Waters ACQUITY UPLC M-Class System Manual page 63

ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Loss of sensitivity
(continued)
Suspected cause
The programmed flow rate
is too low
Incorrect mobile phase
due to one or more of the
following:
• Percentage of organic
use is incorrect
• Wrong solvent used
• Programming error
• Wrong pH
• Mobile phase
contaminated
Column temperature is
programmed too low
Needle depth is
programmed too high or
too low
Incorrect injection method
selected in the method
setup.
Needle and sample loop
volume are not calibrated
or have been configured
incorrectly
Plate configuration is
incorrect, so needle does
not reach the sample
March 10, 2014, 715003588 Rev. A
Troubleshooting
Possible solution
Program the correct flow
rate ensuring it is
appropriate for the column
I.D.
Make fresh mobile phase
and ensure that the
solvent used and gradient
programmed is
appropriate for the
method.
Set the correct column
temperature.
• Verify needle depth
setting.
• Ensure correct sample
volume.
• Ensure correct vial or
wellplate type.
Check, and if necessary,
adjust the injection
method configuration in
the Console.
Verify the needle and
sample loop
characterization, if
necessary, re-characterize
the volumes.
Set the correct plate
configuration.
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