Olympus CX33 Instructions Manual
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Olympus CX33 Instructions Manual

Biological microscope
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INSTRUCTIONS
CX33
Biological Microscope
This instruction manual is for the Olympus biological microscope.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating this
microscope, and always keep this manual at hand when operating this product.
Optical Microscope and Accessory
Retain this instruction manual in an easily accessible place near the work desk for future
reference.
AX 8881

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Summary of Contents for Olympus CX33

  • Page 1 CX33 Biological Microscope This instruction manual is for the Olympus biological microscope. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of this microscope, we recommend that you study this manual thoroughly before operating this microscope, and always keep this manual at hand when operating this product.
  • Page 2 Refer to your local Olympus distributor in EU for return and/or collection systems available in your country. NOTE: This product has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

  • Page 3: Table Of Contents

    Contents Safety precautions ............................1 Standard combination......................... 2 Nomenclature of operating portions ..................3 Outline of brightfield/darkfield observation methods ..........4 Observation procedures ........................Turning ON the LED illumination ............................Selection between the eyepiece light path and the camera light path ........Placing the specimen ................................
  • Page 4 9 Assembly ............................... 9-1 Assembly diagram ............................9-2 Assembly procedures ..........................Removing the standard 10X eyepiece ........................Attaching the eyepiece micrometer .......................... Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H) ........... Attaching the objective CXPL20X or CXPL100XO ..................Attaching the specimen holder CX3-SHP or CX3-HLDT ................. Attaching the camera adapter U-TV1XC and the camera ..............

  • Page 5: Safety Precautions

    CX33 Safety precautions If the product is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the product may also be damaged. Always use the product according to this instruction manual.
  • Page 6 LED for a long time while feeling too bright, since it may cause damage to your eyes. CAUTION - Electric safety - Always use the AC adapter and power cord provided by Olympus. If the proper AC adapter and the power cord are not used, the electric safety and the EMC (Electro-Magnetic Compatibility) performance of the product cannot be assured.
  • Page 7 CX33 Handling precautions · This product is a precision instrument. Handle it with care NOTE and avoid subjecting it to a sudden or severe impact. · Never disassemble any part of the product. Otherwise, failure could be caused. · The objectives are screwed in tightly to prevent them from being loosened during transportation.
  • Page 8 Do not use a highly sealable cover, such as a plastic bag, etc. as a dust cover. The humidity in the NOTE microscope may increase to damage the product. When disposing of this product, be sure to follow the regulations and rules of your local government. Contact Olympus for any questions. Intended use This product has been designed to be used to observe magnified images of specimens in various routine work and research applications.

  • Page 9: Standard Combination

    CX33 Standard combination Refer to the drawing below and make sure that all necessary units are included in the product you purchased. Eyepieces Tube Objective Power cord Revolving nosepiece Stage Condenser AC adapter Microscope frame Option units · Eyepiece (2 pieces) ·...

  • Page 10: Nomenclature Of Operating Portions

    Nomenclature of operating portions Pre-focusing lever Coarse focusing knob Coarse focusing knob Fine focusing knob Fine focusing knob Binocular portion Binocular portion Main switch Main switch : Power is ON. : Power is OFF. Light path selection knob Light path selection knob Diopter adjustment rings Diopter adjustment rings Revolving nosepiece...

  • Page 11: Outline Of Brightfield/Darkfield Observation Methods

    CX33 Outline of brightfield/darkfield observation methods     (Operation portion)      (Page) Main switch 9  Set the main switch to (ON) and adjust the brightness. Brightness adjustment knob 9  Light path selection knob Select the light path. (Trinocular tube) Specimen holder Place the specimen on the stage.
  • Page 12 Make a copy of this observation procedure guide and put it near the microscope to use for observation.

  • Page 13: Observation Procedures

    CX33 Observation procedures Turning ON the LED illumination Set the main switch a to (ON). Rotating the brightness adjustment knob b in the arrow direction increases the brightness and rotating it in the opposite direction decreases the brightness. Selection between the eyepiece light path and the camera light path You can select the light path for observing the image with eyepieces or the light path for observing the image on monitors, etc.

