
solvent selection valve enables solvent selections D1 through D6 on line
D, in addition to A, B, and C (a total of nine solvents to select from).
Integrated vacuum degassing, four chambers. One additional chamber for
the sample manager purge solvent.
Low-pressure mixing, quaternary gradient
11 gradient curves, including linear, step (2), concave (4), and convex (4)
Intelligent Intake Valve (i
Passive check valve, optional
±1.0% of set flow at 0.500 to 2.000mL/min using 100% solvent A (with
2
i Valve).
Back pressure 4137 to 6895 kPa (41 to 69 bar, 600 to 1000 psi), with
degassed water.
0.075% RSD or ±0.020 min SD, whichever is greater, based on six
2
replicates (i Valve).
Test conditions:
• Mobile phase: 60:40 water/methanol mixed via Auto•Blend Plus
technology
• Flow rate: 0.5 mL/min
• Sample mix: alkylphenone mix (5.0 µL injection volume)
• Column: ACQUITY UPLC BEH C
• Column temperature: 35 °C ±0.3 °C
• Detector: UV, 254 nm wavelength
<1.0 mAU (<0.1 mAU with optional 250-µL mixer), with i
Test conditions:
• Mobile phase: A: water + 0.1%, trifluoroacetic acid; B: acetonitrile +
0.1% trifluoroacetic acid
• Flow rate: 0.5 mL/min
• Gradient conditions: 1.0 to 33% B in 10 min; time average window, 10
s. Noise range 4.00 to 6.00 min
• Column: ACQUITY UPLC BEH C
• Detector: ACQUITY TUV, 214 nm wavelength, 10 Hz sampling rate
August 8, 2016, 715005050 Rev. C
Page 139
2
Valve), standard
, 1.7 µm, 2.1 × 50 mm
18
, 1.7 µm, 2.1 × 50 mm
18
2
Valve