Table of Contents
Related Manuals for Olympus 1X71
Summary of Contents for Olympus 1X71
- Page 1 UCL Institute of Child Health User guide Olympus 1X71 Dr Bertrand Vernay Light Microscopy Facility Manager Wellcome building, Office W2.09 Tel Office: 42224 Tel Mobile: 07582249337 Email: b.vernay@ucl.ac.uk Revised December 2014...
- Page 2 Table of contents page 3 ..Ownership page 3 ..Access Rules page 3 ..Olympus Customer Support Contact page 4 ..General Specifications Microscopy Techniques Available Objectives Filter Cubes Camera Fluorescence Illumination Pixels to Microns Calibration Consumables List page 5 ..Quick User Guides...
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Page 3: Ownership
Users must always record their activity in the Log book Problem(s) with the microscope should be reported as soon as they are noticed Olympus customer support service: http://www.olympus.co.uk/microscopy The system is not covered by a maintenance contract Olympus requires a PO number before sending an engineer... -
Page 4: General Specifications
Olympus LUCPlanFLN 20x Ph1 NA 0.45 WD 6.6 -7.8 with correction collar Olympus LUCP lanFLN 40x Ph2 NA 0.6 WD 3.0 - 4.2 with co rrection collar Z e i s s o b j e c t i v e s c a n a l s o b e u s e d... -
Page 5: Quick User Guides
Quick user guides Users must always record their activity in the Log book Transmitted light 1 . H al oge n L amp Po w e r S u pply U n i t TH 4 "O N " 2 . L i gh t Path s e le ctor on "O cu lar" 3 . -
Page 6: Transmitted Light
Halogen lamp operation: Turning on the lamp 1. Make sure the light intensity control knob (5) is in the MIN (minimum intensity) position on the microscope frame. 2. Make sure the light intensity control knob (1) is in the MIN (minimum intensity) position on the TH4 module. -
Page 7: Halogen Lamp Operation
Halogen lamp operation: Turning off the lamp 1. Set the light intensity control knob (5) to the MIN (minimum intensity) position on the microscope frame. 2. Set the light intensity control knob (1) to the MIN (minimum intensity) position on the TH4 module. -
Page 8: Kohler Illumination
Kohler illumination: 1. Rotate the turret (1) to the "BF" position. (Any of positions 3,4 or 5, position 1=Ph1, 2 = Ph2) 2. Sl i d e t h e ap e r tu re ir i s d i ap hr ag m le v er ( 2 ) t o fu l l y o p en th e d i aph r ag m . 3. -
Page 9: Adjusting The Objective Correction Collar
Adjusting the objective correction collar Correction is possible according to the vessel bottom thickness. 1. When the thickness of the vessel bottom is known, match the scale reading of the correction collar to the thickness of the vessel in use. 2. -
Page 10: Prior Lumen200 Metal Halide Lamp Operation
Prior Lumen200 Metal Halide Lamp Operation: Starting Up the Lumen 1. Switch the Lumen power switch on. 2. Make sure the ventilation vent on the left hand side is unobstructed or the lamp will overheat resulting in automatic shutdown and damage to the module. 3. -
Page 11: Image Capture With Hclmage
Image Capture with HClmage 1. Click the Capture pane. 2. Click Live for a live image from the camera 3. Camera binning or image sub-array can be set in the Binning SubArray panel. 4. In the Camera Control panel, adjust exposure/gain manually or automatically by clicking on Auto Expose;... -
Page 12: Selecting The Right Fluorochrome/Filter Set
Selecting the right fluorochrome/filter set Position #1 49000 - ET - DAPI Exciter 350/50x beamsplitter 40011 Emitter ET460/50m Wavelength (nm) AT350/50x T4001p ET460/50m Fluorochrome EM Use Alexa Fluor 350TM 346 442 Recommended Coumarin 384 470 Recommended DAPI 359 461 Recommended DyLight 350 352 435 Recommended Hoechst 33258... -
Page 13: Endow Gfp/Egfp Bandpass
Position #2 41017 EndowGFP/EGFP Bandpass Emitter ET470/40x Beamsplitter 495LP Emitter ET 525/50m Wavelength (nm) H0470/40x Q4951p Fluorochrome HQ525/50m Acridine Orange + DNA Alternative Alexa Fluor 488T" Alternative Azami Green Alternative BODIPY FL/pH7.2 Alternative Calcein Alternative Calcium GreenTM-1 Alternative Cy2TM Alternative Alternative... -
Page 14: Dsred(Tritc/Cy3
Position #3 49005 - ET - DSRed (TRITC/Cy3) Exciter ET545/30x Beamsplitter T570LPXR Emitter ET620/60m I — 0 ____________________ Wavelength (nm) -- ET545/30x --- T5701p -- ET620/60m Fluorochrome AsRed2 Recommended Ethidium Bromide Recommended Ethidium homidimer-1/DNA Recommended Propidium Iodide Recommended Resorufin Recommended Alexa Fluor 568TM Alternative Cy3TM... - Page 15 Position #4 49006 - ET - Cy5 emitter ET620/60x Beamsplitter T660LPXR Emitter ET700/75n, Fluorochrome Alexa Fluor 647TM Recommended Allophycocyanin (APC) Recommended Atto 647N Recommended Cy5TM Recommended Recommended Draq5 Recommended DyLight 649 Recommended MitoTracker Deep Red 633/MeOH Recommended Nile Blue Recommended SYTO®...
- Page 16 Position #5 49007 - ET - Cy7 Exciter ET710/75x Beamsplitter T760LPXR Emitter ET810/90m I — ; s t ______ 600 650 Wavelength (nm) -- ET710/75x -- — T7601pxr -- ET810/90m Fluorochrome Alexa Fluor 750TM Recommended Cy7TM Recommended Recommended DyLight 750 Recommended...
- Page 17 HClmage Live...