Olympus CK30 Instructions Manual
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Olympus CK30 Instructions Manual

Culture microscope
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INSTRUCTIONS
CK30/CK40
CULTURE MICROSCOPE
This instruction manual is for the Olympus Culture Microscope Model CK30/CK40. To ensure the
safety, obtain optimum performance, and to familiarize yourself fully with the use of this microscope,
we recommend that you study this manual thoroughly before operating the microscope. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 1 6 2

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Summary of Contents for Olympus CK30

  • Page 1 CK30/CK40 CULTURE MICROSCOPE This instruction manual is for the Olympus Culture Microscope Model CK30/CK40. To ensure the safety, obtain optimum performance, and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating the microscope. Retain this instruction manual in an easily accessible place near the work desk for future reference.

  • Page 2: Safety Precautions

    The CK30 and CK40 Culture Microscopes have different system configurations. The differences are shown in the table below. CK30 CK40 Observation tube Built-in binocular tube Interchangeable* Stage plate – Interchangeable** CK40-RFL reflected light Not mountable Mountable fluorescence attachment * The CH3-BI45 binocular tube, CH3-TR45 trinocular tube, and CK40-TBI Tilting binocular tube are all mountable.
  • Page 3 Safety Symbols The following symbols are found on the microscope. Study the meaning of the symbols, and always use the equipment in the safest possible manner. Symbol Explanation Indicates that the surface becomes hot, and should not be touched with bare hands. Before use, carefully read the instruction manual.

  • Page 4: Maintenance And Storage

    Maintenance and Storage 1. Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and alcohol (30%). # Do not use a mixture of ether (70%) and alcohol (30%) to clean the lower lens (made of optical plastic) of the eyepiece (NCWHK10X) because such solvents cloud the lens.

  • Page 5: Table Of Contents

    CK30/CK40 NOMENCLATURE ASSEMBLY Assembly Diagram ................................... 3 Detailed Assembly Procedure ..........................4 CONTROLS SUMMARY OF OBSERVATION PROCEDURES USING THE CONTROLS Microscope Body ..................................12 Stage ........................................... 13 Observation Tube ..................................14 Illumination Unit ..................................17 Objectives ......................................18 PHASE CONTRAST OBSERVATION...

  • Page 6: Nomenclature

    NOMENCLATURE CK30 Lamp socket U-LS30-3 Objective For observation: EDAch4X EDAch10X LWDCDAch20X # A dedicated objective is required for ordinary phase contrast obser- vation and relief phase Illumination unit contrast observation. support (See page 19.) (stationary) Eyepiece · NCWHK10X · WHK10X ·...
  • Page 7 CK30/CK40 CK40 Lamp socket U-LS30-3 Objective For observation: EDAch4X EDAch10X LWDCDAch20X # A dedicated objective is required for ordinary phase contrast obser- vation and relief phase Illumination unit contrast observation. support (stationary) Phase contrast Eyepiece slider · NCWHK10X · Ph precentering slider: ·...

  • Page 8: Assembly

    · CK30-F in the same position as the · CK40-F stage extension plate. Mechanical stage ** The CK30 is provided with sta- CK40-MVR tionary binocular tube. ***The CK30 is not provided with Stage extension plate CK2-SS...

  • Page 9: Detailed Assembly Procedure

    CK30/CK40 2-2 Detailed Assembly Procedure Mounting and Replacing the Bulb (Fig. 3) }Use only the specified Philips 5761 halogen bulb, 6 V 30 W HAL . ² To prevent reduced bulb life or cracking, do not touch the bulb with bare hands.
  • Page 10 Mounting the Objectives (Fig. 6 & 7) # First raise the revolving nosepiece slightly to remove the transporta- tion pad on the nosepiece’s base. }Keep the transportation pad in a safe place. You will need it when the equipment is sent for repair or transported to another location. 1.
  • Page 11 CK30/CK40 Mounting the Stage Plate (CK40 Only) (Fig. 9) ² Fit the standard stage plate @ into the opening on the stage. # Turn the standard stage plate so that the notch ² faces to the front for easy confirmation of an objective tip. When using the glass stage plate, set it in the direction so that its product code inscription “...
  • Page 12 Mounting the Eyepieces (Fig. 12) Insert the eyepiece @ into the eyepiece sleeve ² on the observation tube. ² Fig. 12 Mounting the Filter (Fig. 13 & 14) ² Let the filter cool down sufficiently before replacing the filter. Remove the filter holder @ and mount the required filter ². # Push the filter down to the bottom as shown in Fig.
  • Page 13 Do not connect anything other than the lamp cord plug @ to the Fig. 15 connector ². Doing so will cause equipment failure. Always use the power cord provided by Olympus. If no power cord is provided, please select the proper power cord by referring to the ‡...

