Waters ACQUITY UPLC M-Class System Manual page 62

4 Maintaining the system
ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Tailing peaks with
a normal retention
time
Tailing peaks with
increased retention
time
Loss of sensitivity
62 March 10, 2014, 715003588 Rev. A
Suspected cause
Fitting or interconnection
problem
Bad consumables
Leak in the system
Void volume or bad
consumables
Wrong injection volume;
less sample loaded on a
column will result in a
lower response
Needle wash solvent
composition; weak wash
too high in organic solvent
The programmed gradient
table is incorrect
Possible solution
Ensure proper sprayer or
emitter plumbing.
The Universal
See also:
NanoFlow Sprayer
Installation and
Maintenance Guide on the
documentation CD.
Perform a systematic
replacement of
consumables beginning
with the analytical
column.
• Autozero the flow
transducers.
• If the problem persists,
perform the set
pressure diagnostic test
to check for system
leaks.
The online Help
See also:
for more information.
Perform a systematic
replacement of
consumables beginning
with the analytical
column.
Change to correct injection
volume.
Make fresh weak wash
solvent.
Correct the gradient table.