  • Page 14: Placing The Specimen

    Placing the specimen Specimen holder for observing one specimen Rotate the coarse focusing knob a in the arrow direction to fully lower the stage. Press the specimen holding lever knob b backward (arrow direction) to open the lever c , and slide the specimen from front to back on the stage to place it.
  • Page 15 CX33 (Option) When using the specimen holder CX3-HLDT Rotate the coarse focusing knob a in the arrow direction to fully lower the stage. Press the specimen holding lever knob b backward (arrow direction) to open the lever c , and slide the specimen from front to back on the stage to place it.
  • Page 16 Moving the specimen · Rotating the upper Y-axis knob a moves the specimen in the Y-axis direction (front and back). Rotating the lower X-axis knob b moves the specimen in the X-axis · direction (right and left). Stage movable range: Depth 52 mm x Width 76 mm ·...
  • Page 17 CX33 Fixing the stage If you want to move the observation position by moving the specimen with your finger without using the specimen holder, the stage can be fixed so that it does not move unexpectedly. Move the X-axis/Y-axis knobs to match the hole a at the back right of the stage with the screw hole b .

  • Page 18: Selecting The Objective

    Selecting the objective Hold the revolving nosepiece a and rotate it so that the intended objective comes exactly above the specimen. · Do not rotate the revolving nosepiece by holding the NOTE objective. · Be careful if you rotate the revolving nosepiece while observing the edge of the slide glass with the high magnification objective (40X, etc.), the objective may interfere with the specimen holder.

  • Page 19: Adjusting The Interpupillary Distance

    CX33 Using the pre-focusing lever The pre-focusing lever prevents the specimen from being damaged by collision between the specimen and objective. After bringing the specimen into focus with the high magnification objective, rotate the pre-focusing lever a in the arrow direction until it stops.

  • Page 20: Adjusting The Diopter

    Adjusting the diopter. The diopter adjustment is to compensate for the difference in eyesights of left and right eyes of the observer. Rotate the diopter adjustment rings a of the right and left eyepieces and move each scale “0” to each index b . Engage the 10X objective in the light path and rotate the coarse/fine focusing knobs to bring the specimen into focus.

  • Page 21: Adjusting The Aperture Diaphragm (As)

    CX33 Using the eye shades When wearing eyeglasses Use the eye shades in the normal, folded-down position. When not wearing eyeglasses Extend the folded eye shades in the arrow direction. Since the eye shades prevent the unnecessary light from entering between eyepieces and eyes, you can observe the specimen comfortably.

  • Page 22: Acquiring The Image With The Camera

    Acquiring the image with the camera The observed image can be acquired by attaching the camera adapter and the digital camera for microscope to the trinocular tube. (For attaching the camera adapter and the camera, see page 30.) When using the camera adapter, be sure to adjust the parfocality. Otherwise, the image through NOTE eyepieces and the image acquired by the camera are not focused at the same position.

  • Page 23: Using The 100X Oil Immersion Objective

    CX33 Using the 100X oil immersion objective · Apply the immersion oil specified by Olympus to the tip of the 100X oil immersion objective. NOTE Otherwise, the observed image is not in focus. · Always use the immersion oil provided by Olympus.
  • Page 24 After use, lower the stage and rotate the revolving nosepiece, and remove the objective attached with the immersion oil from the specimen. Wipe off the immersion oil thoroughly from the tip of the objective and the tip of the condenser lens with the cleaning paper or the gauze slightly moistened with absolute alcohol.

  • Page 25: Glossary Of Optical Performance Terminology

    CX33 Glossary of optical performance terminology Total magnification The size of the specimen image to be observed is obtained by multiplying the eyepiece magnification by the objective magnification. This value is referred to as the total magnification. Example: Eyepiece (10X) x Objective (40X) = 400X Resolution The resolution is the ability of the lens to separate the image created by multiple proximal points.
  • Page 26 Aperture diaphragm The aperture diaphragm is a diaphragm to adjust the numerical aperture of the condenser. Adjusting the numerical aperture of the condenser appropriately with respect to the numerical aperture of each objective allows you to observe the specimen with the best contrast and resolution. In general, since the contrast of the specimen to be observed 70-80% with microscope is low, it is appropriate to adjust the...