  • Page 14: Controls

    CONTROLS CK30 Phase contrast slider Filter holder Diopter adjustment ring Aperture iris diaphragm lever Interpupillary distance scale Brightness adjustment control Slider centering lever storage holes Fine adjustment knob Coarse adjustment knob Coarse tension adjustment ring Main switch...
  • Page 15 CK30/CK40 CK40 Filter holder Phase contrast slider Aperture iris diaphragm lever Knurled sliding grips Diopter adjustment ring Standard stage plate Interpupillary distance scale Brightness adjustment control Slider centering lever storage holes Fine adjustment knob Coarse adjustment knob Coarse tension adjustment ring...

  • Page 16: Summary Of Observation Procedures

    SUMMARY OF OBSERVATION PROCEDURES 1. Set the main switch @ to “ I ” (ON) and turn the brightness adjustment control ² to obtain appropriate light intensity. (Page 12) ² 2. When using the CK40, push in the light path selector ³ on the trinocular tube to set the light path at 100% for binocular observation.

  • Page 17: Using The Controls

    CK30/CK40 USING THE CONTROLS 5-1 Microscope Body Turning on the Light Source (Fig. 17) Set the main switch @ on the side panel of the microscope body to “ I ” (ON). Fig. 17 Adjusting the Brightness Adjustment Control (Fig. 18) Turn the brightness adjustment control @ clockwise to raise the voltage and increase the light intensity.

  • Page 18: Stage

    }When the standard stage plate is mounted on the CK40, the 35 mm petri dish can be mounted directly on the stage. 1. When using the CK30, put the provided 35 mm petri dish holder @ on the Fig. 20 stage and mount the 35 mm petri dish on the opening in the center.

  • Page 19: Observation Tube

    Fig. 23 When using the CK40 þ Binocular tube and tilting binocular tube ý Follow the procedure in “ When using the CK30 ”. þ Trinocular tube ý ² 1. While looking through the eyepieces, slide the knurled sliding grips @ until the left and right fields of view fully coincide.
  • Page 20 Adjusting the Diopter (Figs. 25 & 26) When using the CK30 1. While looking through the left eyepiece with your left eye, turn the coarse and fine adjustment knobs to bring the specimen into focus. 2. While looking through the right eyepiece with your right eye, turn only the diopter adjustment ring @ to focus on the specimen.
  • Page 21 CK30/CK40 Adjusting the Tilt (CK40-TBI Only) (Fig. 28) 30°– 60° }The height and angle of the binocular tube can be adjusted to any position, giving you more freedom to find a comfortable observation posture. Hold the binocular assembly with both hands and move it up or down until the tube is tilted in a way that you find comfortable and facilitates easy observation.

  • Page 22: Illumination Unit

    5-4 Illumination Unit Using the Filter }Use filters as needed to increase the accuracy of observation and photo- micrography. The LBD filter is especially recommended for observation and photomicrography since it achieves more neutral colors. }Filters can be stacked in the filter holder. (Maximum thickness: 11 mm) Filter Purpose IF550...

  • Page 23: Objectives

    CK30/CK40 5-5 Objectives Setting the Correction Collar (Fig. 32) A culture microscope is designed to observe specimens contained in vessels of various bottom thicknesses. In order to achieve optimum objective performance of the culture microscope, the high N . A . objec- tives are provided with a correction collar @.

