Reflected Light Differential Interference Contrast (Dic) Observation; Procedures - Olympus BX53M Instructions Manual

4-3
Reflected light differential interference contrast (DIC) observation procedures
1
Turn ON the main switch.
2
Select the reflected light illumination
3 Select the differential interference contrast
/ polarization (DIC/POL) observation.
4 Set the analyzer and the polarizer.
5
6 Select the light path. (Trinocular tube only)
7 Place a sample on the stage.
8 Engage the 10X objective in the light path.
9 Bring the sample into focus.
13 Adjust the aperture diaphragm and the field diaphragm.
14 Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
16
Adjust the prism of the DIC slider.
54
Set the DIC slider.
10 Adjust the brightness.
11
Adjust the interpupillary distance.
12
Adjust the diopter.
15
Adjust the brightness.
Start observation.
Main switch
Transmitted/reflected light switch
Observation method selector knob of the
reflected light illuminator
Analyzer insertion slot / polarizer insertion slot
of the reflected light illuminator
Slider insertion slot of the nosepiece (page 62)
Light path selection lever of the trinocular
tube
Stage plate
X-axis / Y-axis knob of the stage
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
Binocular tube
Diopter adjustment ring
Aperture diaphragm knob of the reflected
light illuminator
Field diaphragm knob of the reflected light
illuminator
Nosepiece
Coarse / Fine focusing knob of the
microscope frame
Brightness control knob of the microscope
frame
Prism movement knob of the DIC slider (page
63)
(page 16)
(page 16)
(page 17)
(page 60)
(page 19)
(page 19)
(page 19)
(page 23)
(page 24)
(page 26)
(page 32)
(page 32)
(page 39)
(page 36)
(page 23)
(page 24)
(page 26)