  • Page 27: Troubleshooting

    CX33 Troubleshooting If problems occur, please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list, please contact Olympus for assistance. Problem Cause Remedy Page The brightness of observed field...
  • Page 28 Repair request If you cannot solve the problems even though taking actions described in “Troubleshooting”, please contact Olympus for assistance. Please provide us the following information at that time. Product name and abbreviation (Ex.: Biological Microscope CX33RTFS2)

  • Page 29: Specifications

    CX33 Specifications Item Specification Optical system Infinity optical system Microscope frame CX33RTFS2 / CX33LTFS2 Illumination system Built-in LED light source Microscope frame (rated input power): 5 V 0.85 A AC adapter (rated input power): 100-240 V 50-60 Hz 0.4 A AC adapter (rated output power): 5 V 2.5 A...

  • Page 30: List Of Optical Performances

    List of optical performances Magnification The following table shows the optical performances when combining eyepieces and Numerical aperture objectives. Mechanical tube The picture on the right shows the various length performances indicated on the objectives. Field number Color band Cover glass thickness Objective type (on the back side) Plan objective Optical...

  • Page 31: Assembly

    CX33 Assembly 9-1 Assembly diagram The number in the following diagram indicates the order to attach each unit. The detail assembly procedures are described on and after next page. Camera DP22 / DP27 (Option) Standard 10X eyepiece Camera adapter (detachable)

  • Page 32: 9-2 Assembly Procedures

    9-2 Assembly procedures Removing the standard 10X eyepiece The standard 10X eyepieces are clamped with screws. Loosen the clamping screws a of the 10X eyepieces using the small flathead screwdriver and remove the eyepieces. Attaching the eyepiece micrometer The size of the eyepiece micrometer which can be attached to eyepieces of this product is 24 mm in diameter and 1.5 mm in thickness.

  • Page 33: Attaching The Eyepieces (Standard 10X Eyepieces Or Whsz15X-H)

    CX33 Attaching the eyepieces (Standard 10X eyepieces or WHSZ15X-H) Insert WHC15X-H into the eyepiece sleeves and tighten the clamping screws a using the flathead screwdriver. Attaching the objective CXPL20X or CXPL100XO Screw in the objective to the mount hole of the revolving nosepiece completely by rotating it in the arrow direction paying attention not to drop it.

  • Page 34: Attaching The Specimen Holder Cx3-Shp Or Cx3-Hldt

    Attaching the specimen holder CX3-SHP or CX3-HLDT Rotate the coarse focusing knob to fully lower the stage. Loosen the specimen holder fixing knob a (2 pcs.) of the stage plate and remove the standard specimen holder by pulling it toward front side.

  • Page 35: Attaching The Filter Holder Ch2-Fh And Darkfield Ring Slit Ch2-Ds

    CX33 Attaching the filter holder CH2-FH and darkfield ring slit CH2-DS For details, see page 17. Attaching the filter of 45mm in diameter If necessary, insert the filter a in the filter holder (window lens) at the base of the microscope frame.

  • Page 36: Connecting The Ac Adapter And Power Cord

    Connecting the AC adapter and power cord · Always use the AC adapter and power cord provided by Olympus. If the proper AC adapter and the CAUTION power cord are not used, the electric safety and the EMC (Electro-Magnetic Compatibility) performance of the product cannot be assured.

  • Page 37: Attaching The Allen Wrench Holder

    CX33 Attaching the allen wrench holder · It is recommended to attach the provided allen wrench holder a to the back of the microscope frame. · The provided allen wrench b can be fixed to the provided allen wrench holder a .
  • Page 38 If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to “Specifications” and “Certified Cord” below: Caution : In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the electrical safety of the equipment.
  • Page 39 CX33 Table 2 HAR flexible cord Approval organizations and cordage harmonization marking methods Alternative marking utilizing Printed or embossed black-red-yellow thread (Length harmonization marking (May be of color section in mm) Approval organization located on jacket or insulation of internal wiring)
  • Page 40 Manufactured by Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan Distributed by 48 Woerd Avenue Waltham, MA 02453, U.S.A. 8F Olympus Tower, 446 Bongeunsa-ro, Gangnam-gu, Seoul, 06153 Korea Distributor Tengrant Inc. Tel.: +86(021)5428 6005 Fax: +86(021)5428 6005-8002 www.tengrant.com AX8881 02...

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