  • Page 24: Phase Contrast Observation

    PHASE CONTRAST OBSERVATION The following three units are available for phase contrast observation. Mount a slider onto the microscope and replace the objectives with phase-contrast-compatible objectives. Model name Description Compatible objectives · The light annuli are precentered, so no adjust- Ph precentering slider SPlan4XPL-6 ment is required.
  • Page 25 CK30/CK40 Names of Parts Phase Slider Ph precentering slider CK40-SLP # Mountable 40X objective: LWDCDPlan 40XFPL-6 only. For 4X Empty For 10X, 20X & 40X Ph centering slider CK40-SL # Mountable 40X objective: LWDCDPlan 40XPL-6 only. Light annulus for the...
  • Page 26 Mounting the Light Annulus for the (Fig. 33) 40X Objective }When observing with the 40X objective, you must use a light annulus exclusively designed for the phase contrast slider you are using. The CK2-RS40 must be mounted on the CK40-SL and the CK40-RPS40 on the CK40-RPSL.
  • Page 27 CK30/CK40 Centering the Light Annulus or (Figs. 35 & 36) Relief Slit # The CK40-SLP does not need to be centered. ² 1. Place a specimen on the stage and bring it into focus. 2. Replace the objective in the sleeve which does not have a diopter ad- justment ring with the CT-5 centering telescope.

  • Page 28: Photomicrography

    PHOTOMICROGRAPHY 7-1 Connecting to the Phtomicrographic Systems OM camera back C-mount Video camera Automatic photomicrographic system PM-30 Photomicro adapter L Photomicrographic Video adapter MOM-L system Automatic photomicrographic system MTV-3 PM-10M PM20 Automatic exposure photomicrographic system PM-10AK Photo eyepiece NFK NFK photo eyepiece adapter PM-ADF Trinocular tube CH3-TR45...
  • Page 29 CK30/CK40 Mounting the Camera Unit (Fig. 37) Camera Unit 1. Loosen the clamping screw ² of the PM-ADF NFK photo eyepiece adapter @. 2. Attach the adapter @ to the CH3-TR45 trinocular tube ³ and tighten the ² screw ².

  • Page 30: Specifications

    SPECIFICATIONS Specifications Item CK30 CK40 1. Optical system LB optical system (finite-corrected system) 2. Illumination 6 V 30 W HAL high-intensity halogen bulb Philips 5761 (Average service life: Approx. 100 hrs. when used as directed) Output rating: 6 V 30 VA Input rating: 100V area –...
  • Page 31 Stage dimensions: 160 (W) X 250 (D) mm Stage extension plate dimensions: 70 (W) X 180 (D) mm Provided with ø35 mm petri dish holder (CK30 only) 9. Mechanical stage Traversing area: 120 (X) X 78 (Y) mm; coaxial low drive control knobs on right or left side of plain stage;...

  • Page 32: Troubleshooting Guide

    Under certain conditions, performance of this unit may be adversely affected by factors other than defects. If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact your local Olympus representative for assistance. Trouble...
  • Page 33 CK30/CK40 Trouble Cause Remedy Page e) Resolution problems The light annulus and phase annulus are When using the CK40-SL or CK40-RPSL · Images are not sharp. not centered. center them correctly. · Contrast is wrong. The objective is not compatible with Use one compatible with phase contrast ·...
  • Page 34 Trouble Cause Remedy Page 5. Photomicrography a) The picture is out of focus. You are using the OM camera with the Use the ND filter and select a slower shut- shutter speed set faster than 1/2 sec. ter speed. b) The edges are blurred. You are using an achromatic objective.

  • Page 35: Proper Selection Of The Power Supply Cord

    If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “ Specifications ” and “ Certified Cord ” below: CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 36 Table 2 HAR Flexible Cord APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS Alternative Marking Utilizing Printed or Embossed Harmoniza- Black-Red-Yellow Thread (Length tion Marking (May be located on Approval Organization of color section in mm) jacket or insulation of internal wir- ing) Black Yellow...
  • Page 37 MEMO...
  • Page 38 MEMO...
  • Page 39 2-43-2,Hatagaya, Shibuya-ku, Tokyo, Japan Postfach 10 49 08, 20034, Hamburg, Germany 2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 2-8 Honduras Street, London EC1Y OTX, United Kingdom. 104 Ferntree Gully Road, Oakleigh, Victoria, 3166, Australia This publication is printed on recycled paper.

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