Waters ACQUITY UPLC M-Class System Manual page 53

ACQUITY UPLC M-Class System troubleshooting: (Continued)
Symptom
Erratic retention
times
Suspected cause
The system is not
stabilized or chemically
equilibrated
Erratic solvent manager
pressure
Injection volume or
sample concentration is
too high, disrupting
equilibrium
March 10, 2014, 715003588 Rev. A
Troubleshooting
Possible solution
• Allow all system
components (such as
the column and
detector) sufficient time
to stabilize and
chemically equilibrate.
• Ensure proper
operating conditions for
the application (such as
mobile phase, detector
settings and detector
type).
The component's
See also:
overview and maintenance
guide or the column care
and use manual, on the
documentation CD, for
recommended
equilibration times.
When running an
Note:
automated gradient
method, ensure that there
is sufficient and
reproducible equilibration
times used between
injections.
Analyze the pressure trace
in the Console.
Reduce the injection
volume or dilute the
sample with initial mobile
phase.
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