Reflected Light Nomarski Differential Interference Contrast (Dic) Observation - Olympus BX-URA2 Instruction Manual

Reflected fluorescence system
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4-2

Reflected Light Nomarski Differential Interference Contrast (DIC) Observation

# The performance of polarizer may deteriorate when it has been exposed to light for a long period (about
continuous 2000 hours). If this happens, replace the polarizer.
# When using the high-intensity light source, be sure to use the U-25L42 filter for prevention of the polarizer burn.
}When performing sensitive color observation using the U-DICRH DIC slider, combine the U-POTP3 polarizer.
@
Fig. 24
@
40
²
|
³
Fig. 25
1
Selecting the Light Path for Observation
1. Rotate the turret to engage the BF mirror unit @ in the light path.
Reflected light Nomarski
DIC
}When the U-MDIC3 DIC mirror unit is mounted in the turret, engage the
DIC mirror unit in the light path. The analyzer and polarizer are set to the
"Crossed Nicol" position so adjustment is not required.
2. Engage the U-AN360-3 analyzer and U-PO3 polarizer in the light path.
3 Rotate the analyzer dial until complete extinction (crossed Nicol position)
is obtained.
2
Installing the Nomarski Prism
1. Loosen the DIC clamping knob @ at the front of the DIC revolving nose-
piece, and insert the DIC prism ² with the inscription facing upward.
2. With the U-DICR interference slider, set the slide lever ³ according to the
objective in use.
Lever ³ position
UIS2
Pushed in
UIS
UIS2
Pulled out
UIS
Inscription Mirror Unit
BF
U-MBF3
DIC
U-MDIC3
Analyzer/polarizer built in
Applicable Objectives
MPLFLN/MPLFLN-BD series
UMPlanFl/UMPlanFl-BD series
MPlanApo20X, 100X
MPlanApo100XBD
LMPLFLN/LMPLFLN-BD series
LMPlanFl/LMPlanFl-BD series
LMPlanApo/LMPlanApo-BD series
(Fig. 24)
Note
(Fig. 25)

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